Biocompatibility and stability of disulfide-crosslinked hyaluronan films

Liang, Yanchun; Shu, Xiao Zheng; Prestwich, Glenn D.

doi:10.1016/j.biomaterials.2005.01.003

Cited by 117 publications

(86 citation statements)

References 36 publications

(47 reference statements)

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“…After 20 days, 65% weight remained in the 100% HA but only 1% weight remained in the 100% FB. The degradation for combination hydrogels were as follows: 1, 1, 3, 11, 25, 33, 42, 50, and 50% of the weight remained for 10,20,30,40,50,60,70,80, and 90% HA, respectively. Degradation study did not include cells, and therefore no enzymatic degradation occurred.…”

Section: Resultsmentioning

confidence: 99%

“…Synthetic or semi-synthetic HA is usually subjected to many different modifications to improve its biomechanical as well as chemical properties. 20 Depending on the degree of chemical modification of HA, the resulting product can be either monolithic or living. 21 Living HA derivatives are more desirable in tissue engineering because they can form new covalent bonds in the presence of cells, tissue, or large molecules.…”

Section: Introductionmentioning

confidence: 99%

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Hybrid extracellular matrix design for cartilage‐mediated bone regeneration

Mikael

Nukavarapu

2017

J Biomed Mater Res

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Recapitulating long bone repair through endochondral ossification (EO) is increasingly becoming a more popular approach. A successful EO Process depends greatly on the establishment of a healthy hypertrophic-cartilage template (HCT). The aim of this work is to design a hydrogel system, which closely mimics the extracellular matrix of HCT. We examined the combinatorial effect of two commonly used hydrogels for bone and cartilage regeneration strategies, hyaluronan (HA) and fibrin (FB), to induce HCT formation. Hydrogel combinations were evaluated using a clinically relevant cell source, human bone marrow mesenchymal stem cells (hBMSCs). The results establish that with increasing HA (50-90%) the chondrogenic and its subsequent hypertrophy trend improved, with 70:30 HA:FB combination showing the highest and most uniform expression of chondrogenic and hypertrophic stage specific markers. This combination also showed superior support for cell micro-aggregation and differentiation. Thus, 70:30 HA-FB matrix demonstrated a healthy formation of chondrogenic and hypertrophic stages with rich stage-specific ECM components. This study demonstrates that with the appropriate hydrogel design it is possible to develop effective tissue engineering therapies for bone defect repair and regeneration through endochondral ossification by establishing a healthy HCT. V C 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B:Appl Biomater, 106B: 300-309, 2018.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hybrid extracellular matrix design for cartilage‐mediated bone regeneration

Mikael

Nukavarapu

2017

J Biomed Mater Res

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“…Because of the HA scaffold, it can also serve as a joint lubricant, thus encompassing a dual protective function. Since the HASH macromolecule is not readily crosslinkable via previously employed chemical crosslinking techniques [34,38,39,40,45,21,22,46], it offers a non-gelling yet lubricious and radicalprotective material for intra-articular injection. However, if needed, its structure could further be chemically crosslinked via other crosslinking strategies (i.e., divinyl sulfone or intramolecular esterification crosslinking) [58,59] that would preserve the availability of the freeradical scavenging thiol functionalities.…”

Section: Discussionmentioning

confidence: 99%

“…Thiolated HA derivatives were previously synthesized in our laboratory via hydrazide chemistry [34,38,39,40,45,21,22,46]. This strategy targeted the glucuronic acid (GlcA) residues of GAG disaccharide units.…”

Section: Synthesis and Characterization Of Ha-sulfhydryl (Hash)mentioning

confidence: 99%

“…Following reduction, these strategies yielded macromonomeric polythiols that could be crosslinked oxidatively via disulfide bonds or with bivalent electrophiles via thio-ether bonds, to obtain hydrogels and sponges [21,22]. A variety of medical and tissue engineering applications were successfully demonstrated by using thiol-modified crosslinked HA composites [34,37,35,38,39,40,41].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis, characterization and chondroprotective properties of a hyaluronan thioethyl ether derivative

2008

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Hyaluronan (HA), a non-sulfated glycosaminoglycan, is widely used in the clinic for viscosurgery, viscosupplementation, and treatment of osteoarthritis. Four decades of chemical modification of HA have generated derivatives in which the biophysical and biochemical properties, as well as the rates of enzymatic degradation in vivo have been manipulated and tailored for specific clinical needs. One earlier modification adds multiple thiol groups to HA through hydrazide linkages, leading to a readily crosslinkable material for adhesion prevention and wound healing. We now describe the synthesis and chemical characterization of a novel thioethyl ether derivative of HA, HA-sulfhydryl (HASH), with a minimal tether between the HA and the thiol group. Unlike earlier thiol-modified HA derivatives, HASH cannot be readily crosslinked to form a hydrogel using either oxidative or bivalent electrophilic conditions, thus offering a unique polymeric polythiol that remains soluble. Moreover, HASH showed no cytotoxicity towards primary human fibroblasts and reduced the apoptosis rates of primary chondrocytes exposed to hydrogen peroxide in vitro. These properties foreshadow the clinical potential of HASH to moderate inflammation and to act as a chondroprotective agent in vivo.

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Feasibility of First Injection of Autologous Adipose Tissue–Derived Stromal Vascular Fraction in Human Scarred Vocal Folds

Mattei

Bertrand

Jouve

et al. 2020

JAMA Otolaryngol Head Neck Surg

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IMPORTANCE Patients with scarred vocal folds, whether congenitally or after phonosurgery, often exhibit dysphonia that negatively affects daily life and is difficult to treat. The autologous adipose tissue-derived stromal vascular fraction (ADSVF) is a readily accessible source of cells with angiogenic, anti-inflammatory, immunomodulatory, and regenerative properties. OBJECTIVE To evaluate the feasibility and tolerability of local injections of autologous ADSVF in patients with scarred vocal folds. DESIGN, SETTING, AND PARTICIPANTS CELLCORDES (Innovative Treatment for Scarred Vocal Cords by Local Injection of Autologous Stromal Vascular Fraction) is a prospective, open-label, single-arm, single-center, nonrandomized controlled trial with a 12-month follow-up and patient enrollment from April 1, 2016, to June 30, 2017. Eight patients with severe dysphonia attributable to vocal fold scarring associated with a congenital malformation or resulting from microsurgical sequelae (voice handicap index score >60 of 120) completed the study. Data analysis was performed from September 1, 2018, to January 1, 2019. INTERVENTIONS Injection of ADSVF into 1 or 2 vocal folds. MAIN OUTCOMES AND MEASURES The primary outcomes were feasibility and the number and severity of adverse events associated with ADSVF-based therapy. The secondary outcomes were changes in vocal assessment, videolaryngostroboscopy, self-evaluation of dysphonia, and quality of life at 1, 6, and 12 months after cell therapy. RESULTS Seven women and 1 man (mean [SD] age, 44.6 [10.4] years) were enrolled in this study. Adverse events associated with liposuction and ADSVF injection occurred; most of them resolved spontaneously. One patient received minor treatment to drain local bruising, and another experienced a minor contour defect at the liposuction site. At 12 months, the voice handicap index score was improved in all patients, with a mean (SD) improvement from baseline of 40.1 (21.5) points. Seven patients (88%) were considered to be responders, defined as improvement by 18 points or more in the voice handicap index score (the minimum clinically important difference). CONCLUSIONS AND RELEVANCE The findings suggest that autologous ADSVF injection in scarred vocal folds is feasible and tolerable. The findings require confirmation in a randomized clinical trial with a larger population.

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Biocompatibility and stability of disulfide-crosslinked hyaluronan films

Cited by 117 publications

References 36 publications

Hybrid extracellular matrix design for cartilage‐mediated bone regeneration

Hybrid extracellular matrix design for cartilage‐mediated bone regeneration

Synthesis, characterization and chondroprotective properties of a hyaluronan thioethyl ether derivative

Feasibility of First Injection of Autologous Adipose Tissue–Derived Stromal Vascular Fraction in Human Scarred Vocal Folds

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