Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida BET001 and modeling of its fermentation in shake flasks

Munawar, Khalil Munawar Makhdum; Simarani, Khanom; Annuar, Mohamad Suffian Mohamad

doi:10.1016/j.ejbt.2015.07.005

Cited by 15 publications

(9 citation statements)

References 15 publications

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“…The demonstration of which is available in Section 5 of Supporting information. These magnitudes of the

q_{PR}^{C \lim}

coefficients are consistent with those reported for other P. putida strains …”

Section: Resultssupporting

confidence: 90%

“…These magnitudes of the q C lim PR coefficients are consistent with those reported for other P. putida strains. 13,28 It is known that nitrogen-limiting growth conditions promote the accumulation of mcl-PHA, whereby a substrate fraction was required for product formation, resulting in a lower Y N lim P∕S 1 than that obtained under carbon limitation as D decreased [ Fig. 1(a)].…”

Section: Model Calibration For the Mcl-pha Production On Octanoatementioning

confidence: 99%

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A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

Vega

Castillo

2018

J of Chemical Tech & Biotech

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BACKGROUND Poly(hydroxyalkanoates) (PHAs) are biodegradable polymers that can replace conventional plastics, but microbial production of PHAs must be optimized for commercial success. The aim of this study is to provide a simple mathematical model based on previous studies in the literature to represent the production of PHAs in chemostat and batch cultures by Pseudomonas putida GPo1 (ATCC 29347) and Cupriavidus necator (DSM 545) grown on octanoate and glucose, respectively. RESULTS Kinetic and stoichiometric equations, dependent on the specific growth rate of residual biomass (µR), were developed for carbon‐ and nitrogen‐limiting growth conditions. The parameters have biochemical significance and are independent of the biomass concentration. The results revealed that the Luedeking–Piret model is growth‐associated for both fermentations under carbon limitation. In addition, the PHA content increases with µR. Meanwhile, the PHA content is amplified under nitrogen limitation but decreases as μR increases. Also, the Luedeking–Piret model exhibits a profile that depends on the synthesized polyhydroxyalkanoate. CONCLUSION This study demonstrates that strategies for the production of PHAs require an in‐depth understanding of the process kinetics. This model gives satisfactory predictions, may be extended to fed batch cultures and may be adapted to other fermentations. © 2018 Society of Chemical Industry

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“…The demonstration of which is available in Section 5 of Supporting information. These magnitudes of the

q_{PR}^{C \lim}

coefficients are consistent with those reported for other P. putida strains …”

Section: Resultssupporting

confidence: 90%

Section: Model Calibration For the Mcl-pha Production On Octanoatementioning

confidence: 99%

A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

Vega

Castillo

2018

J of Chemical Tech & Biotech

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“…136 Palm oil as a substrate for mcl-PHA production has been used in various studies. 130,137,138 For example, palm kernel oil was used as an inexpensive renewable substrate for mcl-PHA production using P. putida BET001 in a study performed by Munawar et al 137 In another investigation, crude sludge palm oil was employed to produce almost 41% of elastomeric mcl-PHA using P. putida S12. 138 Chen et al 130 used palm kernel oil and soybean oils for producing 50% CDW mcl-PHAs containing mainly 3HO, 3HD, and 3HDD.…”

Section: ■ Substrates For Mcl-pha Productionmentioning

confidence: 99%

A Review of Current Achievements and Recent Challenges in Bacterial Medium-Chain-Length Polyhydroxyalkanoates: Production and Potential Applications

Azizi,

Eslami,

Goudarzi

et al. 2024

Biomacromolecules

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Using petroleum-derived plastics has contributed significantly to environmental issues, such as greenhouse gas emissions and the accumulation of plastic waste in ecosystems.Researchers have focused on developing ecofriendly polymers as alternatives to traditional plastics to address these concerns. This review provides a comprehensive overview of medium-chain-length polyhydroxyalkanoates (mcl-PHAs), biodegradable biopolymers produced by microorganisms that show promise in replacing conventional plastics. The review discusses the classification, properties, and potential substrates of less studied mcl-PHAs, highlighting their greater ductility and flexibility compared to poly(3hydroxybutyrate), a well-known but brittle PHA. The authors summarize existing research to emphasize the potential applications of mcl-PHAs in biomedicine, packaging, biocomposites, water treatment, and energy. Future research should focus on improving production techniques, ensuring economic viability, and addressing challenges associated with industrial implementation. Investigating the biodegradability, stability, mechanical properties, durability, and cost-effectiveness of mcl-PHA-based products compared to petroleum-based counterparts is crucial. The future of mcl-PHAs looks promising, with continued research expected to optimize production techniques, enhance material properties, and expand applications. Interdisciplinary collaborations among microbiologists, engineers, chemists, and materials scientists will drive progress in this field. In conclusion, this review serves as a valuable resource to understand mcl-PHAs as sustainable alternatives to conventional plastics. However, further research is needed to optimize production methods, evaluate long-term ecological impacts, and assess the feasibility and viability in various industries.

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“…As substrate costs constitute the majority of the total production cost, substituting them with cheaper materials would be ideal. Therefore, fatty acid sources, including kernel oil, rapeseed oil, vegetable oil, waste palm oil, and waste cooking oil, could be potential substrates for economical carbon sources for mcl‐PHA production studies [ 18 , 39 , 47 , 48 , 52 ].…”

Section: Mcl‐pha Productionmentioning

confidence: 99%

Current trends in medium‐chain‐length polyhydroxyalkanoates: Microbial production, purification, and characterization

Hahn,

Alzate,

Leonhardt

et al. 2024

Engineering in Life Sciences

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Polyhydroxyalkanoates (PHAs) have gained interest recently due to their biodegradability and versatility. In particular, the chemical compositions of medium‐chain‐length (mcl)‐PHAs are highly diverse, comprising different monomers containing 6–14 carbon atoms. This review summarizes different feedstocks and fermentation strategies to enhance mcl‐PHA production and briefly discusses the downstream processing. This review also provides comprehensive details on analytical tools for determining the composition and properties of mcl‐PHA. Moreover, this study provides novel information by statistically analyzing the data collected from several reports on mcl‐PHA to determine the optimal fermentation parameters (specific growth rate, PHA productivity, and PHA yield from various structurally related and unrelated substrates), mcl‐PHA composition, molecular weight (MW), and thermal and mechanical properties, in addition to other relevant statistical values. The analysis revealed that the median PHA productivity observed in the fed‐batch feeding strategy was 0.4 g L−1 h−1, which is eight times higher than that obtained from batch feeding (0.05 g L−1 h−1). Furthermore, 3‐hydroxyoctanoate and ‐decanoate were the primary monomers incorporated into mcl‐PHA. The investigation also determined the median glass transition temperature (−43°C) and melting temperature (47°C), which indicated that mcl‐PHA is a flexible amorphous polymer at room temperature with a median MW of 104 kDa. However, information on the monomer composition or heterogeneity and the associated physical and mechanical data of mcl‐PHAs is inadequate. Based on their mechanical values, the mcl‐PHAs can be classified as semi‐crystalline polymers (median crystallinity 23%) with rubber‐like properties and a median elongation at break of 385%. However, due to the limited mechanical data available for mcl‐PHAs with known monomer composition, identifying suitable processing tools and applications to develop mcl‐PHAs further is challenging.

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Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida BET001 and modeling of its fermentation in shake flasks

Cited by 15 publications

References 15 publications

A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

A Review of Current Achievements and Recent Challenges in Bacterial Medium-Chain-Length Polyhydroxyalkanoates: Production and Potential Applications

Current trends in medium‐chain‐length polyhydroxyalkanoates: Microbial production, purification, and characterization

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