Biocompatible superparamagnetic iron oxide nanoparticles used for contrast agents: a structural and magnetic study

Gamarra, Lionel Fernel; Brito, G.E.S.; Pontuschka, W. M.; Amaro, Edson; Parma, Alvise; Goya, Gerardo F.

doi:10.1016/j.jmmm.2004.11.123

Cited by 97 publications

(61 citation statements)

References 5 publications

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“…Dextran-coated SPIONs have been shown to accumulate in various types of cells, including cancer cells, and have been used in clinical settings for a long time. [38][39][40][41] This process can be quick; that is, labeling takes only a few minutes. The nanoparticles were internalized depending on tumor cell size and morphology, which led to widespread heterogeneity.…”

mentioning

confidence: 99%

Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells

Lindemann¹,

Lüdtke‐Buzug²,

Fräderich³

et al. 2014

IJN

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Background As a tomographic imaging technology, magnetic particle imaging (MPI) allows high spatial resolution and sensitivity, and the possibility to create real-time images by determining the spatial distribution of magnetic particles. To ensure a prospective biosafe application of UL-D (University of Luebeck-Dextran coated superparamagnetic nanoparticles), we evaluated the biocompatibility of superparamagnetic iron oxide nanoparticles (SPIONs), their impact on biological properties, and their cellular uptake using head and neck squamous cancer cells (HNSCCs). Methods SPIONs that met specific MPI requirements were synthesized as tracers. Labeling and uptake efficiency were analyzed by hematoxylin and eosin staining and magnetic particle spectrometry. Flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays, and real-time cell analyzer assays were used to investigate apoptosis, proliferation, and the cytokine response of SPION-labeled cells. The production of reactive oxygen species (ROS) was determined using a fluorescent dye. Experimental results were compared to the contrast agent Resovist ® , a standard agent used in MPI. Results UL-D nanoparticles and Resovist particles were taken up in vitro by HNSCCs via unspecific phagocytosis followed by cytosolic accumulation. To evaluate toxicity, flow cytometry analysis was performed; results showed that dose- and time-dependent administration of Resovist induced apoptosis whereas cell viability of UL-D-labeled cells was not altered. We observed decreased cell proliferation in response to increased SPION concentrations. An intracellular production of ROS could not be detected, suggesting that the particles did not cause oxidative stress. Tumor necrosis factor alpha (TNF-α) and interleukins IL-6, IL-8, and IL-1β were measured to distinguish inflammatory responses. Only the primary tumor cell line labeled with >0.5 mM Resovist showed a significant increase in IL-1β secretion. Conclusion Our data suggest that UL-D SPIONs are a promising tracer material for use in innovative tumor cell analysis in MPI.

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mentioning

confidence: 99%

Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells

Lindemann¹,

Lüdtke‐Buzug²,

Fräderich³

et al. 2014

IJN

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“…Since this reaction occurs at room temperature, thermic treatments are required in order to obtain the final crystalline structure [62,63]. Gamarra et al [64] precipitated iron oxyhydroxide (FeOOH) in water in the presence of a surfactant, after which they reduced the Fe 3+ at Fe 2+ partially, by a light drying in N 2 atmosphere, obtaining at the end magnetite particles.…”

Section: Sol-gel Methodsmentioning

confidence: 99%

Pros and Cons on Magnetic Nanoparticles Use in Biomedicine and Biotechnologies Applications

Bojin

Păunescu

2014

Nanoparticles' Promises and Risks

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In recent years, the design and synthesis of colloidal magnetic suspensions have attracted an increased interest especially in the fields of biotechnology and biomedicine because they have many applications including targeted drug delivery, cell labeling and magnetic cell separation, hyperthermia, tissue repairing, magnetic resonance imaging (MRI) contrast enhancement, enzyme immobilization, immunoassays, protein purification, etc. IntroductionMagnetic nanoparticles (MNPs) used in biomedicine must meet several requirements. They have to be non-toxic, chemically stabile, uniform in size, well stabilized under physiological conditions, biocompatible and to present high magnetization. Magnetite (Fe 3 O 4 ) and maghemite (γ-Fe 2 O 3 ) are the most suitable iron oxide nanoparticles employed for biomedical applications because they are biocompatible, have low toxicity in the human body and show a superparamagnetic behavior.Therefore, synthesis of magnetic iron oxide nanoparticles with tailored properties has attracted considerable scientific and technological interest. Various synthesis routes were developed for producing magnetic particles, such as co-precipitation, microemulsion method, thermal decomposition of different organic precursors, spray pyrolysis, and sol-gel method.For biomedical applications, magnetic iron oxide nanoparticles must be dispersed in biocompatible media in order to obtain stable colloidal suspensions. In order to prevent the particle aggregation and to improve the biocompatibility and stability, nanoparticles are coated with various surfactants: poly(ethylene glycol),

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“…Magnetite-based ferrofluids were synthesized through a modified sol-gel method (Gamarra et al, 2005). They precipitated iron oxyhydroxy in water in the presence of a surfactant (Renex), and next they partiallly reduced Fe 3+ to Fe 2+ by a mild drying process in N 2 atmosphere, leading to the formation of magnetite.…”

Section: Sol-gel Reactionmentioning

confidence: 99%

Tailored and Functionalized Magnetite Particles for Biomedical and Industrial Applications

Durdureanu-Angheluta¹,

Pinteală²,

Simionescu³

2012

Materials Science and Technology

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Biocompatible superparamagnetic iron oxide nanoparticles used for contrast agents: a structural and magnetic study

Cited by 97 publications

References 5 publications

Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells

Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells

Pros and Cons on Magnetic Nanoparticles Use in Biomedicine and Biotechnologies Applications

Tailored and Functionalized Magnetite Particles for Biomedical and Industrial Applications

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