Biocontrol of Anthracnose Disease on Chili Pepper Using a Formulation Containing Paenibacillus polymyxa C1

Suprapta, Dewa Ngurah

doi:10.3389/fsufs.2021.782425

Cited by 8 publications

(3 citation statements)

References 20 publications

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“…On the other hand, the GYUN-2311 treatment has shown the disease rate of 36.18% which is similar to TK ® treatment with disease rate of 37.52%. In support of our findings, Suprapta (2022) provided evidence that the application of Paenibacillus polymyxa C1 successfully reduced the occurrence of anthracnose incidence in chili peppers under greenhouse conditions by 30.38–97.34% subsequent to treatment with different formulations, as compared to the control group ( p < 0.05). Several Bacillus species have been reported to reduce the severity of disease on a range of hosts ( Kim et al, 2012 ; Lee et al, 2013 ).…”

Section: Discussionsupporting

confidence: 86%

Characterization and evaluation of Bacillus subtilis GYUN-2311 as a biocontrol agent against Colletotrichum spp. on apple and hot pepper in Korea

Heo,

Lee,

Balaraju

et al. 2024

Front. Microbiol.

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Crop plants are vulnerable to a variety of diseases, including anthracnose, caused by various species of Colletotrichum fungi that damages major crops, including apples and hot peppers. The use of chemical fungicides for pathogen control may lead to environmental pollution and disease resistance. Therefore, we conducted this research to develop a Bacillus subtilis-based biological control agent (BCA). B. subtilis GYUN-2311 (GYUN-2311), isolated from the rhizosphere soil of an apple orchard, exhibited antagonistic activity against a total of 12 fungal pathogens, including eight Colletotrichum species. The volatile organic compounds (VOCs) and culture filtrate (CF) from GYUN-2311 displayed antifungal activity against all 12 pathogens, with 81% control efficiency against Fusarium oxysporum for VOCs and 81.4% control efficacy against Botryosphaeria dothidea for CF. CF also inhibited germination and appressorium formation in Colletotrichum siamense and C. acutatum. The CF from GYUN-2311 showed antifungal activity against all 12 pathogens in different media, particularly in LB medium. It also exhibited plant growth-promoting (PGP) activity, lytic enzyme activity, siderophore production, and the ability to solubilize insoluble phosphate. In trials on apples and hot peppers, GYUN-2311 effectively controlled disease, with 75 and 70% control efficacies against C. siamense in wounded and unwounded apples, respectively. Similarly, the control efficacy of hot pepper against C. acutatum in wounded inoculation was 72%. Combined application of GYUN-2311 and chemical suppressed hot pepper anthracnose to a larger extent than other treatments, such as chemical control, pyraclostrobin, TK®, GYUN-2311 and cross-spraying of chemical and GYUN-2311 under field conditions. The genome analysis of GYUN-2311 identified a circular chromosome comprising 4,043 predicted protein-coding sequences (CDSs) and 4,096,969 bp. B. subtilis SRCM104005 was the strain with the highest average nucleotide identity (ANI) to GYUN-2311. AntiSMASH analysis identified secondary metabolite biosynthetic genes, such as subtilomycin, bacillaene, fengycin, bacillibactin, pulcherriminic acid, subtilosin A, and bacilysin, whereas BAGEL analysis confirmed the presence of competence (ComX). Six secondary metabolite biosynthetic genes were induced during dual culture in the presence of C. siamense. These findings demonstrate the biological control potential of GYUN-2311 against apple and hot pepper anthracnose.

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Section: Discussionsupporting

confidence: 86%

Characterization and evaluation of Bacillus subtilis GYUN-2311 as a biocontrol agent against Colletotrichum spp. on apple and hot pepper in Korea

Heo,

Lee,

Balaraju

et al. 2024

Front. Microbiol.

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“…Similarly, the mushroom culture filtrate, Clitocybe nuda (LA82), effectively reduced the severity of anthracnose disease (Chen and Huang, 2010). Suprapta (2022) found that the formulation of Paenibacillus polymyxa C1 effectively controlled the anthracnose disease under field conditions.…”

Section: Effect Of Antagonistic Activity On C Capsici Severity Determ...mentioning

confidence: 97%

Biological Control of Chili Anthracnose Disease using Talaromyces flavus Bodhi001

Oiuphisittraiwat,

Dethoup,

Jantasorn

2023

IJARe

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Background: Anthracnose disease of chili caused by Colletotrichum spp is one of the most destructive diseases affecting chili fruits in Thailand and significantly reduces fruit quality and chili production. Currently, this disease is managed primarily with synthetic fungicides that may affect public health and the environment adversely. Consequently, there is a need for biological management options. In vitro and in vivo evaluations of the antagonistic activity of Talaromyces flavus Bodhi001, Talaromyces trachyspermus Bodhi002, Talaromyces flavus Bodhi003 and Neosartorya fischeri Bodhi004 against Colletotrichum capsici, the causal agent of chili anthracnose disease, were conducted in the present study. Methods: The activity of antagonistic fungi against C. capsici was determined using PDA plate by dual culture method. The spore suspensions of C. capsici and antagonistic fungi were prepared in sterile water and adjusted using a hemocytometer to achieve a final concentration of about 106 spores mL-1. Result: The most effective antagonistic strains were T. flavus Bodhi001 and N. fischeri Bodhi004, which inhibited the mycelial growth of C. capsici by 68.99% and 70.76%, respectively. Interestingly, the antagonistic T. flavus Bodhi001 strain was the most effective at reducing the severity of chili anthracnose in vivo by up to 80%. The biological control activity of T. flavus Bodhi001 was to produce antibiosis against C. capsici; therefore, testing can be recommended to confirm its field trial stability. The results indicate that the application of the antagonistic fungi T. flavus Bodhi001 may be quite effective in biological control of chili anthracnose.

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“…The bacterial isolates were transferred with an ose needle to the PDA media and incubated at 27 ± 2 • C for 3 days. Fermentation (room temperature 28-30 • C ) of P. caninum leafs extract: a mixture of 20 mg of P caninum extract, 1 one liter of potato boiled water, and 1 ml suspension of B. agri was transferred into a glass bottle and incubated for 30 days at 27 ± 2 • C) before application to rice plants (Suprapta, 2022).…”

Section: Production Of B Agrimentioning

confidence: 99%

Piper caninum extract and Brevibacillus agri mixture suppresses rice leaf spot pathogen; Nigrospora oryzae and improves the production of red rice (Oryza sativa L)

Suriani

Suprapta

Suarsana

et al. 2022

Front. Sustain. Food Syst.

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Under the guise of enhancing productivity, using pesticides and artificial fertilizers in agriculture affects both the environment and living things. High chemical residues in food and the environment disrupt the health of consumers. One of the solutions that can bring about a reduction in the use of pesticides and chemicals is switching to organic fertilizers. The application of biopesticides originating from biological sources such as plant extracts and the use of microbes is gaining global acceptance. Therefore, this study aimed to obtain the best biopesticides and biostimulants that could suppress the leaf spot pathogen, Nigrospora oryzae, and increase the growth and yield of Bali red rice. The study contained four treatments, namely untreated control (F0), Piper caninum leaf extract (F1), Brevibacillus agri (F2), and fermented P. caninum leaf extract plus B. agri (F3). The treatments were arranged in a randomized complete block design, and each treatment was replicated three times. The parameters measured were the number of tillers per plant, number of leafs per plant, chlorophyll content, number of grains per panicle, grain weight, and grain yield. Furthermore, antimicrobial and antioxidants were assayed using SEM. GC-MS. At the end of the experiment, the disease index of the leaf spot was measured. The results showed that F3 significantly suppressed leaf spots caused by N. oryzae compared to other treatments, including untreated control in red rice. Additionally, the F3 significantly increased the number of productive tillers, number of grains per panicle, and grain yield compared to all other treatments. The F3 enhanced the crop yield at 6.19 tons/ha, an increase of 50% compared to the untreated control. The SEM.GC-MS results showed the presence of 2.3 butanediol, tetra-decanoic acid, butanoic acid, ethyl ester, benzene propanal, 3-(1,1-dimethylethyl)-a-methyl, a-N-Normethadol in treated plants with P. canicum plus B. agri.

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Biocontrol of Anthracnose Disease on Chili Pepper Using a Formulation Containing Paenibacillus polymyxa C1

Cited by 8 publications

References 20 publications

Characterization and evaluation of Bacillus subtilis GYUN-2311 as a biocontrol agent against Colletotrichum spp. on apple and hot pepper in Korea

Characterization and evaluation of Bacillus subtilis GYUN-2311 as a biocontrol agent against Colletotrichum spp. on apple and hot pepper in Korea

Biological Control of Chili Anthracnose Disease using Talaromyces flavus Bodhi001

Piper caninum extract and Brevibacillus agri mixture suppresses rice leaf spot pathogen; Nigrospora oryzae and improves the production of red rice (Oryza sativa L)

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