Biocomposite scaffolds containing regenerated silk fibroin and nanohydroxyapatite for bone tissue regeneration

Агапов, И. И.; Moisenovich, Mikhail M.; Druzhinina, T. V.; Kamenchuk, Ya. A.; Trofimov, K. V.; Vasilyeva, T. V.; Kon’kov, A. S.; Arhipova, A. Yu.; Sokolova, Olga S.; Guzeev, V; Kirpichnikov, Mikhaǐl P.

doi:10.1134/s1607672911050103

Cited by 4 publications

(6 citation statements)

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“…We had previously formed silk fibroin scaffolds [6] and silk fibroin–HA scaffolds [5], and examined the biological properties of the pilot samples. The scaffolds possess all the characteristics needed for bone surgery; in particular, they are biocompatible, strong, and porous.…”

Section: Resultsmentioning

confidence: 99%

“…Pores connected with holes and channels form a complex, unclosed internal surface that facilitates cell migration to the internal layers of an artificial scaffold. Furthermore, an unclosed pore structure provides conditions for the medium exchange and removal of metabolites, thus facilitating the formation of a homogenous intra-scaffold medium [5, 7-9]. …”

Section: Resultsmentioning

confidence: 99%

“…It preserves its functional characteristics for a given period, causes no local inflammatory response, does not trigger the spread of an infection, and is replaced with a patient’s native tissue over time; therefore, it is a material suitable for bone tissue re-generation [5-7]. …”

Section: Resultsmentioning

confidence: 99%

“…Due to this parameter, it is soluble neither in water nor in the diluted solutions of some acids and bases [13], while it is negatively charged at physiological pH=7, in contrast to the positively charged spidroin [5], thus decreasing cell adhesion and increasing the cell proliferation rate [5]. …”

Section: Resultsmentioning

confidence: 99%

“…The reduced capability of fibroin materials to maintain cell adhesion and proliferation has the potential to cause a poorer re-generation ability compared with that of spidroin scaffolds in experiments with a bone injury model. The re-generative properties of fibroin scaffolds in these experiments were considerably improved by the use of nano-hydroxyapatite mineralization [5]. We have introduced a combination of two composite additives, nano-hydroxyapatite (a bone tissue component) and gelatin (a collagen derivative), into the formulations of fibroin scaffolds to enhance their capability to maintain the adhesion and proliferation of fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

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Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

et al. 2014

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Three-dimensional (3D) silk fibroin scaffolds were modified with one of the major bone tissue derivatives (nano-hydroxyapatite) and/or a collagen derivative (gelatin). Adhesion and proliferation of mouse embryonic fibroblasts (MEF) within the scaffold were increased after modification with either nano-hydroxyapatite or gelatin. However, a significant increase in MEF adhesion and proliferation was observed when both additives were introduced into the scaffold. Such modified composite scaffolds provide a new and better platform to study wound healing, bone and other tissue regeneration, as well as artificial organ bioengineering. This system can further be applied to establish experimental models to study cell-substrate interactions, cell migration and other complex processes, which may be difficult to address using the conventional two-dimensional culture systems.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

et al. 2014

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Orange IV stabilizes silk fibroin microemulsions

Ferreira

Volkov

Abreu

et al. 2015

Engineering in Life Sciences

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Silk fibroin (SF) is a natural biopolymer that has been extensively studied in various applications due to its impressive mechanical properties and biocompatibility. Recently, SF‐based particles have been proposed as controlled drug delivery systems. A new and efficient method to prepare SF microemulsions (SF‐MEs) was developed by oil‐in‐water emulsions using high‐pressure homogenization to promote emulsification. During SF‐ME production, the secondary structure of SF changed to a more stable conformation (from random coil to β‐sheets), thus allowing the formation of small and stable (140.7 ± 1.9 nm; polydispersity index, 0.25) SF microparticles (SF‐MPs). The efficiency of SF‐MP formation was 60%. Orange IV was used as a model compound for incorporation and release studies, although its incorporation into the SF‐MEs significantly improved particle size and size distribution over at least 4 wk compared to traditional stabilizers (e.g., poloxamer 407, transcutol, Tween 80, and SDS). This should be a call of attention when using dyes as model compounds since they can influence particle properties and lead to misinterpretation of the results. Orange IV showed an incorporation efficiency of 91% and a controlled release over time. Stable SF‐MP formulations, further enhanced by orange IV incorporation, provide an innovative method with potential application in pharmaceutical development due to its associated high biocompatibility and release profile.

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Relationship between Gelatin Concentrations in Silk Fibroin-Based Composite Scaffolds and Adhesion and Proliferation of Mouse Embryo Fibroblasts

et al. 2014

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Porous scaffolds of silk fibroin and composite porous scaffolds with 10, 20, 30, 40, and 50% gelatin were made by the freezing-thawing method. The relationship between adhesion and proliferation rate mouse embryo fibroblast and the scaffold composition was studied by laser confocal scanning microscopy. Addition of gelatin to the scaffold structure stimulated adhesion and proliferation of mouse embryo fibroblasts; the optimal content of gelatin was 30%.

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Biocomposite scaffolds containing regenerated silk fibroin and nanohydroxyapatite for bone tissue regeneration

Cited by 4 publications

References 4 publications

Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

Orange IV stabilizes silk fibroin microemulsions

Relationship between Gelatin Concentrations in Silk Fibroin-Based Composite Scaffolds and Adhesion and Proliferation of Mouse Embryo Fibroblasts

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