Biocontrol of Fusarium spp., Causal Agents of Damping-off in Cotton Plants by Native Bacillus subtilis Isolated from Prosopis juliflora

Abstract

The present study was conducted to evaluate the application of native phosphate solubilizing Bacillus sp., isolated from Prosopis juliflora rhizosphere in the biocontrol of three different Fusarium species. The native Bacillus sp., was further confirmed as B. subtilis using the Polymerase Chain Reaction (PCR). The results showed that the phosphate solubilizing ability of B. subtilis strain ALICA was tested in liquid medium with 0.5% Ca3(PO4)2, and maximum of the value of soluble phosphate was 202.02 µg/mL after 3 days of incubation. On the other hand, the growth inhibition of Fusarium solani, F. equiseti and F. oxysporum were more evident in cell-free medium filtrates with respect to dual medium with a range of percentage inhibition from 51, 66 and 47% after 5 days, respectively. Our result of the DNA-PCR amplification of the lipopeptide genes from B. subtilis strain ALICA, showed 94% identity with subtilosin and subtilisin of different Bacillus spp. This result turns B. subtilis strain ALICA into a potential alternative for the biological control of Fusarium species present in the soil of transgenic insect-resistant cotton plants in Mexico.

Assessment of the safety and anti‐inflammatory effects of three Bacillus strains in the respiratory tract

“…The presence of the sbo gene encoding for subtilosin A in B. subtilis 3 and 281, a cyclic antimicrobial peptide previously reported for its activity against Gram‐positive and Gram‐negative bacteria (Shelburne et al ., 2006) suggests that most probably not only a single antimicrobial metabolite accounts for the observed antifungal and antibacterial activities in this study. Subtilosin A has been reported for different B. subtilis strains intended for use in the control of bacteria and fungi (Algburi et al ., 2016; Abdelmoteleb, 2017). Bacteriocins have been well described for different Bacillus species, including B. subtilis and B. amyloliquefaciens (Shelburne et al ., 2006; Masuda et al ., 2011; Field et al ., 2015).…”

Antifungical Activity of AutochthonousBacillus subtilisIsolated fromProsopis julifloraagainst Phytopathogenic Fungi

“…B. subtilis strains “ALICA” (GeneBank No. KX137176) used in the present study was previously isolated from rhizosphere soil of P. juliflora [ 9 ]. The plant pathogenic fungi A. alternata , Macrophomina sp., C. gloeosporioides , Botrytis cinerea , and S. rolfesii were kindly supplied by Dra.…”

Biocontrol of Fusarium equiseti using chitosan nanoparticles combined with Trichoderma longibrachiatum and Penicillium polonicum

“…For instance, Azotobacter nigricans was reported as antifungal against some Fusarium species including F. sporotrichioides, F. graminearum, F. poae and F. equiseti with a growth inhibition up to 50% [ 19 ]. Bacillus subtilis was used for controlling F. solani, F. equiseti and F. oxysporum with inhibition percentage ranged from 51, 66 and 47% after 5 days, respectively [ 20 ]. Also, Streptomyces bellus was applied against F. equiseti and two strains of F. fujikuroi and achieved inhibition percentage 55, 43 and 36%, respectively [ 16 ].…”