Interaction of different types of cells on physicochemically treated poly(L‐lactide‐co‐glycolide) surfaces

Abstract

ABSTRACT:To improve the cell compatibility of poly(L-lactide-co-glycolide) (PLGA; 75/25 molar ratio of lactide to glycolide) surfaces, we experimented with physicochemical treatments. Chemical treatments employed 70% chloric acid, 50% sulfuric acid, and 0.5N sodium hydroxide solutions, and physical methods included corona and plasma treatments. The water contact angle of surface-treated PLGA decreased from 73 to 50 -60°; that is, the hydrophilicity increased because of the introduction of oxygencontaining functional groups onto the PLGA backbone according to electron spectroscopy for chemical analysis. The physicochemically modified PLGA surfaces were used to investigate the interaction of four different types of cells-hepatoma (Hep G2), osteoblast (MG 63), bovine aortic endothelial (CPAE), and fibroblast (NIH/3T3) cells-in terms of the surface hydrophilicity and hydrophobicity of PLGA. The cells that adhered and grew on the physicochemically modified PLGA surfaces were counted and observed with scanning electron microscopy. The adhesion and growth of Hep G2, MG 63, CPAE, and NIH/3T3 cells on physicochemically treated PLGA surfaces, especially on chloric acid-treated PLGA surfaces, were more active than on the control. This result seems closely related to the serum protein adsorption on the surface; the serum proteins were also adsorbed more on the hydrophilic surface. Surface hydrophilicity apparently plays an important role in cell adhesion, spreading, and growth on PLGA surfaces. The surface modification technique used in this study may be applicable to tissue engineering for the improvement of tissue compatibility of film-and scaffold-type substrates.

The surface characterization and bioactivity of NANOZR in vitro

“…Many in vitro studies have investigated the relationship between the hydrophilicity of a material surface and cell adhesion. High surface wettability, which means a low contact angle, is generally reported to promote greater cell adhesion than a high contact angle [29][30][31][32] . The surface contact angles of the three tested substrates were not significantly different, which partly explains why the cell adhesion and proliferation dynamic of the three substrates were similar.…”

Biodegradable electrospun PLLA/chitosan membrane as guided tissue regeneration membrane for treating periodontitis

“…However, poor hydrophilicity limit the utility of PLLA [25]. Chitosan grafting is a common way to improve the properties of material such as bacteriostatic effect [26], enhancing adsorption properties [27], and improving biocompatibility [28].…”

Non-Thermal Plasma Surface Modification of Biodegradable Polymers

“…However, quite a few research articles deal with plasma modification of the copolymer PLGA (Khorasani et al, 2008, Hasirci et al, 2010, Khang et al, 2002, Park et al, 2007, Park et al, 2010, Safinia et al, 2007, Safinia et al, 2008, Shen et al, 2008, Wang et al, 2004. 50/50 PLGA films were modified in an oxygen plasma at low pressure and a decrease of the contact angle from 67° to below 40° after plasma treatment was observed.…”

“…Figure 4 shows that oxygen plasma treatment clearly improves attachment and growth of B65 cells, however, the effect of oxygen plasma treatment seems less pronounced as was the case for PLLA surfaces (see Figure 2). Khang et al studied and compared several modification methods including chemical methods (sulphuric acid, chloric acid, sodium hydroxide) as well as physical methods (atmospheric pressure air discharge) for the surface treatment of PLGA (Khang et al, 2002). Their results clearly evidenced that both chemical methods and plasma treatment could enhance cell attachment and growth.…”