Biocompatible Electroactive Tetra(aniline)-Conjugated Peptide Nanofibers for Neural Differentiation

Arioz, Idil; Erol, Özlem; Bakan, Gökhan; Dikecoglu, F. Begum; Topal, Ahmet E.; Ürel, Mustafa; Dâna, Aykutlu; Tekinay, Ayşe B.; Güler, Mustafa O.

doi:10.1021/acsami.7b16509

Cited by 45 publications

(41 citation statements)

References 46 publications

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“…The natural ECM encompasses a 3D network of intertwined protein nanofibers which contains complex biomolecules for communication between cells . Short peptide‐based 3D nanostructured supramolecular hydrogels are excellent candidates for ECM mimicry as they provide networks of fibers which resemble the ECM structure . Modifications of certain oligopeptide termini with bio‐active ligands, aimed to introduce bio‐active components into the system, have also been reported .…”

Section: Figurementioning

confidence: 99%

Composite of Peptide‐Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio‐Inspired Soft Material

Chakraborty

Ghosh

Schnaider

et al. 2019

Macromol. Rapid Commun.

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Engineering of extracellular matrix (ECM) mimics, with most of the necessary features of a natural ECM, is a crucial requirement for the design of biomaterials. The natural ECM encompasses a 3D network of intertwined protein nanofibers which contains complex biomolecules for communication between cells.[1] Short peptide-based 3D nanostructured supramolecular hydrogels are excellent candidates for ECM mimicry as they provide networks of fibers which resemble the ECM structure. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] Modifications of certain oligopeptide termini with bio-active ligands, aimed to introduce bio-active components into the system, have also been reported. [22][23][24][25] The tripeptide sequence Arg-Gly-Asp (RGD), which was first recognized in fibronectin as an independent cell attachment site, is the most commonly used bio-active ligand. [26] This tri-peptide sequence is recognized by the α v β 3 and α 5 β 1 integrins located in cell membranes, thus facilitating the coupling of the ECM with the cytoskeleton. [27] Owing to these attributes, the RGD ligand has been introduced into supramolecular or polymeric substrates to facilitate cell attachment. [28][29][30][31] Fmoc-RGD [Fmoc = N-(fluorenyl-9-methoxycarbonyl)] is one of the simplest RGD derivatives capable of forming hydrogels with an inter-connected fibrous network. [15,27] Ulijn's group reported Fmoc-RGD based supramolecular hydrogels in neutral aqueous solution by appropriate mixing of two short peptidebased building blocks, Fmoc-FF (F = phenylalanine) and Fmoc-RGD and utilized them as scaffolds for cell culture. [15] They proposed that Fmoc-RGD alone was not able to form β-sheet fibrils, although in the composite gels of Fmoc-FF and Fmoc-RGD (10-30 wt% Fmoc-RGD), signatures of β-sheet structures were found. We later reported flanking of the RGD sequence by aromatic moieties, demonstrating the hydrogelation of RGD derivatives, including Fmoc-FRGD and Fmoc-RGDF. [32] Hamley and co-workers showed that the simple Fmoc-RGD moiety, without any modification or mixing with other hydrogelator peptides, could from nano-fibrous hydrogels. [27] Castelletto and co-workers prepared monoliths of Fmoc-RGD gels at a high peptide concentration of 10 wt% which showed excellent Bio-Inspired Soft MaterialsPeptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery. The Arg-Gly-Asp (RGD) ligand based supramolecular gels have immense potential in this respect, as this tripeptide is known to promote cell adhesion. Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture. In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrog...

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Section: Figurementioning

confidence: 99%

Composite of Peptide‐Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio‐Inspired Soft Material

Chakraborty

Ghosh

Schnaider

et al. 2019

Macromol. Rapid Commun.

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“…Nanofibrous polymeric scaffolds have been extensively researched to mimic the natural extracellular matrix (ECM). The CP nanofibers can be synthesized by electrospinning, phase separation, and molecular assembly [108][109][110]. In the process of electrospinning, the CPs are mixed with biodegradable polymers, then electrospun into the form of nanofibers.…”

Section: Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Research Progress on Conducting Polymer-Based Biomedical Applications

2019

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Conducting polymers (CPs) have attracted significant attention in a variety of research fields, particularly in biomedical engineering, because of the ease in controlling their morphology, their high chemical and environmental stability, and their biocompatibility, as well as their unique optical and electrical properties. In particular, the electrical properties of CPs can be simply tuned over the full range from insulator to metal via a doping process, such as chemical, electrochemical, charge injection, and photo-doping. Over the past few decades, remarkable progress has been made in biomedical research including biosensors, tissue engineering, artificial muscles, and drug delivery, as CPs have been utilized as a key component in these fields. In this article, we review CPs from the perspective of biomedical engineering. Specifically, representative biomedical applications of CPs are briefly summarized: biosensors, tissue engineering, artificial muscles, and drug delivery. The motivation for use of and the main function of CPs in these fields above are discussed. Finally, we highlight the technical and scientific challenges regarding electrical conductivity, biodegradability, hydrophilicity, and the loading capacity of biomolecules that are faced by CPs for future work. This is followed by several strategies to overcome these drawbacks.

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“…Many biological processes involve redox reactions and thus EAAs have already been investigated for use in a wide range of research areas. For example, EAAs that self‐assemble into fibrillar network structures have been used as scaffolds to facilitate nerve regeneration and promote more advanced neural differentiation than nonconductive materials . In a different approach, ferrocene‐containing EAAs have been used to mimic cationic lipids that can transfer DNA across cell membranes, promoting gene expression.…”

Section: Switching Functionmentioning

confidence: 99%

“…For example, EAAs that self-assemble into fibrillar network structures have been used as scaffolds to 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 facilitate nerve regeneration and promote more advanced neural differentiation than nonconductive materials. [71] In a different approach, ferrocene-containing EAAs have been used to mimic cationic lipids that can transfer DNA across cell membranes, promoting gene expression. In the reduced state, the EAA promotes high cell transfection, while the oxidised state shows very low levels.…”

Section: Biological Processesmentioning

confidence: 99%

Electroactive Amphiphiles for Addressable Supramolecular Nanostructures

et al. 2018

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In this focus review we aim to highlight an exciting class of materials, electroactive amphiphiles (EAAs). This class of functional amphiphilic molecules has been the subject of sporadic investigations over the last few decades, but little attempt has been made to date to gather or organise these investigations into a logical fashion. Here we attempted to gather the most important contributions, provide a framework in which to discuss them, and, more importantly, point towards the areas where we believe these EAAs will contribute to solving wider scientific problems and open new opportunities. Our discussions cover materials based on low molecular weight ferrocenes, viologens and anilines, as well as examples of polymeric and supramolecular EAAs. With the advances of modern analytical techniques and new tools for modelling and understanding optoelectronic properties, we believe that this area of research is ready for further exploration and exploitation.

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Biocompatible Electroactive Tetra(aniline)-Conjugated Peptide Nanofibers for Neural Differentiation

Cited by 45 publications

References 46 publications

Composite of Peptide‐Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio‐Inspired Soft Material

Composite of Peptide‐Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio‐Inspired Soft Material

Research Progress on Conducting Polymer-Based Biomedical Applications

Electroactive Amphiphiles for Addressable Supramolecular Nanostructures

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