Biocontrol Ability of the Bacillus amyloliquefaciens Group, B. amyloliquefaciens, B. velezensis, B. nakamurai, and B. siamensis, for the Management of Fungal Postharvest Diseases: A Review

Wang, Suyan; Herrera‐Balandrano, Daniela D.; Wang, Yanxia; Shi, Xin-Chi; Chen, Xin; Jin, Yingji; Liu, Feng-Quan; Laborda, Pedro

doi:10.1021/acs.jafc.2c01745

Cited by 46 publications

(20 citation statements)

References 164 publications

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“…Fusarium solani causes rot in a wide variety of crops, including citrus, rice, peas, beans and potatos. Pathogenic fungi can be combated with biocontrol strategies rather than fungicide, which is less harmful to ecosystems [ 45 , 46 , 47 ]. The antifungal activity of the Penicillium chrysogenum protein has been stated by [ 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Acetylcholine Esterase Inhibitory Effect, Antimicrobial, Antioxidant, Metabolomic Profiling, and an In Silico Study of Non-Polar Extract of The Halotolerant Marine Fungus Penicillium chrysogenum MZ945518

et al. 2023

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Major health issues, such as the rise in oxidative stress, incidences of Alzheimer’s disease, and infections caused by antibiotic-resistant microbes, have prompted researchers to look for new therapeutics. Microbial extracts are still a good source of novel compounds for biotechnological use. The objective of the current work was to investigate marine fungal bioactive compounds with potential antibacterial, antioxidant, and acetylcholinesterase inhibitory effects. Penicillium chrysogenum strain MZ945518 was isolated from the Mediterranean Sea in Egypt. The fungus was halotolerant with a salt tolerance index of 1.3. The mycelial extract showed antifungal properties against Fusarium solani with an inhibitory percentage of 77.5 ± 0.3, followed by Rhizoctonia solani and Fusarium oxysporum with percentages of 52 ± 0.0 and 40 ± 0.5, respectively. The extract also showed antibacterial activity against both Gram-negative and Gram-positive bacterial strains using the agar diffusion technique. The fungal extract was significantly more effective with Proteus mirabilis ATCC 29906 and Micrococcus luteus ATCC 9341; inhibition zones recorded 20 and 12 mm, respectively, compared with the antibiotic gentamycin, which recorded 12 and 10 mm, respectively. The antioxidant activity of the fungus extract revealed that it successfully scavenged DPPH free radicals and recorded an IC50 of 542.5 µg/mL. Additionally, it was capable of reducing Fe3+ to Fe2+ and exhibiting chelating ability in the metal ion-chelating test. The fungal extract was identified as a crucial inhibitor of acetylcholinesterase with an inhibition percentage of 63% and an IC50 value of 60.87 µg/mL. Using gas chromatography–mass spectrometry (GC/MS), 20 metabolites were detected. The most prevalent ones were (Z)-18-octadec-9-enolide and 1,2-Benzenedicarboxylic acid, with ratios of 36.28 and 26.73%, respectively. An in silico study using molecular docking demonstrated interactions between the major metabolites and the target proteins, including: DNA Gyrase, glutathione S-transferase, and Acetylcholinesterase, confirming the extract’s antimicrobial and antioxidant activity. Penicillium chrysogenum MZ945518, a halotolerant strain, has promising bioactive compounds with antibacterial, antioxidant, and acetylcholinesterase inhibitory activities

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Section: Discussionmentioning

confidence: 99%

Acetylcholine Esterase Inhibitory Effect, Antimicrobial, Antioxidant, Metabolomic Profiling, and an In Silico Study of Non-Polar Extract of The Halotolerant Marine Fungus Penicillium chrysogenum MZ945518

et al. 2023

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“…Generally, antifungal mechanisms of Bacillus species for the control of postharvest fungal diseases include the secretion of antifungal metabolites and lytic enzymes, volatile organic compounds, competition for nutrients, and the induced resistance [41]. The biocontrol strains B. amyloliquefaciens DH‐4, J4F, J4D, G3A2, J4FS, G3AM1 MG3, B10W10, and PG12, the strains B. velezensis XT1 CECT 8661 and SL‐6 were reported to produce antifungal metabolites including polyenes, bacillaene, and macrolactin, or cyclic lipopeptides [41]. The control effects of B. amyloliquefaciens CPA‐8, BUZ‐14, I3, LI24 and PP19, Bacillus siamensis G‐3, B. velezensis Zsy‐1, B. licheniformis HS10, and B. pumilus PI26 against postharvest diseases were ascribed to the secretion of volatile compounds [41].…”

Section: Discussionmentioning

confidence: 99%

“…To some extent, B. velezensis FJAT-52631 performed a comparative biocontrol effect with the strains B. amyloliquefaciens HG01 and B4. Generally, antifungal mechanisms of Bacillus species for the control of postharvest fungal diseases include the secretion of antifungal metabolites and lytic enzymes, volatile organic compounds, competition for nutrients, and the induced resistance [41]. The biocontrol strains B. amyloliquefaciens DH-4, J4F, J4D, G3A2, J4FS, G3AM1 MG3, B10W10, and PG12, the strains B. velezensis XT1 CECT 8661 and SL-6 were reported to produce antifungal metabolites including polyenes, bacillaene, and macrolactin, or cyclic lipopeptides [41].…”

Section: Effects Of the Purified Lipopeptides Against C Acutatum Ex Situmentioning

confidence: 99%

Antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against anthracnose pathogen Colletotrichum acutatum

et al. 2023

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This study was aim at investigating antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against Colletotrichum acutatum ex situ and in situ. The results showed that the strain FJAT‐52631 and its crude lipopeptides (10 mg/ml) exhibited strong inhibitory effects on growth of C. acutatum FJAT‐30256 with an inhibition rate of 75.3% and an inhibition zone diameter of 17.66 mm, respectively. Both the viable bacterial cultures and lipopeptides of FJAT‐52631 could delay the onset of loquat anthracnose by 1 day and lower the incidence of loquat anthracnose in situ. The whole cultures of B. velezensis FJAT‐52631 displayed a 50% biocontrol efficacy on loquat anthracnose at the fourth day after inoculation, but the crude lipopeptides not. The average lesion diameter of the whole‐culture treated group was 5.62 mm, which was smaller than that of control group (6.81 mm). All the three types of lipopeptides including iturin A, fengycin, and surfactin A secreted from the strain FJAT‐52631 exhibited antifungal activities. Among them, surfactin A displayed higher antifungal activity at a concentration of 1.25 mg/mL than other two lipopeptides even if at a concentration of 60 mg/mL. Thus, the results indicated that surfactin A produced by FJAT‐52631 played a major role in the biocontrol of the loquat anthracnose. Scanning electron microscopy (SEM) observation revealed the structural deformities in the mycelia of C. acutatum. The above results suggested that the antifungal lipopeptides from B. velezensis FJAT‐52631 would be potential in biocontrol against anthracnose disease of loquat caused by C. acutatum.

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“…The Bacillus genus groups multiple species that have a wide diversity of mechanisms to inhibit the growth of pathogens such as B. cinerea . For example, the Bacillus amyloliquefaciens group, which includes species such as B. amyloliquefaciens , B. velezensis , B. nakamurai , and B. siamensis , has been very effective in postharvest action to reduce the harmful effects of Botrytis , among other fungal pathogens [ 93 ].…”

Section: Antagonistic Effects Of Pgpb On Botrytis Cinereamentioning

confidence: 99%

Agroecological Management of the Grey Mould Fungus Botrytis cinerea by Plant Growth-Promoting Bacteria

Orozco-Mosqueda

Kumar

Fadiji

et al. 2023

Plants

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Botrytis cinerea is the causal agent of grey mould and one of the most important plant pathogens in the world because of the damage it causes to fruits and vegetables. Although the application of botrycides is one of the most common plant protection strategies used in the world, the application of plant-beneficial bacteria might replace botrycides facilitating agroecological production practices. Based on this, we reviewed the different stages of B. cinerea infection in plants and the biocontrol mechanisms exerted by plant-beneficial bacteria, including the well-known plant growth-promoting bacteria (PGPB). Some PGPB mechanisms to control grey mould disease include antibiosis, space occupation, nutrient uptake, ethylene modulation, and the induction of plant defence mechanisms. In addition, recent studies on the action of anti-Botrytis compounds produced by PGPB and how they damage the conidial and mycelial structures of the pathogen are reviewed. Likewise, the advantages of individual inoculations of PGPB versus those that require the joint action of antagonist agents (microbial consortia) are discussed. Finally, it should be emphasised that PGPB are an excellent option to prevent grey mould in different crops and their use should be expanded for environmentally friendly agricultural practices.

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Biocontrol Ability of the Bacillus amyloliquefaciens Group, B. amyloliquefaciens, B. velezensis, B. nakamurai, and B. siamensis, for the Management of Fungal Postharvest Diseases: A Review

Cited by 46 publications

References 164 publications

Acetylcholine Esterase Inhibitory Effect, Antimicrobial, Antioxidant, Metabolomic Profiling, and an In Silico Study of Non-Polar Extract of The Halotolerant Marine Fungus Penicillium chrysogenum MZ945518

Acetylcholine Esterase Inhibitory Effect, Antimicrobial, Antioxidant, Metabolomic Profiling, and an In Silico Study of Non-Polar Extract of The Halotolerant Marine Fungus Penicillium chrysogenum MZ945518

Antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against anthracnose pathogen Colletotrichum acutatum

Agroecological Management of the Grey Mould Fungus Botrytis cinerea by Plant Growth-Promoting Bacteria

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