Biocompatible and antimicrobial multilayer fibrous polymeric wound dressing with optimally embedded silver nanoparticles

Sarviya, Nandini; Mahanta, Urbashi; Dart, Alexander; Giri, Jyotsnendu; Deshpande, Atul Suresh; Khandelwal, Mudrika; Bhave, Mrinal; Kingshott, Peter

doi:10.1016/j.apsusc.2022.155799

Cited by 14 publications

(18 citation statements)

References 49 publications

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“…In the case of cotton fabric coated with WK-PVA nanofibers, cell growth at day 1 was 92.7%, which gradually increased to 100.5% over 5 days. However, the cotton fabric coated with 1AgNP-WK-PVA nanofibers showed a decrease in cell growth over 5 days, which is consistent with published results . On day 1, the L-929 fibroblast cell growth was 92.5%, which decreased to 81.6% after 5 days of incubation.…”

Section: Resultssupporting

confidence: 91%

“…However, the cotton fabric coated with 1AgNP-WK-PVA nanofibers showed a decrease in cell growth over 5 days, which is consistent with published results. 34 On day 1, the L-929 fibroblast cell growth was 92.5%, which decreased to 81.6% after 5 days of incubation. The cell growth considerably decreased when the Ag content of the WK-PVA nanofibrous membrane deposited on the cotton fabric was increased to 2% (i.e., 2AgNP-WK-PVA).…”

Section: Cell Viability By Mtt Bioassay and Cell Proliferationmentioning

confidence: 91%

“…21 Electrospun nanofibrous wound dressing of keratin extracted from wool fibers, hair, and feathers has been studied for wound healing. Several articles reported the Ag ion release characteristics of Ag NP-containing nanofibrous membranes, 34,35 but no article reported the release characteristics of Ag ions from Ag NPcontaining keratin/PVA composite nanofibrous membrane deposited on cotton fabrics. Wool keratin has an advantage over other natural polymers as it is a natural protein polymer with excellent cytocompatibility.…”

Section: Introductionmentioning

confidence: 99%

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Wound Dressing Based on Silver Nanoparticle Embedded Wool Keratin Electrospun Nanofibers Deposited on Cotton Fabric: Preparation, Characterization, Antimicrobial Activity, and Cytocompatibility

Hassan,

Heins,

Zheng

2024

ACS Appl. Bio Mater.

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Wool keratin (WK) protein is attractive for wound dressing and biomedical applications due to its excellent biodegradability, cytocompatibility, and wound-healing properties. In this work, WK-based wound dressings were prepared by depositing WK/poly(vinyl alcohol) (PVA) and silver nanoparticle (Ag NP)-embedded WK/PVA composite nanofibrous membranes on cotton fabrics by electrospinning. Ag NPs were biosynthesized by reduction and stabilization with sodium alginate. The formed Ag NPs were characterized by ultraviolet–visible and Fourier transform infrared (FTIR) spectroscopy, and their size was determined by transmission electron microscopy and image analysis. The formed Ag NPs were spherical and had an average diameter of 9.95 nm. The produced Ag NP-embedded WK/PVA composite nanofiber-deposited cotton fabric surface was characterized by FTIR and dynamic contact angle measurements, and the nanofiber morphologies were characterized by scanning electron microscopy. The average diameter of the nanofibers formed by 0.1% Ag NP-embedded WK/PVA solution was 146.7 nm. The antibacterial activity of the surface of cotton fabrics coated with electrospun composite nanofibers was evaluated against the two most common wound-causing pathogens, Staphylococcus aureus and Pseudomonas aeruginosa. The cotton fabric coated with 0.1% Ag NP-embedded WK/PVA nanofibers showed very good antibacterial activity against both pathogens, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay results showed good cytocompatibility against L-929 mouse fibroblast cells. However, the increase in Ag NP content in the nanofibers to 0.2% negatively affected the cell viability due to the high release rate of Ag ions. The results achieved show that the developed wound dressing has good potential for wound healing applications.

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Section: Resultssupporting

confidence: 91%

Section: Cell Viability By Mtt Bioassay and Cell Proliferationmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Wound Dressing Based on Silver Nanoparticle Embedded Wool Keratin Electrospun Nanofibers Deposited on Cotton Fabric: Preparation, Characterization, Antimicrobial Activity, and Cytocompatibility

Hassan,

Heins,

Zheng

2024

ACS Appl. Bio Mater.

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“…Skin abrasions and cuts are everyday experiences in one’s lifetime, often resolving without complications. However, complications such as infection can form chronic, non-healing, and hard-to-heal wounds 1 . Traditional signs of inflammation, such as pain, heat, and redness, emerge as the body fights off infection.…”

Section: Introductionmentioning

confidence: 99%

Silver-loaded poly(vinyl alcohol)/polycaprolactone polymer scaffold as a biocompatible antibacterial system

Vilamová,

Šimonová,

Bednář

et al. 2024

Sci Rep

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A chronic nonhealing wound poses a significant risk for infection and subsequent health complications, potentially endangering the patient‘s well-being. Therefore, effective wound dressings must meet several crucial criteria, including: (1) eliminating bacterial pathogen growth within the wound, (2) forming a barrier against airborne microbes, (3) promoting cell proliferation, (4) facilitating tissue repair. In this study, we synthesized 8 ± 3 nm Ag NP with maleic acid and incorporated them into an electrospun polycaprolactone (PCL) matrix with 1.6 and 3.4 µm fiber sizes. The Ag NPs were anchored to the matrix via electrospraying water-soluble poly(vinyl) alcohol (PVA), reducing the average sphere size from 750 to 610 nm in the presence of Ag NPs. Increasing the electrospraying time of Ag NP-treated PVA spheres demonstrated a more pronounced antibacterial effect. The resultant silver-based material exhibited 100% inhibition of gram-negative Escherichia coli and gram-positive Staphylococcus aureus growth within 6 h while showing non-cytotoxic effects on the Vero cell line. We mainly discuss the preparation method aspects of the membrane, its antibacterial properties, and cytotoxicity, suggesting that combining these processes holds promise for various medical applications.

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“…21 Therefore, Ag NPs have antibacterial properties against a wide range of pathogens, especially against E. coli (Gram-negative) and Staphylococcus aureus (Gram-positive)with more than 90%. 22,23 However, there are relatively few studies on the inhibition of fungus growth and reproduction by Ag NPs. Penicillium, a species of fungus commonly found in food, shoes, and clothing, is widely present in nature.…”

Section: Introductionmentioning

confidence: 99%

Preparation and property of antibacterial filter membrane by coaxial electro‐spraying/electrospinning technology

Ren,

Liu,

et al. 2023

J of Applied Polymer Sci

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Herein, a novel technology named coaxial electro‐spraying/electrospinning was successfully introduced, which can simultaneously prepare nanofibers and nanoparticles. First, the device contains an outer nozzle and an inner nozzle, which combine electrospinning with electro‐spraying technology. Then, the polyamide 6 (PA6) was spun to prepare the nanofibers from the inner nozzle, and the silver nanoparticles (Ag NPs) into the PA6 solution were sprayed to prepare the nanoparticles from the outer nozzle. PA6 nanofibers (NFs)/Ag nanoparticles (NPs) antibacterial composite membranes for the filtration of fine particulate pollutants were successfully prepared using coaxial electro‐spraying/electrospinning. The microstructure, physical properties, and antibacterial properties of the composite membrane were investigated. The results show that Ag NPs are successfully loaded in situ onto PA6 NFs of approximately 100 nm to form a three‐dimensional (3D) structure. The filtration efficiency of the 3D structured filtration membrane reaches 90.98%, compared to only 12.78% for the melt‐blown cloth mask filter material when tested with polystyrene microspheres (PS) of 200 nm. The contact angle of the composite membrane decreased from 145.47° to 44.11°, indicating a significant improvement in the hydrophilicity of the membrane. Furthermore, depositing Ag on the surface fibers resulted in an impressive antibacterial rate of 99.75% against Penicillium.

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Biocompatible and antimicrobial multilayer fibrous polymeric wound dressing with optimally embedded silver nanoparticles

Cited by 14 publications

References 49 publications

Wound Dressing Based on Silver Nanoparticle Embedded Wool Keratin Electrospun Nanofibers Deposited on Cotton Fabric: Preparation, Characterization, Antimicrobial Activity, and Cytocompatibility

Wound Dressing Based on Silver Nanoparticle Embedded Wool Keratin Electrospun Nanofibers Deposited on Cotton Fabric: Preparation, Characterization, Antimicrobial Activity, and Cytocompatibility

Silver-loaded poly(vinyl alcohol)/polycaprolactone polymer scaffold as a biocompatible antibacterial system

Preparation and property of antibacterial filter membrane by coaxial electro‐spraying/electrospinning technology

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