Biocontrol of Botrytis allii Munn the Causal Agent of Neck Rot, the Post Harvest Disease in Onion, by Use of a New Iranian Isolate of Streptomyces

Jorjandi,

doi:10.3844/ajab.2009.72.78

Cited by 9 publications

(9 citation statements)

References 4 publications

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“…Botrytis is known to be responsible for the gray rot of many foods while Mucor is involved in the post-harvest rots of many fruits and other foods. Moreover, species of the genera Penicillium, Aspergillus, Botrytis and Geotrichum are reported as common fungi to the post-harvest problems (Jorjandi et al, 2009;Naureen et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Assessment of the antifungal activities of polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant against fungi isolated from papaya (Carica papaya L.) fruit

Koffi-Névry¹

2011

Afr. J. Microbiol. Res.

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The antifungal activity of the Polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant against fungi isolated from papaya fruit was evaluated. The aim of this work was to show that the PHMGH can be used as a disinfectant for papaya preservation. Thirty one strains of fungi were isolated as Mucor sp., Botrytis sp., Penicillium sp., Geotrichum sp., Aspergillus sp. and Colletotrichum sp. Mucor sp. was the most isolated with a frequency of 52.77% followed by Botrytis (47.22%), Aspergillus and Penicillium (8.33%), Colletotrichum (5.56%) then, Geotrichum (2.78%). The antifungal activity of the PHMGH was tested through the determination of the minimal inhibitory concentrations (MICs) and the minimal fungicidal concentrations (MFCs). All the strains tested were sensitive to the disinfectant. However, the activity of PHMGH varies according to the strain tested. The values of the MIC and the MFC were between <0.01 and 1.9 mg/ mL. Aspergillus sp. was the least susceptible fungi with a MIC and MFC of 1.9 mg/ mL whereas the most sensitive to PHMGH were the genera Botrytis and Colletotrichum with a MIC <0.01 mg/ mL. No matter the strains of fungi studied, the MIC and MFC values were equal. The PHMGH could therefore be considered as a fungicidal agent that could serve for the preservation of papaya fruit after harvesting.

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Section: Discussionmentioning

confidence: 99%

Assessment of the antifungal activities of polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant against fungi isolated from papaya (Carica papaya L.) fruit

Koffi-Névry¹

2011

Afr. J. Microbiol. Res.

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“…Actinomycetes are one of the most efficient groups of natural bioactive metabolites, and they have been used as antibiotics, antitumor agents, antioxidants, anti-inflammatory agents, anti-infection agents, enzyme inhibitors, pesticides, plant-growth-promoting substances, and so on (Qin et al, 2011 ; Wang X. J. et al, 2013 ; Ashokvardhan et al, 2014 ; Kumar V. et al, 2014 ; Shivlata and Satyanarayana, 2015 ; Tan et al, 2016 ). It has been reported that actinomycetes have been used to protect plants against a wide range of phytopathogenic fungi, and produce cell-wall degrading enzymes, antifungal antibiotics, and plant growth promoters (Yuan and Crawford, 1995 ; El-Tarabily et al, 2000 ; Doumbou et al, 2002 ; Bressan, 2003 ; Cao et al, 2005 ; El-Tarabily and Sivasithamparam, 2006 ; Prapagdee et al, 2008 ; Jorjandi et al, 2009 ; Eccleston et al, 2010 ; Mingma et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil

Chen

Zhou

et al. 2018

Front. Microbiol.

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An actinomycete strain, CB-75, was isolated from the soil of a diseased banana plantation in Hainan, China. Based on phenotypic and molecular characteristics, and 99.93% sequence similarity with Streptomyces spectabilis NBRC 13424 (AB184393), the strain was identified as Streptomyces sp. This strain exhibited broad-spectrum antifungal activity against 11 plant pathogenic fungi. Type I polyketide synthase (PKS-I) and non-ribosomal peptide synthetase (NRPS) were detected, which were indicative of the antifungal compounds that Streptomyces sp. CB-75 could produce. An ethyl acetate extract from the strain exhibited the lowest minimum inhibitory concentration (MIC) against Colletotrichum musae (ATCC 96167) (0.78 μg/ml) and yielded the highest antifungal activity against Colletotrichum gloeosporioides (ATCC 16330) (50.0 μg/ml). Also, spore germination was significantly inhibited by the crude extract. After treatment with the crude extract of Streptomyces sp. CB-75 at the concentration 2 × MIC, the pathogenic fungi showed deformation, shrinkage, collapse, and tortuosity when observed by scanning electron microscopy (SEM). By gas chromatography-mass spectrometry (GC-MS) of the crude extract, 18 chemical constituents were identified; (Z)-13-docosenamide was the major constituent. Pot experiments showed that the incidence of banana seedlings was reduced after using Streptomyces sp. CB-75 treatment. The disease index was 10.23, and the prevention and control effect was 83.12%. Furthermore, Streptomyces sp. CB-75 had a growth-promoting effect on banana plants. The chlorophyll content showed 88.24% improvement, the leaf area, root length, root diameter, plant height, and stem showed 88.24, 90.49, 136.17, 61.78, and 50.98% improvement, respectively, and the shoot fresh weight, root fresh weight, shoot dry weight, and root dry weight showed 82.38, 72.01, 195.33, and 113.33% improvement, respectively, compared with treatment of fermentation broth without Streptomyces sp. CB-75. Thus, Streptomyces sp. CB-75 is an important microbial resource as a biological control against plant pathogenic fungi and for promoting banana growth.

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“…To evaluate determination of minimum inhibitory concentration, twofold serial concentrations of 10, 5, 2.5, 1.25, 0.625, 0.312 and 0.156 mg/l of the crude extract were prepared in dimethyl sulfoxide (DM): methanol (1/1: v/v), and antibacterial activity was evaluated by well diffusion method (Jorjandi et al 2009). Three replicates were considered for each concentration.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

“…Regarding the preparation of crude extracts, 3 days after inoculation when the maximum activity was reached, spore and additional materials were removed by filtration through three layers of cheesecloth. The culture filtrates were freeze-dried and kept refrigerated for the next experiments (Jorjandi et al 2009;Sadeghian et al 2016).…”

Section: Preparation Of Crude Extractmentioning

confidence: 99%

Application of Soil‐borne Actinomycetes for Biological Control against Fusarium Wilt of Chickpea (Cicer arietinum) caused by Fusarium solani fsp pisi

Soltanzadeh

Nejad

Bonjar

2016

Journal of Phytopathology

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Black root rot, caused by Fusarium solani f.sp. pisi, is a devastating soil‐borne disease in chickpea in Iran with no effective control measures. With the aim of finding applicable biocontrol agents to alleviate the malady, isolates of Actinomycetes isolated from soil and their antagonistic effect against F. solani f.sp. pisi were evaluated both in vitro and in vivo. More than 100 Actinomycetes isolates were screened for their antifungal activities against the pathogen. The most active isolates were evaluated in greenhouse for their biocontrol performance. Based on the results of dual cultures in screening evaluations, the size of inhibition zone of fungal growth, and the most effective antagonist isolates (S3, S12 and S40) were selected for further studies. Identity of active isolates was determined, in this regard, 16S rDNA of isolates were amplified using universal bacterial primers FD1 and RP2. The PCR products were purified and sequenced. Sequence analysis of 16S rDNA was then performed using NCBI BLAST method. Comparison of the near full length 16S rRNA sequence of isolates to GenBank sequences demonstrated that isolates S3 and S12 were most similar to Streptomyces antibioticus, while isolate S40 was most similar to Streptomyces peruviensis. Biocontrol studies of these isolates in control of the disease in greenhouse significantly decreased the disease severity. Actinomycetes isolate S12 demonstrated the greatest effect in reducing disease than the other two. Results of this research are at preliminary stage for developing biocontrol agents. These data can be utilized as a platform for future studies with the aim of commercializing these biocontrol products and hoping to step towards sustainable agriculture.

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Biocontrol of Botrytis allii Munn the Causal Agent of Neck Rot, the Post Harvest Disease in Onion, by Use of a New Iranian Isolate of Streptomyces

Cited by 9 publications

References 4 publications

Assessment of the antifungal activities of polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant against fungi isolated from papaya (Carica papaya L.) fruit

Assessment of the antifungal activities of polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant against fungi isolated from papaya (Carica papaya L.) fruit

Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil

Application of Soil‐borne Actinomycetes for Biological Control against Fusarium Wilt of Chickpea (Cicer arietinum) caused by Fusarium solani fsp pisi

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