Biocompatible Infinite‐Coordination‐Polymer Nanoparticle–Nucleic‐Acid Conjugates for Antisense Gene Regulation

Abstract

Herein, we report the synthesis of DNA‐functionalized infinite‐coordination‐polymer (ICP) nanoparticles as biocompatible gene‐regulation agents. ICP nanoparticles were synthesized from ferric nitrate and a ditopic 3‐hydroxy‐4‐pyridinone (HOPO) ligand bearing a pendant azide. Addition of FeIII to a solution of the ligand produced nanoparticles, which were colloidally unstable in the presence of salts. Conjugation of DNA to the FeIII–HOPO ICP particles by copper‐free click chemistry afforded colloidally stable nucleic‐acid nanoconstructs. The DNA–ICP particles, when cross‐linked through sequence‐specific hybridization, exhibited narrow, highly cooperative melting transitions consistent with dense DNA surface loading. The ability of the DNA–ICP particles to enter cells and alter protein expression was also evaluated. Our results indicate that these novel particles carry nucleic acids into mammalian cells without the need for transfection agents and are capable of efficient gene knockdown.

Synthesis of Coordination Polymer Nanoparticles using Self‐Assembled Block Copolymers as Template

“…The coverage of the surface of the NPs leads to an increased stability and enhanced cellular uptake compared with “naked” MOF NPs. Further examples for DNA stabilized coordination network NPs from the same group showed that those particles enter cells and can alter protein expression, by this they are capable of an efficient gene knock‐down . Alternatively, the surface of nanoscale CPs can be functionalized with small interfering RNA for efficient drug delivery of cis ‐platinum to tumor cells .…”

Post modification of zinc based coordination polymer to prepare Zn‐Mo‐ICP nanoparticles as efficient self‐supported catalyst for olefin epoxidation

“…The resulting ICP particles have typically amorphous, rather than crystalline structures. These compounds have several applications in gas storage, mixture separations, antisense gene regulation, optoelectronics, and drug delivery . Many crystalline metal–organic framework catalysts are reported, but few utilization of nano/micro infinite coordination polymers as catalysts has been described …”

Molecular spherical nucleic acids

“…molecular nanoconjugates | spherical nucleic acids | oligonucleotides | gene regulation S pherical nucleic acids (SNAs) are polyvalent nanostructures made from particles chemically modified with a dense shell of highly oriented oligonucleotides (1). The first SNAs were made using gold nanoparticle (AuNP) cores (2), but the core compositions explored since then have included a wide variety of inorganic particles [silver (3), quantum dots (4), iron oxide (5), and silica (6)], organic materials [liposomes (7,8), polymers (9), proteins (10), and other macromolecules (11,12)], and hybrid structures [metal−organic frameworks (13) and infinite coordination polymers (14) in general]. In addition, core-less, hollow SNAs consisting of cross-linked oligonucleotides (15) have been developed, which exhibit many of the hallmark properties of the original AuNP structures.…”