Biocompatibility and mineralization potential of new calcium silicate cements

Kim, Byurira; Lee, Yong-Hyuk; Kim, Ik-Hwan; Lee, Ko Eun; Kang, Chung-Min; Lee, Hyo-Seol; Choi, Hyung Jun; Cheon, Kyounga; Song, Je Seon; Shin, Young Goo

doi:10.1016/j.jds.2022.10.004

Cited by 2 publications

(1 citation statement)

References 35 publications

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“…It is important to understand how different materials interact with our dental tissues and cells. As reported by Kim et al [46], stem cells from human exfoliated deciduous teeth and human dental pulp stem cells had significantly low ALP activity after exposure to each material compared with the controls (i.e., cells cultured with osteogenic media). An elevated level of calcium release was found in all calcium silicate cements.…”

Section: Discussionsupporting

confidence: 65%

Assessment of pH Value and Release of Calcium Ions in Calcium Silicate Cements: An In Vitro Comparative Study

Herrera-Trinidad,

Molinero-Mourelle,

Fonseca

et al. 2023

Materials

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The goal of this study was to evaluate the pH and the release of calcium from four calcium-silicate-based cements. Methods: Four materials were tested (ProClinic MTA; Angelus MTA; ProRoot MTA; Biodentine). The palatal canal root of acrylic upper molars was filled with each cement. Afterwards, they were set in phosphate-buffered saline. Measurements were taken by atomic adsorption spectroscopy (AAS) at 3, 24, 72, 168, 336, 672, and 1008 h. The pH was measured at the same timepoints. Kruskal–Wallis tests were carried out in each period, as the Kolmogorov–Smirnov and Shapiro–Wilk tests showed no parametric results. Results: Significant differences (p < 0.05) in calcium release were found at the 3-, 24-, and 72-hour evaluations. All of the analyzed groups presented a release of calcium ions up to 168 h, and the general tendency was to increase up to 672 h, with a maximum release of 25.45 mg/g in the ProRoot group. We could only observe significant differences (p < 0.05) in pH value over 168 h between the Biodentine (7.93) and Angelus MTA (7.31) groups. Conclusions: There were significant differences (p < 0.05) in calcium release. Nevertheless, no significant differences (p > 0.05) in the pH values were found at the studied timepoints, except for the values at 168 h.

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Section: Discussionsupporting

confidence: 65%

Assessment of pH Value and Release of Calcium Ions in Calcium Silicate Cements: An In Vitro Comparative Study

Herrera-Trinidad,

Molinero-Mourelle,

Fonseca

et al. 2023

Materials

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Evaluation of the genotoxicity, cytotoxicity, and bioactivity of calcium silicate-based cements

Esen,

Guven,

Seyhan

et al. 2024

BMC Oral Health

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Background As calcium silicate-based cements (CSCs) have found success in various vital pulp therapy applications, several new CSC products have emerged. This study aimed to assess the genotoxicity, cytotoxicity, and bioactivity of four CSCs by comparing the newly introduced materials Bio MTA+ and MTA Cem with previously studied materials, Biodentine and NeoMTA. Methods Genotoxicity was evaluated using the micronucleus (MN) assay in human peripheral blood lymphocyte cells, measuring MN frequency and nuclear division index (NDI). Cytotoxicity was assessed in human dental pulp stem cells through the Water-Soluble Tetrazolium Salt-1 (WST-1) colorimetric assay. Bioactivity was determined by ELISA, measuring the levels of angiogenic and odontogenic markers (BMP-2, FGF-2, VEGF, and ALP). Statistical analyses included ANOVA, Dunnet and Sidak tests, and Wald chi-square test. (p < .05). Results The MN frequency in the groups was significantly lower than that in the positive control group (tetraconazole) (p < .05). NDI values decreased with increasing concentration (p < .05). Bio MTA+ and NeoMTA showed decreased cell viability at all concentrations in 7-day cultures (p < .01). All materials increased BMP-2, FGF-2, and VEGF levels, with Biodentine and NeoMTA showing the highest levels of BMP-2 and FGF-2 on day 7. Biodentine displayed the highest VEGF levels on day 7. Biodentine and NeoMTA groups exhibited significantly higher ALP activity than the Bio MTA+ and MTA Cem groups by day 7. Conclusion Bio MTA+ and MTA Cem demonstrated no genotoxic or cytotoxic effects. Moreover, this study revealed bioactive potentials of Bio MTA+ and MTA Cem by enhancing the expression of angiogenic and osteogenic growth factors.

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Biocompatibility and mineralization potential of new calcium silicate cements

Cited by 2 publications

References 35 publications

Assessment of pH Value and Release of Calcium Ions in Calcium Silicate Cements: An In Vitro Comparative Study

Assessment of pH Value and Release of Calcium Ions in Calcium Silicate Cements: An In Vitro Comparative Study

Evaluation of the genotoxicity, cytotoxicity, and bioactivity of calcium silicate-based cements

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