A novel rapid method for bacterial differentiation is explored based on the specific adhesion pattern of bacterial strains to tunable polymer surfaces. Different types of counter ions were used to electrochemically fabricate dissimilar polypyrrole (PPy) films with diverse physicochemical properties such as hydrophobicity, thickness and roughness. These were then modulated into three different oxidation states in each case. The dissimilar sets of conducting polymers were Principal Component Analysis showed that each strain of bacteria had its own specific adhesion pattern. Hence, they could be discriminated by this simple, label-free method. , based on tunable polymer arrays combined with pattern recognition.