Biocompatibility and degradation characteristics of PLGA-based electrospun nanofibrous scaffolds with nanoapatite incorporation

Ji, Wei; Yang, Fang; Seyednejad, Hajar; Chen, Zhi; Hennink, Wim E.; Anderson, James M.; Beucken, Jeroen J.J.P. van den; Jansen, John A.

doi:10.1016/j.biomaterials.2012.06.018

Cited by 150 publications

(106 citation statements)

References 27 publications

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“…The decrease in the pH (acidic environment) during scaffold degradation leads to the agglomeration of the particles, which immobilizes them and prevents adverse effects commonly induced by nanoscale materials. The fast degradation might be considered an undesirable feature in theory, since previous studies have shown that the slower the degradation, the better the host response, [ 28 ] but in fact this was not seen in our study, probably attributed to nDP and BMP-2 modifi cations. Although the nDP and nDP+BMP-2 scaffolds that degraded faster had elevated mRNA levels of proinfl ammatory markers relative to PLCL at week 1, the infl ammatory cells present histologically in the site, particularly chronic infl ammation cells, were signifi cantly less at the later time points.…”

Section: Discussioncontrasting

confidence: 64%

“…The presence and quality of fi brous capsules and the type and amounts of cells were randomly evaluated in six fi elds of vision (magnifi cation 400×) of the entire implant area of each section using a modifi ed scoring system. [ 28 ] Histopathological examination of tissue specimens was limited to the tissue inside the scaffold and that in direct contact with it, evaluating the presence of different types of cells: those involved in early responses (neutrophils and plasma cells) and those involved in late or chronic responses (lymphocytes and foreign body giant cells). The grading for histological scoring is summarized in with ≈6-8 µm pixel size, 50 kV, aluminum 0.5 mm fi lter, and 0.4° rotation step.…”

Section: Descriptive Semiquantitative Histological Evaluationmentioning

confidence: 99%

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In Vivo Host Response and Degradation of Copolymer Scaffolds Functionalized with Nanodiamonds and Bone Morphogenetic Protein 2

Suliman

Sun

Pedersen

et al. 2016

Adv Healthcare Materials

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The aim is to evaluate the effect of modifying poly[( L -lactide)-co-(ε-caprolactone)] scaffolds (PLCL) with nanodiamonds (nDP) or with nDP+physisorbed BMP-2 (nDP+BMP-2) on in vivo host tissue response and degradation. The scaffolds are implanted subcutaneously in Balb/c mice and retrieved after 1, 8, and 27 weeks. Molecular weight analysis shows that modifi ed scaffolds degrade faster than the unmodifi ed. Gene analysis at week 1 shows highest expression of proinfl ammatory markers around nDP scaffolds; although the presence of infl ammatory cells and foreign body giant cells is more prominent around the PLCL. Tissue regeneration markers are highly expressed in the nDP+BMP-2 scaffolds at week 8. A fi brous capsule is detectable by week 8, thinnest around nDP scaffolds and at week 27 thickest around PLCL scaffolds. mRNA levels of ALP, COL1α2, and ANGPT1 are signifi cantly upregulating in the nDP+BMP-2 scaffolds at week 1 with ectopic bone seen at week 8. Even when almost 90% of the scaffold is degraded at week 27, nDP are observable at implantation areas without adverse effects. In conclusion, modifying PLCL scaffolds with nDP does not aggravate the host response and physisorbed BMP-2 delivery attenuates infl ammation while lowering the dose of BMP-2 to a relatively safe and economical level.

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Section: Discussioncontrasting

confidence: 64%

Section: Descriptive Semiquantitative Histological Evaluationmentioning

confidence: 99%

In Vivo Host Response and Degradation of Copolymer Scaffolds Functionalized with Nanodiamonds and Bone Morphogenetic Protein 2

Suliman

Sun

Pedersen

et al. 2016

Adv Healthcare Materials

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“…No residual hydrogel was detected by MRI with in situ polymerized PFGdDTPA-Cy5.5 on the third week of the experiment. These results are consistent with previous studies that use other techniques to document how in vivo fate of biomaterial implants is dependent on the composition, dimensions, in situ environment, and degradation products (13,15,(26)(27)(28). In the context of hydrogel biomaterial implants specifically, the MRI results are in close agreement with the work of Artzi et al (15); they also showed that implant geometry significantly affects the in vivo degradation patterns of the implant.…”

Section: Significancesupporting

confidence: 91%

Using bimodal MRI/fluorescence imaging to identify host angiogenic response to implants

Berdichevski

Yameen

Dafni³

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Therapies that promote angiogenesis have been successfully applied using various combinations of proangiogenic factors together with a biodegradable delivery vehicle. In this study we used bimodal noninvasive monitoring to show that the host response to a proangiogenic biomaterial can be drastically affected by the mode of implantation and the surface area-to-volume ratio of the implant material. Fluorescence/MRI probes were covalently conjugated to VEGF-bearing biodegradable PEG-fibrinogen hydrogel implants and used to document the in vivo degradation and liberation of bioactive constituents in an s.c. rat implantation model. The hydrogel biodegradation and angiogenic host response with three types of VEGFbearing implant configurations were compared: preformed cylindrical plugs, preformed injectable microbeads, and hydrogel precursor, injected and polymerized in situ. Although all three were made with identical amounts of precursor constituents, the MRI data revealed that in situ polymerized hydrogels were fully degraded within 2 wk; microbead degradation was more moderate, and plugs degraded significantly more slowly than the other configurations. The presence of hydrogel degradation products containing the fluorescent label in the surrounding tissues revealed a distinct biphasic release profile for each type of implant configuration. The purported in vivo VEGF release profile from the microbeads resulted in highly vascularized s.c. tissue containing up to 16-fold more capillaries in comparison with controls. These findings demonstrate that the configuration of an implant can play an important role not only in the degradation and resorption properties of the materials, but also in consequent host angiogenic response.biomaterial scaffold | angiogenesis | hydrogel | contrast agents | tissue regeneration W ith progress in the field of regenerative medicine relying more on approaches that deliver cells and/or bioactive factors using minimally invasive procedures, functional donor-tohost integration remains a critical challenge. In this context, biodegradable hydrogel scaffolds provide certain advantages, such as excellent tissue compatibility, temporary protection from host inflammation, and controlled resorption based on cell-mediated degradation (1). Effective transport properties to and within an implanted hydrogel are critical in the design of cell-seeded scaffolds, where the delivery of nutrients and removal of waste products from regions deep within the implant can severely limit the use of a patch for only the smallest of grafts (i.e., <0.5 mm) (2-4). Therefore, a great deal of research in the field of regenerative medicine has been devoted to ameliorate the survival of the cells and tissues with implants that use proangiogenic factors.One strategy is to enhance the localized formation of vascularized networks to improve the perfusion of oxygen and nutrients to the intended region in the body (2, 4, 5). This process is regulated by a number of different growth factors that stimulate a complex angiogenic res...

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“…The specific structures-filopodia and lamellipodia-related to the cell motility of fibroblasts can be observed both from SEM and CLSM micrographs. The cells emit cytoplasmic process towards the fibers and neighboring cells, communicating with the surrounding micro-environment and neighboring cells and allowing the passage of messengers [35]. Thus, we can conclude that even with a high content of drug and the use of organic solvent during the electrospinning process, PGH30 has no negative effect on cell morphology, viability, and proliferation.…”

Section: Proliferation Of Cells On Pgh-mna Nanofiber Membranesmentioning

confidence: 82%

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Xue

Liu³

et al. 2014

Biomaterials

321

217

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Email address: sharell@126.com (R. Shi). AbstractInfection is the major reason for guided tissue regeneration/guided bone regeneration (GTR/GBR) membrane failure in clinical application. In this work, we developed GTR/GBR membranes with localized drug delivery function to prevent infection by electrospinning of poly(ε-caprolactone) (PCL) and gelatin blended with metronidazole (MNA). Acetic acid (HAc) was introduced to improve the miscibility of PCL and gelatin to fabricate homogeneous hybrid nanofiber membranes. The effects of the addition of HAc and the MNA content (0,1,5,10,20,30, and 40 wt.% of polymer) on the properties of the membranes were investigated. The membranes showed good mechanical properties, appropriate biodegradation rate and barrier function. The controlled and sustained release of MNA from the membranes significantly prevented the colonization of anaerobic bacteria. Cells could adhere to and proliferate on the membranes without cytotoxicity until the MNA content reached 30%.Subcutaneous implantation in rabbits for 8 months demonstrated that MNA-loaded membranes evoked a less severe inflammatory response depending on the dose of MNA than bare membranes. The biodegradation time of the membranes was appropriate for tissue regeneration. These results indicated the potential for using MNA-loaded PCL/gelatin electrospun membranes as anti-infective GTR/GBR membranes to optimize clinical application of GTR/GBR strategies.

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Biocompatibility and degradation characteristics of PLGA-based electrospun nanofibrous scaffolds with nanoapatite incorporation

Cited by 150 publications

References 27 publications

In Vivo Host Response and Degradation of Copolymer Scaffolds Functionalized with Nanodiamonds and Bone Morphogenetic Protein 2

In Vivo Host Response and Degradation of Copolymer Scaffolds Functionalized with Nanodiamonds and Bone Morphogenetic Protein 2

Using bimodal MRI/fluorescence imaging to identify host angiogenic response to implants

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

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