Biocompatible MoS2/PDA-RGD coating on titanium implant with antibacterial property via intrinsic ROS-independent oxidative stress and NIR irradiation

Yuan, Zhangfu; Tao, Bailong; He, Ye; Liu, Ju; Lin, Changjian; Shen, Xinkun; Ding, Yao; Yu, Yu; Mu, Caiyun; Liu, Peng; Cai, Kun

doi:10.1016/j.biomaterials.2019.119290

Cited by 188 publications

(114 citation statements)

References 64 publications

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“…Therefore, the combination of PTA with PDA to prevent bacterial infection has attracted more attention and in fact shown promising antibacterial effects [ 19 , 20 ]. However, the long-term safety of the common photocatalytic and photothermal materials such as carbon-based nanomaterials [ 21 ], black phosphorus [ 22 ], red phosphorus [ 19 ], MoS 2 [ [23] , [24] , [25] ], gold nanoparticles [ 26 ], and copper sulfide [ 27 ] remains to be seen in vivo .…”

Section: Introductionmentioning

confidence: 99%

Synergistic antibacterial activity of physical-chemical multi-mechanism by TiO2 nanorod arrays for safe biofilm eradication on implant

Zhang

Chai

et al. 2021

Bioactive Materials

125

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Treatment of implant-associated infection is becoming more challenging, especially when bacterial biofilms form on the surface of the implants. Developing multi-mechanism antibacterial methods to combat bacterial biofilm infections by the synergistic effects are superior to those based on single modality due to avoiding the adverse effects arising from the latter. In this work, TiO 2 nanorod arrays in combination with irradiation with 808 near-infrared (NIR) light are proven to eradicate single specie biofilms by combining photothermal therapy, photodynamic therapy, and physical killing of bacteria. The TiO 2 nanorod arrays possess efficient photothermal conversion ability and produce a small amount of reactive oxygen species (ROS). Physiologically, the combined actions of hyperthermia, ROS, and puncturing by nanorods give rise to excellent antibacterial properties on titanium requiring irradiation for only 15 min as demonstrated by our experiments conducted in vitro and in vivo . More importantly, bone biofilm infection is successfully treated efficiently by the synergistic antibacterial effects and at the same time, the TiO 2 nanorod arrays improve the new bone formation around implants. In this protocol, besides the biocompatible TiO 2 nanorod arrays, an extra photosensitizer is not needed and no other ions would be released. Our findings reveal a rapid bacteria-killing method based on the multiple synergetic antibacterial modalities with high biosafety that can be implemented in vivo and obviate the need for a second operation. The concept and antibacterial system described here have large clinical potential in orthopedic and dental applications.

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Section: Introductionmentioning

confidence: 99%

Synergistic antibacterial activity of physical-chemical multi-mechanism by TiO2 nanorod arrays for safe biofilm eradication on implant

Zhang

Chai

et al. 2021

Bioactive Materials

125

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“…In recent years, a number of studies aimed at enhancing osseointegration and antibacterial properties have been carried out. For example, the MoS 2 /PDA-RGD coating on titanium implants can not only promote the integration of a titanium implant with the host bone but also inhibit bacterial growth with a high efficiency [6]. In addition, the surface topography has attracted more and more attention, and topographical modification differs from chemical modifications by only changing the micro-and nano-scale structure.…”

Section: Introductionmentioning

confidence: 99%

F-actin Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through MKL1 and YAP/TAZ

et al. 2020

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Titanium and titanium alloys are widely used in orthopedic implants. Modifying the nanotopography provides a new strategy to improve osseointegration of titanium substrates. Filamentous actin (F-actin) polymerization, as a mechanical loading structure, is generally considered to be involved in cell migration, endocytosis, cell division, and cell shape maintenance. Whether F-actin is involved and how it functions in nanotube-induced osteogenic differentiation of mesenchymal stem cells (MSCs) remain to be elucidated. In this study, we fabricated TiO2 nanotubes on the surface of a titanium substrate by anodic oxidation and characterized their features by scanning electron microscopy (SEM), X-ray energy dispersive analysis (EDS), and atomic force microscopy (AFM). Alkaline phosphatase (ALP) staining, Western blotting, qRT-PCR, and immunofluorescence staining were performed to explore the osteogenic potential, the level of F-actin, and the expression of MKL1 and YAP/TAZ. Our results showed that the inner diameter and roughness of TiO2 nanotubes increased with the increase of the anodic oxidation voltage from 30 to 70 V, while their height was 2 μm consistently. Further, the larger the tube diameter, the stronger the ability of TiO2 nanotubes to promote osteogenic differentiation of MSCs. Inhibiting F-actin polymerization by Cyto D inhibited osteogenic differentiation of MSCs as well as the expression of proteins contained in focal adhesion complexes such as vinculin (VCL) and focal adhesion kinase (FAK). In contrast, after Jasp treatment, polymerization of F-actin enhanced the expression of RhoA and transcription factors YAP/TAZ. Based on these data, we concluded that TiO2 nanotubes facilitated the osteogenic differentiation of MSCs, and this ability was enhanced with the increasing diameter of the nanotubes within a certain range (30–70 V). F-actin mediated this process through MKL1 and YAP/TAZ.

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“…For example, mussel-inspired PDA is emerging as an excellent photothermal agent because of its good biocompatibility and efficient NIR light-responsive photothermal conversion. It has been reported that photosensitizer-loaded mesoporous PDA nanoparticles, as well as MoS 2 /PDA-arginine-glycine-aspartic acid (RGD) decorations of bone implants, displayed synergistic photothermal and photodynamic effects for antibacterial therapy 16 , 17 . Similarly, a hybrid BS@Ag 3 PO 4 coating on bone implants also exhibited good photothermal effects using BS as photocatalyst 90 .…”

Section: Discussionmentioning

confidence: 99%

NIR light-assisted phototherapies for bone-related diseases and bone tissue regeneration: A systematic review

Wan¹,

Zhang²,

Lv³

et al. 2020

Theranostics

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Recently, the rapid development of biomaterials has induced great interest in the precisely targeted treatment of bone-related diseases, including bone cancers, infections, and inflammation. Realizing noninvasive therapeutic effects, as well as improving bone tissue regeneration, is essential for the success of bone‑related disease therapies. In recent years, researchers have focused on the development of stimuli-responsive strategies to treat bone-related diseases and to realize bone regeneration. Among the various external stimuli for targeted therapy, near infrared (NIR) light has attracted considerable interests due to its high tissue penetration capacity, minimal damage toward normal tissues, and easy remote control properties. The main objective of this systematic review was to reveal the current applications of NIR light-assisted phototherapy for bone-related disease treatment and bone tissue regeneration. Database collection was completed by June 1, 2020, and a total of 81 relevant studies were finally included. We outlined the various therapeutic applications of photothermal, photodynamic and photobiomodulation effects under NIR light irradiation for bone‑related disease treatment and bone regeneration, based on the retrieved literatures. In addition, the advantages and promising applications of NIR light-responsive drug delivery systems for spatiotemporal-controlled therapy were summarized. These findings have revealed that NIR light-assisted phototherapy plays an important role in bone-related disease treatment and bone tissue regeneration, with significant promise for further biomedical and clinical applications.

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Biocompatible MoS2/PDA-RGD coating on titanium implant with antibacterial property via intrinsic ROS-independent oxidative stress and NIR irradiation

Cited by 188 publications

References 64 publications

Synergistic antibacterial activity of physical-chemical multi-mechanism by TiO2 nanorod arrays for safe biofilm eradication on implant

Synergistic antibacterial activity of physical-chemical multi-mechanism by TiO2 nanorod arrays for safe biofilm eradication on implant

F-actin Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through MKL1 and YAP/TAZ

NIR light-assisted phototherapies for bone-related diseases and bone tissue regeneration: A systematic review

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