Interaction between 2,4-Diacetylphloroglucinol- and Hydrogen Cyanide-Producing Pseudomonas brassicacearum LBUM300 and Clavibacter michiganensis subsp. michiganensis in the Tomato Rhizosphere

Paulin, Mélanie M.; Novinscak, Amy; Lanteigne, Carine; Gadkar, Vijay; Filion, Martin

doi:10.1128/aem.00073-17

Cited by 30 publications

(15 citation statements)

References 50 publications

(60 reference statements)

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“…This is consistent with the results obtained on Solanum lycopersicum with 23 distinct Pseudomonas strains by Ghirardi et al (29) and on Pisum sativum with 14 strains by Landa et al (55). For S. tuberosum, the colonization levels observed were in the same range as in other studies using quantitative PCR to measure rhizosphere colonization by Pseudomonas strains (56,57). However, colonization was much lower in the rhizosphere of A. thaliana.…”

Section: Discussionsupporting

confidence: 91%

Metabolic and Genomic Traits of Phytobeneficial Phenazine-ProducingPseudomonasspp. Are Linked to Rhizosphere Colonization inArabidopsis thalianaandSolanum tuberosum

Zboralski

Biessy

Savoie

et al. 2020

Appl Environ Microbiol

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Bacterial rhizosphere colonization is critical for phytobeneficial rhizobacteria such as phenazine-producing Pseudomonas spp. To better understand this colonization process, potential metabolic and genomic determinants required for rhizosphere colonization were identified using a collection of 60 phenazine-producing Pseudomonas strains isolated from multiple plant species and representative of the worldwide diversity. Arabidopsis thaliana and Solanum tuberosum (potato) were used as host plants. Bacterial rhizosphere colonization was measured by quantitative PCR using a newly designed primer pair and TaqMan probe targeting a conserved region of the phenazine biosynthetic operon. The metabolic abilities of the strains were assessed on 758 substrates using Biolog phenotype microarray technology. These data, along with available genomic sequences for all strains, were analyzed in light of rhizosphere colonization. Strains belonging to the P. chlororaphis subgroup colonized the rhizospheres of both plants more efficiently than strains belonging to the P. fluorescens subgroup. Metabolic results indicated that the ability to use amines and amino acids was associated with an increase in rhizosphere colonization capability in A. thaliana and/or in S. tuberosum. The presence of multiple genetic determinants in the genomes of the different strains involved in catabolic pathways and plant-microbe and microbe-microbe interactions correlated with increased or decreased rhizosphere colonization capabilities in both plants. These results suggest that the metabolic and genomic traits found in different phenazine-producing Pseudomonas strains reflect their rhizosphere competence in A. thaliana and S. tuberosum. Interestingly, most of these traits are associated with similar rhizosphere colonizing capabilities in both plant species. IMPORTANCE Rhizosphere colonization is crucial for plant growth promotion and biocontrol by antibiotic-producing Pseudomonas spp. This colonization process relies on different bacterial determinants which partly remain to be uncovered. In this study, we combined a metabolic and a genomic approach to decipher new rhizosphere colonization determinants which could improve our understanding of this process in Pseudomonas spp. Using 60 distinct strains of phenazine-producing Pseudomonas spp., we show that rhizosphere colonization abilities correlated with both metabolic and genomic traits when these bacteria were inoculated on two distant plants, Arabidopsis thaliana and Solanum tuberosum. Key metabolic and genomic determinants presumably required for efficient colonization of both plant species were identified. Upon further validation, these targets could lead to the development of simple screening tests to rapidly identify efficient rhizosphere colonizers.

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Section: Discussionsupporting

confidence: 91%

Metabolic and Genomic Traits of Phytobeneficial Phenazine-ProducingPseudomonasspp. Are Linked to Rhizosphere Colonization inArabidopsis thalianaandSolanum tuberosum

Zboralski

Biessy

Savoie

et al. 2020

Appl Environ Microbiol

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“…Accordingly, the reduction of canker disease development and Cmm population by Pseudomonas sp. LBUM300 is attributed to the production of the antibiotics 2,4‐diacetylphloroglucinol (DAPG) and HCN (Lanteigne et al ., ; Paulin et al ., ).…”

Section: Control Of Cmmmentioning

confidence: 97%

Clavibacter michiganensis ssp. michiganensis: bacterial canker of tomato, molecular interactions and disease management

Nandi

MacDonald

Liu

et al. 2018

Molecular Plant Pathology

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Bacterial canker disease is considered to be one of the most destructive diseases of tomato (Solanum lycopersicum), and is caused by the seed-borne Gram-positive bacterium Clavibacter michiganensis ssp. michiganensis (Cmm). This vascular pathogen generally invades and proliferates in the xylem through natural openings or wounds, causing wilt and canker symptoms. The incidence of symptomless latent infections and the invasion of tomato seeds by Cmm are widespread. Pathogenicity is mediated by virulence factors and transcriptional regulators encoded by the chromosome and two natural plasmids. The virulence factors include serine proteases, cell wall-degrading enzymes (cellulases, xylanases, pectinases) and others. Mutational analyses of these genes and gene expression profiling (via quantitative reverse transcription-polymerase chain reaction, transcriptomics and proteomics) have begun to shed light on their roles in colonization and virulence, whereas the expression of tomato genes in response to Cmm infection suggests plant factors involved in the defence response. These findings may aid in the generation of target-specific bactericides or new resistant varieties of tomato. Meanwhile, various chemical and biological controls have been researched to control Cmm. This review presents a detailed investigation regarding the pathogen Cmm, bacterial canker infection, molecular interactions between Cmm and tomato, and current perspectives on improved disease management.

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“…For example, 2,4-diacetylphloroglucinol-producing Pseudomonas species inhibit the growth of Clavibacter michiganensis subsp. michiganensis [ 60 ]. It was, thus, worthwhile to look at the Pseudomonas community in diseased and healthy leaves for potential explanation(s).…”

Section: Discussionmentioning

confidence: 99%

MinION Nanopore-based detection of Clavibacter nebraskensis, the corn Goss’s wilt pathogen, and bacteriomic profiling of necrotic lesions of naturally-infected leaf samples

Adam

Chapados

et al. 2021

PLoS ONE

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The Goss’s bacterial wilt pathogen, Clavibacter nebraskensis, of corn is a candidate A1 quarantine organism; and its recent re-emergence and spread in the USA and Canada is a potential biothreat to the crop. We developed and tested an amplicon-based Nanopore detection system for C. nebraskensis (Cn), targeting a purine permease gene. The sensitivity (1 pg) of this system in mock bacterial communities (MBCs) spiked with serially diluted DNA of C. nebraskensis NCPPB 2581T is comparable to that of real-time PCR. Average Nanopore reads increased exponentially from 125 (1pg) to about 6000 reads (1000 pg) after a 3-hr run-time, with 99.0% of the reads accurately assigned to C. nebraskensis. Three run-times were used to process control MBCs, Cn-spiked MBCs, diseased and healthy leaf samples. The mean Nanopore reads doubled as the run-time is increased from 3 to 6 hrs while from 6 to 12 hrs, a 20% increment was recorded in all treatments. Cn-spiked MBCs and diseased corn leaf samples averaged read counts of 5,100, 11,000 and 14,000 for the respective run-times, with 99.8% of the reads taxonomically identified as C. nebraskensis. The control MBCs and healthy leaf samples had 47 and 14 Nanopore reads, respectively. 16S rRNA bacteriomic profiles showed that Sphingomonas (22.7%) and Clavibacter (21.2%) were dominant in diseased samples while Pseudomonas had only 3.5% relative abundance. In non-symptomatic leaf samples, however, Pseudomonas (20.0%) was dominant with Clavibacter at 0.08% relative abundance. This discrepancy in Pseudomonas abundance in the samples was corroborated by qPCR using EvaGreen chemistry. Our work outlines a new useful tool for diagnosis of the Goss’s bacterial wilt disease; and provides the first insight on Pseudomonas community dynamics in necrotic leaf lesions.

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Interaction between 2,4-Diacetylphloroglucinol- and Hydrogen Cyanide-Producing Pseudomonas brassicacearum LBUM300 and Clavibacter michiganensis subsp. michiganensis in the Tomato Rhizosphere

Cited by 30 publications

References 50 publications

Metabolic and Genomic Traits of Phytobeneficial Phenazine-ProducingPseudomonasspp. Are Linked to Rhizosphere Colonization inArabidopsis thalianaandSolanum tuberosum

Metabolic and Genomic Traits of Phytobeneficial Phenazine-ProducingPseudomonasspp. Are Linked to Rhizosphere Colonization inArabidopsis thalianaandSolanum tuberosum

Clavibacter michiganensis ssp. michiganensis: bacterial canker of tomato, molecular interactions and disease management

MinION Nanopore-based detection of Clavibacter nebraskensis, the corn Goss’s wilt pathogen, and bacteriomic profiling of necrotic lesions of naturally-infected leaf samples

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