Antifungical Activity of AutochthonousBacillus subtilisIsolated fromProsopis julifloraagainst Phytopathogenic Fungi

Abstract

The ability of Bacillus subtilis, strain ALICA to produce three mycolytic enzymes (chitinase, β-1,3-glucanase, and protease), was carried out by the chemical standard methods. Bacillus subtilis ALICA was screened based on their antifungal activity in dual plate assay and cell-free culture filtrate (25%) against five different phytopathogenic fungi Alternaria alternata, Macrophomina sp., Colletotrichum gloeosporioides, Botrytis cinerea, and Sclerotium rolfesii. The B. subtilis ALICA detected positive for chitinase, β-1,3-glucanase and protease enzymes. Fungal growth inhibition by both strain ALICA and its cell-free culture filtrate ranged from 51.36% to 86.3% and 38.43% to 68.6%, respectively. Moreover, hyphal morphological changes like damage, broken, swelling, distortions abnormal morphology were observed. Genes expression of protease, β-1,3-glucanase, and lipopeptides (subtilosin and subtilisin) were confirmed their presence in the supernatant of strain ALICA. Our findings indicated that strain ALICA provided a broad spectrum of antifungal activities against various phytopathogenic fungi and may be a potential effective alternative to chemical fungicides.

Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata)

“…They have been isolated from a variety of plants, including legume plants as Medicago polymorPha (Chinnaswamy et al, 2018), Dalbergia odorifera (Lu et al, 2017), Glycine max (Hong et al, 2007), Cajanus cajan (Rajendran et al, 2007) , Hedysarum spinosissimum (Sbabou et al, 2016) and Prosopis species. Bacteria belonging to genus Bacillus have been isolated from the endosphere and rhizosphere of Prosopis juliflora (Khan et al, 2014; Abdelmoteleb et al, 2017) and Prosopis farcta nodules (Fterich et al, 2011). Though our Bacillus strains failed to induce nodule formation, the co-existence of Bacillus genus with rhizobial bacteria into legume nodule and the ability of Bacillus to influence the growth and fitness of plants has been well established (Arias et al, 2011).…”

Repurposing Anti-diabetic Drugs to Cripple Quorum Sensing in Pseudomonas aeruginosa

“…To evaluate the protease inhibitory activities of anti-diabetic drugs, the skim milk agar method was used [ 6 , 29 ]. P. aeruginosa treated with anti-diabetic drugs (sub-MIC) or untreated cultures were adjusted to an OD600 of 0.4, centrifuged at 21,000× g for 20 min.…”

Potential of root nodule nonrhizobial endophytic bacteria for growth promotion of Lotus corniculatus L. and Dactylis glomerata L.

“…The chitinolytic activity of isolates was tested on the media enriched with chitin (5 g l −1 ). Protease activity was tested on skim milk agar (Abdelmoteleb et al 2017). Isolates were spot inoculated on 10% skim milk agar and incubated for 7 days at 28°C, and a clear halo zone around bacterial growth was considered a proteolytic activity.…”