Biodegradable Alginate-Chitosan Hollow Nanospheres for Codelivery of Doxorubicin and Paclitaxel for the Effect of Human Lung Cancer A549 Cells

Abstract

A biodegradable alginate coated chitosan hollow nanosphere (ACHN) was prepared by a hard template method and used for codelivery of doxorubicin (DOX) and paclitaxel (PTX) to investigate the effect on human lung cancer A549 cells. PTX was loaded into the nanometer hollow structure of ACHN through adsorption method. DOX was coated on surface of ACHN through electrostatic interaction. Drug release studies exhibited a sustained-release effect. According to X-ray diffraction patterns (XRD), differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FT-IR) analysis, DOX structure in the loading samples (DOX-PTX-ACHN) was of amorphous state while PTX was microcrystalline. Cytotoxicity experiments showed ACHN was nontoxic as carrier material and the combination of DOX and PTX in DOX-PTX-ACHN exhibited a good inhibiting effect on cell proliferation. Cell uptake experiments demonstrated that DOX-PTX-ACHN accumulated in the cytoplasm. Degradation experiments illustrated that ACHN was a biodegradable material. In summary, these results clearly indicate that ACHN can be utilized as a potential biomaterial to transport multiple drugs to be used in combination therapy.

Targeted Antitumor Mechanism of C-PC/CMC-CD55sp Nanospheres in HeLa Cervical Cancer Cells

“…The EE and LE of the nanospheres were determined (Yang et al, 2017). The nanospheres were characterized using FTIR (Abbas et al, 2018;Dong et al, 2018), electron microscopy (Shi et al, 2016;Nantachit et al, 2017;Tao et al, 2018), and laser particle size analysis (Couvreur et al, 2002;Zhang et al, 2015;Shi et al, 2016;Ahmad et al, 2018;Panchu et al, 2018;von Halling Laier et al, 2018). The results showed that C-PC/CMC-CD55sp nanospheres were successfully constructed.…”

“…Encapsulation Efficiency (EE) and Loading Efficiency (LE) of C-PC in nanospheres were calculated as follows (Yang et al, 2017): EE = weight of C − PC in the nanospheres initial weight of C − PC  100 % LE = weight of C − PC in the nanospheres weight of the nanospheres  100 % Nanospheres were evaluated using FTIR spectra across the range of 500-4,000 cm -1 (Abbas et al, 2018;Dong et al, 2018). Nanospheres morphology was observed using a transmission electron microscope (Shi et al, 2016;Nantachit et al, 2017;Tao et al, 2018). Zeta potential (surface charge) and particle size (nanospheres size) were measured using a laser particle size analyzer at 25°C (Zhang et al, 2015;Ahmad et al, 2018;Panchu et al, 2018;von Halling Laier et al, 2018).…”

A review on chitosan and its development as pulmonary particulate anti-infective and anti-cancer drug carriers

“… Nil Co-delivery of paclitaxel and doxorubicin can effectively inhibit cell proliferation and promote cell apoptosis due to the nanoscale effect of particles and the synergistic effect of combined drugs. ( Tao et al, 2018 ) F10 A hybrid system composed of multi-walled carbon nanotubes (MWCNT) coated with chitosan is produced as a pH-responsive carrier of methotrexate. MWCNT are synthesized by fixed-bed chemical vapour deposition method.…”

“…The cytotoxic effects of chitosan are characterized by apoptosis via the activation of caspase 3 and caspase 8 ( Almutairi et al, 2019 ; Bharathi et al, 2019 ). The development of chitosan as pulmonary drug delivery carrier and as conjugate backbone of lung cancer therapy has been widely explored and gained successful outcomes ( Bharathi et al, 2019 ; Chi et al, 2019 ; Cirillo et al, 2019 ; Li et al, 2019 ; Pandey et al, 2019 ; Tao et al, 2018 ; Wang et al, 2017 ). The chitosan based pulmonary drug delivery carrier has been shown to reduce systemic toxicity of anti-cancer drugs, increase drug bioavailability in lungs and improve anti-tumor efficacy of the drugs.…”

Overview on natural hydrophilic polysaccharide polymers in drug delivery

“…Alginate‐coated chitosan hollow nanosphere showed any toxicity and exhibited a sustained‐release effect according to differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FT‐IR) analysis, and X‐ray diffraction patterns (XRD). Their results indicated that alginate‐coated chitosan hollow nanosphere can be applied for transport multiple drugs in combination therapy …”