Biocompatibility of Polyester Scaffolds with Fibroblasts and Osteoblast-like Cells for Bone Tissue Engineering

Abstract

Global gene expression profile of osteoblast-like cells grown on polyester co-polymer scaffolds. Manuscript.

Biocompatibility of various hydoxyapatite scaffolds evaluated by proliferation of rat’s bone marrow mesenchymal stem cells: an <em>in vitro</em> study

“…L929 is a cell line commonly used in cytotoxicity testing and HOB is a relevant cell line for application in bone regeneration. 8 Our study which evaluated the cytotoxicity of the various HA scaffolds used rat MSCs, http://mji.ui.ac.id…”

“…should be tested before it is implanted as scaffold, alone or in combination with demineralized bone matrix and or stem cells, in animal tests or clinical trials, 8 because it may reduce the probability of undesired effects such as; cell death, serious cell functional impairment, 8,14 and disturbance of healing process (fusion/union), 7 when used in stem cell transplantation. Moreover, in vitro studies may show a direct correlation between Table 1.…”

“…3,8 Those tests may be performed by direct and indirect contact toxicity. In direct contact toxicity test, we put granules of various scaffolds within plates and incubated together with MSCs.…”

“…It may also determine correlation between toxic effect of scaffold and cell death. [8][9][10] Indirect toxicity test was performed using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. This assay measure cellular metabolic activity via NAD(P)H-dependent cellular oxidoreductase enzymes and may, under defined conditions, reflect the number of viable cells (cell proliferation).…”

In Vivo Host Response and Degradation of Copolymer Scaffolds Functionalized with Nanodiamonds and Bone Morphogenetic Protein 2

“…[ 2 ] Synthetic polyesters have been used in the biomedical fi eld and for producing scaffolds for BTE for decades. [ 3 ] Degradable copolymers such as poly[( L -lactide)-co-(ε-caprolactone)] (PLCL) produced by copolymerization have been recently exploited for BTE scaffolds due to their in vitro cytocompatibility, [ 4 ] osteogenic conductivity [ 5 ] in addition to tuneable mechanical and degradable properties. [ 6 ] Also, scaffolds produced by blending of poly( L -lactide) and polycaprolactone in different proportions signifi cantly improved human stem cell attachment and growth when compared to homopolymers.…”