Biocompatible capped iron oxide nanoparticles for Vibrio cholerae detection

Abstract

We report the studies relating to fabrication of an efficient immunosensor for Vibrio cholerae detection. Magnetite (iron oxide (Fe(3)O(4))) nanoparticles (NPs) have been synthesized by the co-precipitation method and capped by citric acid (CA). These NPs were electrophoretically deposited onto indium-tin-oxide (ITO)-coated glass substrate and used for immobilization of monoclonal antibodies against Vibrio cholerae (Ab) and bovine serum albumin (BSA) for Vibrio cholerae detection using an electrochemical technique. The structural and morphological studies of Fe(3)O(4) and CA-Fe(3)O(4)/ITO were characterized by x-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) techniques. The average crystalline size of Fe(3)O(4), CA-Fe(3)O(4) nanoparticles obtained were about 29 ± 1 nm and 37 ± 1 nm, respectively. The hydrodynamic radius of the nanoparticles was found to be 77.35 nm (Fe(3)O(4)) and 189.51 nm (CA-Fe(3)O(4)) by DLS measurement. The results of electrochemical response studies of the fabricated BSA/Ab/CA-Fe(2)O(3)/ITO immunosensor exhibits a good detection range of 12.5-500 ng mL(-1) with a low detection limit of 0.32 ng mL(-1), sensitivity 0.03 Ω/ng ml(-1) cm(-2), and reproducibility more than 11 times.

Synthesis of Chitosan-Mediated Silver Coated γ-Fe2O3 (Ag−γ-Fe2O3@Cs) Superparamagnetic Binary Nanohybrids for Multifunctional Applications

“…FESEM images of IO, CIO, CIOLAg, and CIOMAg are shown in Figure and their respective EDX analyses are shown in Figure S3a, Supporting Information. The FESEM image of IO exhibits the formation of clusters possibly arising due to the tendency of aggregation of the magnetic NPs . The capping of IO by chitosan in CIO nanohybrids results in the reduction in the size of these clusters (Figure b).…”

“…The FESEM image of IO exhibits the formation of clusters possibly arising due to the tendency of aggregation of the magnetic NPs. 5 The capping of IO by chitosan in CIO nanohybrids results in the reduction in the size of these clusters (Figure 5b). It possibly occurs due to the supramolecular interactions involving the functionalities of chitosan and IO NPs, and thereby enhancing the repulsion among the IO NPs.…”

“…1,2 These properties render them to be of great interest for a wide range of applications such as magnetic fluids, magnetic energy storage, information storage, spintronics, catalysis, 3 and biomedical. 4,5 In most of the envisaged biomedical applications, these particles have been found to serve the best purpose in a critical size ranging from 5 to 20 nm and are also dependent on their structure. 6 Among various developed magnetic nanosystems, magnetite (Fe 3 O 4 ) and maghemite (γ-Fe 2 O 3 ) are the only FDA approved magnetic NPs as a support for biological applications 7 and have been widely investigated because of their nontoxicity, biocompatibility, and high magnetization.…”

“…In recent years, a deep interest has arisen in the study of magnetic nanoparticles (NPs) because of their tunable fundamental properties such as optical, electronic, magnetic, catalytic, and biological besides being economically viable. , These properties render them to be of great interest for a wide range of applications such as magnetic fluids, magnetic energy storage, information storage, spintronics, catalysis, and biomedical. , In most of the envisaged biomedical applications, these particles have been found to serve the best purpose in a critical size ranging from 5 to 20 nm and are also dependent on their structure …”

Transferrin-Decorated Niosomes with Integrated InP/ZnS Quantum Dots and Magnetic Iron Oxide Nanoparticles: Dual Targeting and Imaging of Glioma

“…Citrate-coated magnetic iron oxide nanoparticles (CA-MIONs) were synthesized via a co-precipitation method [ 41 , 52 ]. 0.75 g of FeCl 3 ·6H 2 O and 0.375 g of FeCl 2 ·4H 2 O were dissolved in 50 mL of distilled water and mixed at 80 °C under nitrogen atmosphere in a 100 mL three-neck flask equipped with a reflux condenser.…”