Biocompatibility of Polysulfone II. Platelet Adhesion and CHO Cell Growth

Khang, Gilson; Jeong, Bong Jin; Lee, Hai Bang; Park, Joon B.

doi:10.3233/bme-1995-5406

Cited by 5 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surface properties of the biodegradable polymer used in tissue engineering on the interaction of cells are one of the most important parameters when biomaterials of implant devices for tissue engineering are designed. It is recognized that the behavior of the adhesion and proliferation of different types of cells on polymer materials depend on the surface characteristics such as wettability, chemistry, charge, roughness, and rigidity 15, 19–33…”

Section: Introductionmentioning

confidence: 99%

Response of human chondrocytes on polymer surfaces with different micropore sizes for tissue‐engineered cartilage

Lee

Han³

et al. 2004

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

Response of chondrocytes on polymer surfaces is important for applications of tissue-engineered cartilage, and tissue engineering contains the interaction of cells on material surfaces. We examined the behavior of human chondrocytes cultured on polycarbonate (PC) membranes with different micropore sizes (0.2-8.0 m in diameter). The adhesion and proliferation of chondrocytes were evaluated by measuring the number of attached cells after 1, 2, and 4 days of culture and morphological observations. It seems that the cell adhesion and proliferation were progressively inhibited, as the PC membranes had micropores with increasing size, probably due to surface discontinuities produced by track-etched pores. On the PC membrane with smaller micropore sizes, the cells seemed to override these surface discontinuities. Phenotype of chondrocyte was as-sessed by Safranin-O staining for anionic sulfated proteoglycans. Chondrocytes on the 8.0 m-diameter membrane surface proliferated, on 8 days, formed morphology of round shape, and expressed proteoglycans, because of limitation of spreading by the track-etched micropores. They maintained their phenotype under conditions that support a round cell shape on the large pore diameter substrate, whereas chondrocyte phenotypes lost on the small pore diameter membranes. In conclusion, this study demonstrated that micropore sizes as well as pore-to-pore distance play an important role for adhesion, proliferation, and phenotype of human articular chondrocytes.

show abstract

Section: Introductionmentioning

confidence: 99%

Response of human chondrocytes on polymer surfaces with different micropore sizes for tissue‐engineered cartilage

Lee

Han³

et al. 2004

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

show abstract

“…It might be a suitable biomaterial for use in tissue engineered repair systems6–11 in which cells are cultured within PLGA films or scaffolds and in drug delivery systems12–16 in which drugs are loaded within PLGA microspheres. However, it is more desirable to change the hydrophobic PLGA surface to be hydrophilic for biomedical applications 17–19. Hydrophobic surfaces possess high interfacial free energy in aqueous solutions that tends to unfavorably influence their cell, tissue, and blood compatibility during the initial stage of contact 20…”

Section: Introductionmentioning

confidence: 99%

Interaction of different types of cells on physicochemically treated poly(L‐lactide‐co‐glycolide) surfaces

Khang

Choee

Rhee

et al. 2002

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

ABSTRACT:To improve the cell compatibility of poly(L-lactide-co-glycolide) (PLGA; 75/25 molar ratio of lactide to glycolide) surfaces, we experimented with physicochemical treatments. Chemical treatments employed 70% chloric acid, 50% sulfuric acid, and 0.5N sodium hydroxide solutions, and physical methods included corona and plasma treatments. The water contact angle of surface-treated PLGA decreased from 73 to 50 -60°; that is, the hydrophilicity increased because of the introduction of oxygencontaining functional groups onto the PLGA backbone according to electron spectroscopy for chemical analysis. The physicochemically modified PLGA surfaces were used to investigate the interaction of four different types of cells-hepatoma (Hep G2), osteoblast (MG 63), bovine aortic endothelial (CPAE), and fibroblast (NIH/3T3) cells-in terms of the surface hydrophilicity and hydrophobicity of PLGA. The cells that adhered and grew on the physicochemically modified PLGA surfaces were counted and observed with scanning electron microscopy. The adhesion and growth of Hep G2, MG 63, CPAE, and NIH/3T3 cells on physicochemically treated PLGA surfaces, especially on chloric acid-treated PLGA surfaces, were more active than on the control. This result seems closely related to the serum protein adsorption on the surface; the serum proteins were also adsorbed more on the hydrophilic surface. Surface hydrophilicity apparently plays an important role in cell adhesion, spreading, and growth on PLGA surfaces. The surface modification technique used in this study may be applicable to tissue engineering for the improvement of tissue compatibility of film-and scaffold-type substrates.

show abstract

Preparation and characterization of small intestine submucosa powder impregnated poly(L-lactide) scaffolds: the application for tissue engineered bone and cartilage

Khang

Rhee

Shin³

et al. 2002

Macromol. Res.

Self Cite

View full text Add to dashboard Cite

In order to endow with new bioactive functionality from small intestine submucosa (SIS) powder as natural source to poly(L-lactide) (PLA) and poly(lactide-co-glycolide) (PLGA) synthetic biodegradable polymer, porous SIS/PLA and SIS/PLGA as natural/synthetic composite scaffolds were prepared by means of the solvent casting/salt leaching methods for the possibility of the application of tissue engineered bone and cartilage. A uniform distribution of good interconnected pores from the surface to core region was observed the pore size of 40~500 µm independent with SIS amount using the solvent casting/salt leaching method. Porosities, specific pore areas as well as pore size distribution also were almost same. After the fabrication of SIS/PLA hybrid scaffolds, the wetting properties was greatly enhanced resulting in more uniform cell seeding and distribution. Five groups as PGA nonwoven mesh without glutaraldehyde (GA) treatment, PLA scaffold without or with GA treatment, and SIS/PLA (Code No. 3 ; 1 : 12 of salt content, 0.4 : 1 of SIS content, and 144 µm of median pore size) without or with GA treatment were implanted into the back of nude mouse to observe the effect of SIS on the induction of cells proliferation by hematoxylin and eosin, and von Kossa staining for 8 weeks. It was observed that the effect of SIS/PLA scaffolds with GA treatment on bone induction are stronger than PLA scaffolds, that is to say, in the order of PLA/SIS scaffolds with GA treatment *PLA/SIS scaffolds without GA treatment *PGA nonwoven *PLA scaffolds only with GA treatment = PLA scaffolds only without GA treatment for the osteoinduction activity. The possible explanations are (1) many kinds of secreted, circulating, and extracellular matrix-bound growth factors from SIS to significantly affect critical processes of tissue development and differentiation, (2) the exposure of SIS to GA resulted in significantly calcification, and (3) peri-implant fibrosis due to covalent bonding between collagen molecule by crosslinking reaction. In conclusion, it seems that SIS plays an important role for bone induction in SIS/PLA scaffolds for the application of tissue engineering area.

show abstract

Biocompatibility of Polysulfone II. Platelet Adhesion and CHO Cell Growth

Cited by 5 publications

References 0 publications

Response of human chondrocytes on polymer surfaces with different micropore sizes for tissue‐engineered cartilage

Response of human chondrocytes on polymer surfaces with different micropore sizes for tissue‐engineered cartilage

Interaction of different types of cells on physicochemically treated poly(L‐lactide‐co‐glycolide) surfaces

Preparation and characterization of small intestine submucosa powder impregnated poly(L-lactide) scaffolds: the application for tissue engineered bone and cartilage

Contact Info

Product

Resources

About