Bioconversion of anhydrosugars: Emerging concepts and strategies

Jarboe, Laura R.

doi:10.1002/iub.1533

Cited by 20 publications

(14 citation statements)

References 58 publications

(88 reference statements)

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“…Experimentally, anhydrosugars are highly bioavailable in oxic conditions, with a half-life of less than seven days. 25 The model may therefore not adequately account for enzymecatalyzed reactions, such as levoglucosan kinase or levoglucosan dehydrogenase, which cleave and phosphorylate the 1,6-intramolecular linkage, 74 and could be potentially common enzymes utilized by aquatic microorganisms. 75 Because of these nuances, the PyOM compound classes presented here are best used as bounding estimates for experimental validation, and for holistic comparison to NOM bioavailability.…”

Section: Resultsmentioning

confidence: 99%

Potential bioavailability of pyrogenic organic matter resembles natural dissolved organic matter pools

Graham¹,

Song²,

Samantha³

et al. 2021

Preprint

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Wildfires are increasing in severity and extent, creating many negative consequences for aquatic ecosystems. Pyrogenic materials generated by wildfires are transported across terrestrial landscapes into inland waters, where ~10% of organic matter pools may be comprised of black carbon (BC), a major component of pyrogenic organic matter (PyOM). Yet, the heterogeneity of PyOM from various fuels and burn conditions complicates efforts to understand its bioavailability. We used a substrate-explicit model to predict the energy content, metabolic efficiency, and rate of aerobic decomposition of representative PyOM compounds. This enabled us to systematically evaluate a full spectrum of PyOM chemistries that is unfeasible with laboratory experiments. The model relies on the elemental stoichiometry, allowing comparison of known PyOM chemistry to formula assignments of natural organic matter (NOM) from a recent high resolution mass spectrometry assessment of global aquatic NOM. Overall, we found the range of predicted bioavailability of PyOM was similar to NOM. Phenolic and BC molecules had lower metabolic efficiency than other PyOM and NOM compounds, and BC metabolism was less negatively impacted by oxygen limitation. In total, our work supports the recent paradigm shift regarding PyOM bioavailability, highlighting its potential role in global C emissions as the prevalence of wildfires increases.

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Section: Resultsmentioning

confidence: 99%

Potential bioavailability of pyrogenic organic matter resembles natural dissolved organic matter pools

Graham¹,

Song²,

Samantha³

et al. 2021

Preprint

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“…The aqueous streams from fast pyrolysis, in particular, are usually rich in levoglucosan, cellobiosan, HMF, furfural, and small acids (e.g., acetate) ( Black et al, 2016 , Johnston and Brown, 2014 , Patwardhan et al, 2009 , Pollard et al, 2012 , Remón et al, 2014 , Rover et al, 2013 , Valle et al, 2013 , Vispute and Huber, 2009 , Vispute et al, 2010 ). The microbial conversion of these types of substrates, dubbed “hybrid processing” by Brown, Jarboe, and colleagues, is far less studied than the conversion of biomass-derived sugars ( Bacik and Jarboe, 2016 , Brown, 2005 , Brown, 2007 , Jarboe et al, 2011b , Shen et al, 2015 ). The use of biological approaches to convert pyrolysis-derived compounds has almost solely focused to date on the conversion of levoglucosan and acetate, both highly prevalent intermediates from pyrolysis, into ethanol or natural carbon storage products such as fatty acids or polyhydroxyalkanoates in green algae, oleaginous yeast, model microbes such as Escherichia coli , or robust soil microbes such P. putida KT2440 ( Chi et al, 2013 , Dalluge et al, 2014 , Lian et al, 2010 , Lian et al, 2016 , Lian et al, 2013 , Lian et al, 2012 , Liang et al, 2013 , Linger et al, 2016 , Rover et al, 2014 , Zhao et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440

Guarnieri

Franden

Johnson

et al. 2017

Metabolic Engineering Communications

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The sugar dehydration products, furfural and 5-(hydroxymethyl)furfural (HMF), are commonly formed during high-temperature processing of lignocellulose, most often in thermochemical pretreatment, liquefaction, or pyrolysis. Typically, these two aldehydes are considered major inhibitors in microbial conversion processes. Many microbes can convert these compounds to their less toxic, dead-end alcohol counterparts, furfuryl alcohol and 5-(hydroxymethyl)furfuryl alcohol. Recently, the genes responsible for aerobic catabolism of furfural and HMF were discovered in Cupriavidus basilensis HMF14 to enable complete conversion of these compounds to the TCA cycle intermediate, 2-oxo-glutarate. In this work, we engineer the robust soil microbe, Pseudomonas putida KT2440, to utilize furfural and HMF as sole carbon and energy sources via complete genomic integration of the 12 kB hmf gene cluster previously reported from Burkholderia phytofirmans. The common intermediate, 2-furoic acid, is shown to be a bottleneck for both furfural and HMF metabolism. When cultured on biomass hydrolysate containing representative amounts of furfural and HMF from dilute-acid pretreatment, the engineered strain outperforms the wild type microbe in terms of reduced lag time and enhanced growth rates due to catabolism of furfural and HMF. Overall, this study demonstrates that an approach for biological conversion of furfural and HMF, relative to the typical production of dead-end alcohols, enables both enhanced carbon conversion and substantially improves tolerance to hydrolysate inhibitors. This approach should find general utility both in emerging aerobic processes for the production of fuels and chemicals from biomass-derived sugars and in the biological conversion of high-temperature biomass streams from liquefaction or pyrolysis where furfural and HMF are much more abundant than in biomass hydrolysates from pretreatment.

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“…The conversion of levoglucosan in eukaryotic microorganisms such as yeast and fungi has only thus far been shown to be carried out by the actions of a levoglucosan kinase [21,35]. Knowledge of the levoglucosan dehydrogenase metabolic pathway is limited, however it catalyses the conversion of levoglucosan in the presence of adenosine triphosphate (ATP) and Mg 2+ and the enzymatic reaction involves the simultaneous hydrolysis and phosphorylation of the substrate, yielding d-glucose-6-phosphate and adenosine diphosphate (ADP) [38,39]. Researchers have successfully been able to isolate and integrate endogenous levoglucosan kinase genes from yeast or fungi into hosts such as E. coli to then investigate levoglucosan consumption rates and product development [8,21,22].…”

Section: Discussionmentioning

confidence: 99%

Identification of Bio-oil Compound Utilizing Yeasts Through Phenotypic Microarray Screening

Kostas

Cooper

Shepherd

et al. 2019

Waste Biomass Valor

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Biomass pyrolysis bio-oil contains a plethora of carbon sources with the potential to be utilized by microorganisms and converted into high value products. However, the majority of these compounds are either highly toxic to microorganisms or are not directly utilizable. Hence research is required to develop methods of separating microbe friendly compounds from inhibitory ones, and to also identify novel microorganisms that can utilise the principal carbon sources in pyrolysis bio-oil. This study employed a phenotypic microarray (PM) technique that measured yeast metabolic output to screen for and shortlist yeast strains able to metabolize various bio-oil carbon sources, with a focus on the anhydrosugar levoglucosan. Four strains of yeast (two Pichia spp. and two Kluyveromyces spp.) were shortlisted due to their high metabolic output (between 79.7 and 113.7 redox signal intensity) on levoglucosan from the PM assay. Under anaerobic fermentation conditions the strains were able to uptake levoglucosan (between 79 and 100% uptake efficiency) but not produce bioethanol; yet trace amounts of acetic acid were generated. This study demonstrated the application of applying the PM technique to screen for novel yeast strains with abilities to metabolize compounds present in pyrolysis bio-oil that could lead to the identification of novel levoglucosan utilization pathways.

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Bioconversion of anhydrosugars: Emerging concepts and strategies

Cited by 20 publications

References 58 publications

Potential bioavailability of pyrogenic organic matter resembles natural dissolved organic matter pools

Potential bioavailability of pyrogenic organic matter resembles natural dissolved organic matter pools

Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440

Identification of Bio-oil Compound Utilizing Yeasts Through Phenotypic Microarray Screening

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