Biocontrol of wilt-nematode complex infecting gerbera by Bacillus subtilis under protected cultivation

Ramyabharathi, Sa.; Meena, K. Sankari; Rajendran, L.; Karthikeyan, G.; Jonathan, E. I.; Raguchander, T.

doi:10.1186/s41938-018-0027-2

Cited by 23 publications

(16 citation statements)

References 22 publications

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“…The lipopeptides might be most related to the surfactin family of the lipopeptides. Similar results were obtained by Ramyabharathi and co-authors on Bacillus subtilis Bbv57 producing surfactin and iturin, which were confirmed on TLC with Rf value of 0.3 for surfactin and 0.7 for iturin family while comparing with the standard (Sigma-Aldrich) [35]. Likewise, Yánez-Mendizábal and co-workers reported surfactin and iturin with Rf values 0.3 and 0.7 respectively [36].…”

Section: Product Characterizationsupporting

confidence: 82%

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Phulpoto

et al. 2020

Microb Cell Fact

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Background: Biosurfactants, being highly biodegradable, ecofriendly and multifunctional compounds have wide applications in various industrial sectors including environmental bioremediation. Surfactin, a member of lipopeptide family, which is considered as one of the most powerful biosurfactants due to its excellent emulsifying activities as well as environmental and therapeutic applications. Therefore, the aim of this study was to investigate the newly isolated bacterial strain S2MT for production of surfactin-like biosurfactants and their potential applications for oilcontaminated soil remediation. Results: In this study, the strain S2MT was isolated from lake sediment and was identified as Bacillus nealsonii based on transmitted electron microscopy (TEM) and 16S rRNA ribo-typing. The strain S2MT produced biosurfactant that reduced the surface tension (34.15 ± 0.6 mN/m) and displayed excellent emulsifying potential for kerosene (55 ± 0.3%). Additionally, the maximum biosurfactant product yield of 1300 mg/L was achieved when the composition of the culture medium was optimized through response surface methodology (RSM). Results showed that 2% glycerol and 0.1% NH 4 NO 3 were the best carbon/nitrogen substrates for biosurfactant production. The parameters such as temperature (30 °C), pH (8), agitation (100 rpm), NH 4 NO 3 (0.1%) and NaCl (0.5%) displayed most significant contribution towards surface tension reduction that resulted in enhanced biosurfactant yield. Moreover, the extracted biosurfactants were found to be highly stable at environmental factors such as salinity, pH and temperature variations. The biosurfactants were characterized as cyclic lipopeptides relating to surfactin-like isoforms (C 13-C 15) using thinlayer chromatography (TLC), Ultra high performance liquid chromatography and mass spectrometry (UHPLC-MS). The crude biosurfactant product displayed up to 43.6 ± 0.08% and 46.7 ± 0.01% remediation of heavy engine-oil contaminated soil at 10 and 40 mg/L concentrations, respectively. Conclusion: Present study expands the paradigm of surfactin-like biosurfactants produced by novel isolate Bacillus nealsonii S2MT for achieving efficient and environmentally acceptable soil remediation as compared to synthetic surfactants.

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Section: Product Characterizationsupporting

confidence: 82%

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Phulpoto

et al. 2020

Microb Cell Fact

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“…The presence of the lipopeptides, surfactin and iturin was confirmed (Ramyabharathi et al . ). Likewise, Adam et al .…”

Section: Biological Control Based On Bacillus Speciesmentioning

confidence: 97%

“…; Ramyabharathi et al . ). However, such studies are often speculative or fail to elaborate on how the secondary metabolites were detected or distinguished from primary metabolites.…”

Section: Using Metabolomics To Detect Possible Nematicidal Compoundsmentioning

confidence: 97%

“…Metabolites with antifungal activity could also hold potential as bionematicides as these biological activities are not always restricted to a single domain of life (bacteria, archaea and eukaryotes). This was demonstrated by Ramyabharathi et al (2018) who investigated the biocontrol potential of B. subtilis Bbv 57 (KF718836) against a wilt-RKN disease complex consisting of Fusarium oxysporum f. sp. gerberae (KM523669) and Meloidogyne incognita.…”

Section: Biological Control Based On Bacillus Speciesmentioning

confidence: 99%

“…Various studies have found Bacillus secondary metabolites to be responsible for the observed antagonism towards plant pathogens such as Meloidogyne (Mendoza et al 2008;Xiong et al 2015;Ramyabharathi et al 2018). However, such studies are often speculative or fail to elaborate on how the secondary metabolites were detected or distinguished from primary metabolites.…”

Section: Using Metabolomics To Detect Possible Nematicidal Compoundsmentioning

confidence: 99%

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Microbial metabolomics: essential definitions and the importance of cultivation conditions for utilizing Bacillus species as bionematicides

Horak

Engelbrecht

Rensburg

et al. 2019

J of Applied Microbiology

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Root‐knot nematodes are destructive phytopathogens that damage agricultural crops globally, and there is growing interest in the use of biocontrol based on rhizobacteria such as Bacillus to combat Meloidogyne species. It is hypothesized that nematicidal activity of Bacillus can be attributed to the production of secondary metabolites and hydrolytic enzymes. Yet, few studies have characterized these metabolites and their identities remain unknown. Others are speculative or fail to elaborate on how secondary metabolites were detected or distinguished from primary metabolites. Metabolites can be classified based on their origin as either intracellular or extracellular and based on their function, as either primary or secondary. Although this classification is in general use, the boundaries are not always well defined. An understanding of the secondary metabolite and hydrolytic enzyme classification of Bacillus species will facilitate investigations aimed at bionematicide development. This review summarizes the significance of Bacillus hydrolytic enzymes and secondary metabolites in bionematicide research and provides an overview of known classifications. The importance of appropriate cultivation conditions for optimum metabolite and enzyme production is also discussed. Finally, the use of metabolomics for the detection and identification of nematicidal compounds is considered.

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Microbial Biosurfactants for Green Agricultural Technology

Patel

Mukherjee

et al. 2023

Sustainable Agriculture Reviews

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Biocontrol of wilt-nematode complex infecting gerbera by Bacillus subtilis under protected cultivation

Cited by 23 publications

References 22 publications

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Microbial metabolomics: essential definitions and the importance of cultivation conditions for utilizing Bacillus species as bionematicides

Microbial Biosurfactants for Green Agricultural Technology

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