Biocompatible Fluorescent Nanodiamonds as Multifunctional Optical Probes for Latent Fingerprint Detection

Abstract

There is an immense literature on detection of latent fingerprints (LFPs) with fluorescent nanomaterials because fluorescence is one of the most sensitive detection methods. Although many fluorescent probes have been developed for latent fingerprint detection, many challenges remain, including the low selectivity, complicated processing, high background, and toxicity of nanoparticles used to visualize LFPs. In this study, we demonstrate biocompatible, efficient, and low background LFP detection with poly­(vinylpyrrolidone) (PVP) coated fluorescent nanodiamonds (FNDs). PVP-coated FND (FND@PVP) is biocompatible at the cellular level. They neither inhibit cellar proliferation nor induce cell death via apoptosis or other cell killing pathways. Moreover, they do not elicit an immune response in cells. PVP coating enhances the physical adhesion of FND to diverse substrates and in particular results in efficient binding of FND@PVP to fingerprint ridges due to the intrinsic amphiphilicity of PVP. Clear, well-defined ridge structures with first, second, and third-level of LFP details are revealed within minutes by FND@PVP. The combination of this binding specificity and the remarkable optical properties of FND@PVP permits the detection of LPFs with high contrast, efficiency, selectivity, sensitivity, and reduced background interference. Our results demonstrate that background-free imaging via multicolor emission and dual-modal imaging of FND@PVP nanoparticles have great potential for high-resolution imaging of LFPs.

Nanodiamond-induced modifications of Eu-doped phosphate glasses toward photonic applications: A synergistic physico-chemical approach

“…A Gaussian absorption band with peak at 3.9 eV (318 nm) and full-width at half-maximum (FWHM) of 0.8 eV is revealed after the spectral subtraction. The absorption band peak position agrees with the lower of the two Eu 2+ crystal field split 4f 7 -4f 6 5d electronic level transitions, namely e g . Nogami et al 18 reported the two absorption bands at 3.9 eV (e g ) and 5.1 eV (t 2g ) in aluminosilicate glass prepared by sol-gel method.…”

“…E T g has slope of À8 eV, compared to E opt fit line with slope of 1.5 eV. 7 -4f 6 5d transition was deduced from the absorption spectra and utilized to explain the optical band gap narrowing concentration trend. The novel method for quantifying the photoluminescent spectra suggested was correlated to the C ND concentration and to the optical band gap.…”

“…nanodiamond (ND), have prompted investigations regarding their potential utilization for a wide variety of applications, ranging from biotechnology to integration in photonic devices. [1][2][3][4][5][6][7][8] The exceptional mechanical and optical properties coupled to the high surface areas intrinsic to nanoscale materials are some of the key characteristics facilitating various technologies. 2,5 On the other hand, ND also holds high chemical stability (low oxidative stress), exhibiting high resistance to harsh environments.…”

Carbon Dots as New Generation Materials for Nanothermometer: Review

“…Among the family of carbon-based nanomaterials, fluorescent nanodiamonds have been first reported as nanothermometers [ 53 ]. Fluorescent nanodiamonds possess intrinsic biocompatibility resulting from their chemically robust and inert surface [ 54 ]. Other nanodiamonds have recently been used for intracellular thermal sensing with sub-degree accuracy [ 55 , 56 ].…”

Nanodiamond-containing composites for tissue scaffolds and surgical implants: A review

“…Moreover, nanodi- amonds are highly biocompatible, they can be easily functionalized like bioconjugation, and they have a small particle size of about 5 nm. A few products based on carbon nanomaterial such as derivatized fullerenes [5][6][7][8] or adamantane derivatives (e.g. memantine, amantadine, and rimantadine) have found ways to medicinal practice; however, ND suspensions have offered promising outcomes when used in animals and human patients suffering from cancer [9][10][11][12].…”