Biocompatible Gels of Chitosan–Buriti Oil for Potential Wound Healing Applications

Ferreira, Maria Onaira Gonçalves; Lima, Idglan S. de; Ribeiro, Alessandra Braga; Lobo, Anderson Oliveira; Rizzo, Márcia dos Santos; Osajima, Josy A.; Estevinho, Letí­cia M.; Filho, Edson Cavalcanti da Silva

doi:10.3390/ma13081977

Cited by 26 publications

(28 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This controlled response promoted an improvement in wound tissue healing. These results corroborate the healing properties of other oils, such as pequi and buriti oil, which stimulate the healing of experimental skin wounds in rats [52,53].…”

Section: Plos Onesupporting

confidence: 83%

Rhynchophorus palmarum (Linnaeus, 1758) (Coleoptera: Curculionidae): Guarani-Kaiowá indigenous knowledge and pharmacological activities

et al. 2021

View full text Add to dashboard Cite

Zootherapy is a traditional secular practice among the Guarani-Kaiowá indigenous ethnic group living in Mato Grosso do Sul, Brazil. My people use the oil extracted from larvae of the snout beetle Rhynchophorus palmarum (Linnaeus, 1758) to treat and heal skin wounds and respiratory diseases. Based on this ethnopharmacological knowledge, the chemical composition and antioxidant, antimicrobial, and healing properties of R. palmarum larvae oil (RPLO) were investigated, as well as possible toxic effects, through in vitro and in vivo assays. The chemical composition of the RPLO was determined using gas chromatography coupled with mass spectrometry. The antioxidant activity of RPLO was investigated through the direct 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and the antimicrobial activity was evaluated against Gram-positive and Gram-negative bacteria that are pathogenic to humans. The healing properties of RPLO were investigated by performing a cell migration assay using human lung fibroblasts (MRC-5), and the toxicity was analyzed, in vivo, using a Caenorhabditis elegans model and MRC-5 cells, in vitro. RPLO contains 52.2% saturated fatty acids and 47.4% unsaturated fatty acids, with palmitic acid (42.7%) and oleic acid (40%) representing its major components, respectively. RPLO possesses direct antioxidant activity, with a half-maximal inhibitory concentration (IC50) of 46.15 mg.ml-1. The antimicrobial activity of RPLO was not observed at a concentration of 1% (v/v). RPLO did not alter the viability of MRC-5 cells and did not exert toxic effects on C. elegans. Furthermore, MRC-5 cells incubated with 0.5% RPLO showed a higher rate of cell migration than that of the control group, supporting its healing properties. Taken together, RPLO possesses direct antioxidant activity and the potential to aid in the healing process and is not toxic toward in vitro and in vivo models, corroborating the safe use of the oil in traditional Guarani-Kaiowá medicine.

show abstract

Section: Plos Onesupporting

confidence: 83%

Rhynchophorus palmarum (Linnaeus, 1758) (Coleoptera: Curculionidae): Guarani-Kaiowá indigenous knowledge and pharmacological activities

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The FTIR spectra of the prepared HCG and HCGP dry samples showed bands characteristic of the functional groups of CG and PAM polymers. The band observed in the range between 3600 and 3000 cm −1 may be attributed to the overlap of the N–H and O–H stretching vibration [ 56 , 57 ]; the overlapping of the bands may have occurred due to the presence of physically adsorbed water, since this presence greatly expands the band in this region, while the band at 1669 cm −1 corresponds to the C=O stretching present in the PAM structure. The decrease in the intensity of this band was noted for HCG and HCGP; this result is probably associated with the hydrolysis reactions, in which the amide groups (CONH 2 ) were converted to carboxylate groups (COONa) [ 58 ].…”

Section: Resultsmentioning

confidence: 99%

Superabsorbent Hydrogels Based to Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients

et al. 2021

Self Cite

View full text Add to dashboard Cite

Agricultural production is influenced by the water content in the soil and availability of fertilizers. Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed. The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS). Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test. The results showed that PHP incorporation in the hydrogel favored the crosslinking of chains. This increased the thermal stability in HCGP but decreased the hardness and adhesion properties. The HCGP demonstrated good swelling capacity (~15,000 times) and an excellent potential for reuse after fifty-five consecutive cycles. The swelling was favored in an alkaline pH due to the ionization of hydrophilic groups. The sustained release of phosphorus in HCGP was described by the Korsmeyer–Peppas model, and Fickian diffusion is the main fertilizer release mechanism. Finally, the hydrogels do not demonstrate toxicity, and HCGP has potential for application in agriculture.

show abstract

“…In this regard, printability, fidelity, viscoelasticity, expense, and cross-linking duration are some of the crucial factors relevant to the choice of bio-inks. The presence of the cells inside the ink to utilize a bio-ink provides the possibility to print 3D structures which are often implanted into impaired bone tissue to enhance cell responses [82,[154][155][156][157][158][159][160][161]. CS can be used as a bio ink either in pure form or in combination with other materials, especially other polymers [154,155].…”

Section: Bio-inksmentioning

confidence: 99%

“…Altering manufacturing parameters makes it possible for stimulating natural tissues with tailored biological characteristics appropriate for the recovery of tissues. The advantages of CS are actually broadly described; among the major positive aspects of CS, one can mention the capability to modify properties such as the degradation rate by altering the degree of deacetylation and it's Mw [157][158][159][160][161][162][163][164][165][166][167][168]. Additive components are regularly incorporated into CS in an effort to enhance its printability, fidelity, or to fabricate new cell-laden matrices.…”

Section: Benefits Limitations and Future Prospectsmentioning

confidence: 99%

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

et al. 2020

View full text Add to dashboard Cite

Chitosan (CS) has gained particular attention in biomedical applications due to its biocompatibility, antibacterial feature, and biodegradability. Hence, many studies have focused on the manufacturing of CS films, scaffolds, particulate, and inks via different production methods. Nowadays, with the possibility of the precise adjustment of porosity size and shape, fiber size, suitable interconnectivity of pores, and creation of patient-specific constructs, 3D printing has overcome the limitations of many traditional manufacturing methods. Therefore, the fabrication of 3D printed CS scaffolds can lead to promising advances in tissue engineering and regenerative medicine. A review of additive manufacturing types, CS-based printed constructs, their usages as biomaterials, advantages, and drawbacks can open doors to optimize CS-based constructions for biomedical applications. The latest technological issues and upcoming capabilities of 3D printing with CS-based biopolymers for different applications are also discussed. This review article will act as a roadmap aiming to investigate chitosan as a new feedstock concerning various 3D printing approaches which may be employed in biomedical fields. In fact, the combination of 3D printing and CS-based biopolymers is extremely appealing particularly with regard to certain clinical purposes. Complications of 3D printing coupled with the challenges associated with materials should be recognized to help make this method feasible for wider clinical requirements. This strategy is currently gaining substantial attention in terms of several industrial biomedical products. In this review, the key 3D printing approaches along with revealing historical background are initially presented, and ultimately, the applications of different 3D printing techniques for fabricating chitosan constructs will be discussed. The recognition of essential complications and technical problems related to numerous 3D printing techniques and CS-based biopolymer choices according to clinical requirements is crucial. A comprehensive investigation will be required to encounter those challenges and to completely understand the possibilities of 3D printing in the foreseeable future.

show abstract

Biocompatible Gels of Chitosan–Buriti Oil for Potential Wound Healing Applications

Cited by 26 publications

References 42 publications

Rhynchophorus palmarum (Linnaeus, 1758) (Coleoptera: Curculionidae): Guarani-Kaiowá indigenous knowledge and pharmacological activities

Rhynchophorus palmarum (Linnaeus, 1758) (Coleoptera: Curculionidae): Guarani-Kaiowá indigenous knowledge and pharmacological activities

Superabsorbent Hydrogels Based to Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

Contact Info

Product

Resources

About