Biocontrol of Potato Common Scab is Associated with High <i>Pseudomonas fluorescens</i> LBUM223 Populations and Phenazine-1-Carboxylic Acid Biosynthetic Transcript Accumulation in the Potato Geocaulosphere

Arseneault, Tanya; Filion, Martin

doi:10.1094/phyto-01-16-0019-r

Cited by 40 publications

(23 citation statements)

References 46 publications

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“…Biofilm formation was assessed in the three P. brassicacearum strains according to the methods described by Arseneault et al (49). Briefly, P. brassicacearum strains (wild-type LBUM300, LBUM300ΔhcnC, and LBUM300ΔphlD) were grown at 25°C for 48 h in TSB.…”

Section: Methodsmentioning

confidence: 99%

Interaction between 2,4-Diacetylphloroglucinol- and Hydrogen Cyanide-Producing Pseudomonas brassicacearum LBUM300 and Clavibacter michiganensis subsp. michiganensis in the Tomato Rhizosphere

Paulin

Novinscak

Lanteigne

et al. 2017

Appl Environ Microbiol

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We have previously demonstrated that inoculation of tomato plants with 2,4-diacetylphloroglucinol (DAPG)-and hydrogen cyanide (HCN)-producing Pseudomonas brassicacearum LBUM300 could significantly reduce bacterial canker symptoms caused by Clavibacter michiganensis subsp. michiganensis. In this study, in order to better characterize the population dynamics of LBUM300 in the rhizosphere of tomato plants, we characterized the role played by DAPG and HCN production by LBUM300 on rhizosphere colonization of healthy and C. michiganensis subsp. michiganensis-infected tomato plants. The impact of C. michiganensis subsp. michiganensis presence on the expression of DAPG and HCN biosynthetic genes in the rhizosphere was also examined. In planta assays were performed using combinations of C. michiganensis subsp. michiganensis and wild-type LBUM300 or DAPG (LBUM300ΔphlD) or HCN (LBUM300ΔhcnC) isogenic mutant strains. Populations of LBUM300 and phlD and hcnC gene expression levels were quantified in rhizosphere soil at several time points up to 264 h postinoculation using culture-independent quantitative PCR (qPCR) and reverse transcriptase quantitative PCR (RT-qPCR) TaqMan assays, respectively. The presence of C. michiganensis subsp. michiganensis significantly increased rhizospheric populations of LBUM300. In C. michiganensis subsp. michiganensis-infected tomato rhizospheres, the populations of wild-type LBUM300 and strain LBUM300ΔhcnC, both producing DAPG, were significantly higher than the population of strain LBUM300ΔphlD. A significant upregulation of phlD expression was observed in the presence of C. michiganensis subsp. michiganensis, while hcnC expression was only slightly increased in the mutant strain LBUM300ΔphlD when C. michiganensis subsp. michiganensis was present. Additionally, biofilm production was found to be significantly reduced in strain LBUM300ΔphlD compared to the wild-type and LBUM300ΔhcnC strains. IMPORTANCEThe results of this study suggest that C. michiganensis subsp. michiganensis infection of tomato plants contributes to increasing rhizospheric populations of LBUM300, a biocontrol agent, as well as the overexpression of the DAPG biosynthetic operon in this bacterium. The increasing rhizospheric populations of LBUM300 represent one of the key factors in controlling C. michiganensis subsp. michiganensis in tomato plants, as DAPG-producing bacteria have shown the ability to decrease bacterial canker symptoms in tomato plants. KEYWORDS Clavibacter michiganensis, DAPG, HCN, Pseudomonas, gene expression, rhizosphere, tomato

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Section: Methodsmentioning

confidence: 99%

Interaction between 2,4-Diacetylphloroglucinol- and Hydrogen Cyanide-Producing Pseudomonas brassicacearum LBUM300 and Clavibacter michiganensis subsp. michiganensis in the Tomato Rhizosphere

Paulin

Novinscak

Lanteigne

et al. 2017

Appl Environ Microbiol

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“…Biofilm formation of the bacteria was assessed in vitro after 48 h of growth following the methods described by Arseneault et al (2016). Briefly, the optical density of bacterial cells was determined after staining cells with crystal violet and subsequent destaining with 95% ethanol following growth in M9 minimal salts medium.…”

Section: Biofilm Assaymentioning

confidence: 99%

Enhancing total lipid and stearidonic acid yields inBuglossoides arvensisthrough PGPR inoculation

Novinscak

Filion

2018

J Appl Microbiol

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“…(Mavrodi et al, 2012a ). Phenazines produced by 2-79 and closely related species of Phz + pseudomonads exhibit broad-spectrum antibiotic activity and contribute to the capacity of these organisms to suppress several important plant pathogens (Thomashow and Weller, 1988 ; Chin-a-Woeng et al, 1998 ; Arseneault et al, 2016 ; Jaaffar et al, 2017 ). Our surveys of commercial wheat fields from throughout the Columbia Plateau revealed that the frequency of root systems colonized by Phz + bacteria is inversely correlated with annual precipitation and irrigation (Mavrodi et al, 2012a , b ).…”

Section: Introductionmentioning

confidence: 99%

Long-Term Irrigation Affects the Dynamics and Activity of the Wheat Rhizosphere Microbiome

Mavrodi

Elbourne

et al. 2018

Front. Plant Sci.

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The Inland Pacific Northwest (IPNW) encompasses 1. 6 million cropland hectares and is a major wheat-producing area in the western United States. The climate throughout the region is semi-arid, making the availability of water a significant challenge for IPNW agriculture. Much attention has been given to uncovering the effects of water stress on the physiology of wheat and the dynamics of its soilborne diseases. In contrast, the impact of soil moisture on the establishment and activity of microbial communities in the rhizosphere of dryland wheat remains poorly understood. We addressed this gap by conducting a three-year field study involving wheat grown in adjacent irrigated and dryland (rainfed) plots established in Lind, Washington State. We used deep amplicon sequencing of the V4 region of the 16S rRNA to characterize the responses of the wheat rhizosphere microbiome to overhead irrigation. We also characterized the population dynamics and activity of indigenous Phz+ rhizobacteria that produce the antibiotic phenazine-1-carboxylic acid (PCA) and contribute to the natural suppression of soilborne pathogens of wheat. Results of the study revealed that irrigation affected the Phz+ rhizobacteria adversely, which was evident from the significantly reduced plant colonization frequency, population size and levels of PCA in the field. The observed differences between irrigated and dryland plots were reproducible and amplified over the course of the study, thus identifying soil moisture as a critical abiotic factor that influences the dynamics, and activity of indigenous Phz+ communities. The three seasons of irrigation had a slight effect on the overall diversity within the rhizosphere microbiome but led to significant differences in the relative abundances of specific OTUs. In particular, irrigation differentially affected multiple groups of Bacteroidetes and Proteobacteria, including taxa with known plant growth-promoting activity. Analysis of environmental variables revealed that the separation between irrigated and dryland treatments was due to changes in the water potential (Ψm) and pH. In contrast, the temporal changes in the composition of the rhizosphere microbiome correlated with temperature and precipitation. In summary, our long-term study provides insights into how the availability of water in a semi-arid agroecosystem shapes the belowground wheat microbiome.

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Biocontrol of Potato Common Scab is Associated with High Pseudomonas fluorescens LBUM223 Populations and Phenazine-1-Carboxylic Acid Biosynthetic Transcript Accumulation in the Potato Geocaulosphere

Cited by 40 publications

References 46 publications

Interaction between 2,4-Diacetylphloroglucinol- and Hydrogen Cyanide-Producing Pseudomonas brassicacearum LBUM300 and Clavibacter michiganensis subsp. michiganensis in the Tomato Rhizosphere

Interaction between 2,4-Diacetylphloroglucinol- and Hydrogen Cyanide-Producing Pseudomonas brassicacearum LBUM300 and Clavibacter michiganensis subsp. michiganensis in the Tomato Rhizosphere

Enhancing total lipid and stearidonic acid yields inBuglossoides arvensisthrough PGPR inoculation

Long-Term Irrigation Affects the Dynamics and Activity of the Wheat Rhizosphere Microbiome

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