Biocompatible Magnetic Colloidal Suspension Used as a Tool for Localized Hyperthermia in Human Breast Adenocarcinoma Cells: Physicochemical Analysis and Complex In Vitro Biological Profile

Moacă, Elena-Alina; Watz, Claudia-Geanina; Socoliuc, Vlad; Racoviceanu, Roxana; Păcurariu, Cornelia; Ianoş, Robert; Pinzaru, Simona Cîntă; Tudoran, Lucian Barbu; Nekvapil, Fran; Iurciuc, Stela; Şoica, Codruţa; Dehelean, Cristina-Adriana

doi:10.3390/nano11051189

Cited by 13 publications

(9 citation statements)

References 76 publications

(84 reference statements)

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“…The Fe 3 O 4 @C aqueous suspension was highly negatively charged (−47.48 mV) with a narrow size distribution and a single family of nanoparticles. Our results are in agreement with other research studies [61,[88][89][90].…”

Section: Discussionsupporting

confidence: 94%

“…In order to assess the particle size and distribution, we prepared an aqueous suspension according to the method described in previous research published recently by our group [61]. The hydrodynamic diameter of the Fe 3 O 4 @C nanoparticles was determined at 25 • C, and from Figure 4 it can be observed that the nanoparticles were monomodal in nature.…”

Section: Dls Measurementsmentioning

confidence: 99%

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Development and Characterization of Fe3O4@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration

et al. 2021

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The current study presents the effect of naked Fe3O4@Carbon nanoparticles obtained by the combustion method on primary human gingival fibroblasts (HGFs) and primary gingival keratinocytes (PGKs)—relevant cell lines of buccal oral mucosa. In this regard, the objectives of this study were as follows: (i) development via combustion method and characterization of nanosized magnetite particles with carbon on their surface, (ii) biocompatibility assessment of the obtained magnetic nanoparticles on HGF and PGK cell lines and (iii) evaluation of possible irritative reaction of Fe3O4@Carbon nanoparticles on the highly vascularized chorioallantoic membrane of a chick embryo. Physicochemical properties of Fe3O4@Carbon nanoparticles were characterized in terms of phase composition, chemical structure, and polymorphic and molecular interactions of the chemical bonds within the nanomaterial, magnetic measurements, ultrastructure, morphology, and elemental composition. The X-ray diffraction analysis revealed the formation of magnetite as phase pure without any other secondary phases, and Raman spectroscopy exhibit that the pre-formed magnetic nanoparticles were covered with carbon film, resulting from the synthesis method employed. Scanning electron microscopy shown that nanoparticles obtained were uniformly distributed, with a nearly spherical shape with sizes at the nanometric level; iron, oxygen, and carbon were the only elements detected. While biological screening of Fe3O4@Carbon nanoparticles revealed no significant cytotoxic potential on the HGF and PGK cell lines, a slight sign of irritation was observed on a limited area on the chorioallantoic membrane of the chick embryo.

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Section: Discussionsupporting

confidence: 94%

Section: Dls Measurementsmentioning

confidence: 99%

Development and Characterization of Fe3O4@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration

et al. 2021

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“…The XRF analysis showed that the IONPs obtained by green synthesis could be used in biomedical applications because none of the samples obtained contains traces of metals which can be harmful in healthy living organism. This aspect is in agreement with other research studies, which affirm that nanoparticles based on iron oxides are biocompatible having a great attraction in biomedical treatments due to their non-toxicity [22][23][24][25][26][80][81][82].…”

Section: Xrf Analysissupporting

confidence: 93%

Biosynthesis of Iron Oxide Nanoparticles: Physico-Chemical Characterization and Their In Vitro Cytotoxicity on Healthy and Tumorigenic Cell Lines

et al. 2022

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Iron oxide nanoparticles were synthesized starting from two aqueous extracts based on Artemisia absinthium L. leaf and stems, employing a simplest, eco-friendliness and low toxicity method—green synthesis. The nanoparticles were characterized by powder X-ray diffraction (XRD), Fourier transformed infrared spectroscopy (FT-IR), X-ray fluorescence analysis (XRF), thermal analysis (TG/DSC), and scanning electron microscopy (SEM). Lack of magnetic properties and the reddish-brown color of all the samples confirms the presence of hematite as majority phase. The FTIR bands located at 435 cm−1 and 590 cm−1, are assigned to Fe-O stretching vibration from hematite, confirming the formation of α-Fe2O3 nanoparticles (NPs). The in vitro screening of the samples revealed that the healthy cell line (HaCaT) presents a good viability (above 80%) after exposure to iron oxide NPs and lack of apoptotic features, while the tumorigenic cell lines manifested a higher sensitivity, especially the melanoma cells (A375) when exposed to concentration of 500 µg/mL iron oxide NPs for 72 h. Moreover, A375 cells elicited significant apoptotic markers under these parameters (concentration of 500 µg/mL iron oxide NPs for a contact time of 72 h).

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“…G-M55 revealed good performances as a bimodal diagnostic contrast agent and as a therapeutic agent in MFH, with either standard or MPI-based systems. Our results demonstrate the efficacy of MFH in the treatment of breast cancer [ 8 , 22 , 23 , 24 ].…”

Section: Discussionsupporting

confidence: 57%

Doped Ferrite Nanoparticles Exhibiting Self-Regulating Temperature as Magnetic Fluid Hyperthermia Antitumoral Agents, with Diagnostic Capability in Magnetic Resonance Imaging and Magnetic Particle Imaging

Vurro

Gerosa

Busato

et al. 2022

Cancers

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This paper reports a comprehensive investigation of a magnetic nanoparticle (MNP), named M55, which belongs to a class of innovative doped ferrite nanomaterials, characterized by a self-limiting temperature. M55 is obtained from M48, an MNP previously described by our group, by implementing an additional purification step in the synthesis. M55, after citrate and glucose coating, is named G-M55. The present study aimed to demonstrate the properties of G-M55 as a diagnostic contrast agent for MRI and magnetic particle imaging (MPI), and as an antitumoral agent in magnetic fluid hyperthermia (MFH). Similar specific absorption rate values were obtained by standard MFH and by an MPI apparatus. This result is of interest in relation to the application of localized MFH by MPI apparatus. We demonstrated the biocompatibility of G-M55 in a triple-negative human breast cancer line (MDA-MB-231), and its efficacy as an MFH agent in the same cell line. We also demonstrated the efficacy of MFH treatment with G-M55 in an experimental model of breast cancer. Overall, our results pave the way for the clinical application of G-M55 as an MFH agent in breast cancer therapy, allowing not only efficient treatment by both standard MFH apparatus and MPI but also temperature monitoring.

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Biocompatible Magnetic Colloidal Suspension Used as a Tool for Localized Hyperthermia in Human Breast Adenocarcinoma Cells: Physicochemical Analysis and Complex In Vitro Biological Profile

Cited by 13 publications

References 76 publications

Development and Characterization of Fe3O4@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration

Development and Characterization of Fe3O4@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration

Biosynthesis of Iron Oxide Nanoparticles: Physico-Chemical Characterization and Their In Vitro Cytotoxicity on Healthy and Tumorigenic Cell Lines

Doped Ferrite Nanoparticles Exhibiting Self-Regulating Temperature as Magnetic Fluid Hyperthermia Antitumoral Agents, with Diagnostic Capability in Magnetic Resonance Imaging and Magnetic Particle Imaging

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