Biocide resistance of Candida and Escherichia coli biofilms is associated with higher antioxidative capacities

Leung, Carmen; Chan, Yohan; Samaranayake, LP; Seneviratne, CJ

doi:10.1016/j.jhin.2011.09.014

Cited by 37 publications

(26 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…20,27e29 A number of different approaches have been taken to quantify growth, including both direct microbial culture and indirect measures, such as live/ dead viability assays. 20,30e32 Finally, different approaches to compare susceptibility in planktonic culture and biofilms have included measuring the amount of biocide required to inhibit growth [minimum inhibitory concentration (MIC)] or kill cells [minimum bactericidal concentration (MBC)], 7,18,20,33 or measuring the survival time at a given concentration of biocide; 20,32,34,35 this makes comparison of studies difficult.…”

Section: Biocide Susceptibilitymentioning

confidence: 99%

“…and two Escherichia coli strains to sodium hypochlorite, ethanol, hydrogen peroxide and iodine. 20 Strains were tested in planktonic culture, as attached cells and as biofilms in microtitre plates. Whilst susceptibility varied by organism and biocide, biofilms were less susceptible than attached cells, which were less susceptible than planktonic cells.…”

Section: Biocide Susceptibilitymentioning

confidence: 99%

“…42 Other studies have not identified reduced susceptibility for all biocides or organisms tested. 20,24,43,44 It is difficult to determine the relative importance of organism, biocide and testing conditions in these studies that found little or no reduced biocide susceptibility associated with biofilms.…”

Section: Biocide Susceptibilitymentioning

confidence: 99%

See 2 more Smart Citations

Surface-attached cells, biofilms and biocide susceptibility: implications for hospital cleaning and disinfection

Otter

Vickery

Walker

et al. 2015

Journal of Hospital Infection

200

114

View full text Add to dashboard Cite

Microbes tend to attach to available surfaces and readily form biofilms, which is problematic in healthcare settings. Biofilms are traditionally associated with wet or damp surfaces such as indwelling medical devices and tubing on medical equipment. However, microbes can survive for extended periods in a desiccated state on dry hospital surfaces, and biofilms have recently been discovered on dry hospital surfaces. Microbes attached to surfaces and in biofilms are less susceptible to biocides, antibiotics and physical stress. Thus, surface attachment and/or biofilm formation may explain how vegetative bacteria can survive on surfaces for weeks to months (or more), interfere with attempts to recover microbes through environmental sampling, and provide a mixed bacterial population for the horizontal transfer of resistance genes. The capacity of existing detergent formulations and disinfectants to disrupt biofilms may have an important and previously unrecognized role in determining their effectiveness in the field, which should be reflected in testing standards. There is a need for further research to elucidate the nature and physiology of microbes on dry hospital surfaces, specifically the prevalence and composition of biofilms. This will inform new approaches to hospital cleaning and disinfection, including novel surfaces that reduce microbial attachment and improve microbial detachment, and methods to augment the activity of biocides against surface-attached microbes such as bacteriophages and antimicrobial peptides. Future strategies to address environmental contamination on hospital surfaces should consider the presence of microbes attached to surfaces, including biofilms.

show abstract

Section: Biocide Susceptibilitymentioning

confidence: 99%

Section: Biocide Susceptibilitymentioning

confidence: 99%

See 1 more Smart Citation

Surface-attached cells, biofilms and biocide susceptibility: implications for hospital cleaning and disinfection

Otter

Vickery

Walker

et al. 2015

Journal of Hospital Infection

200

114

View full text Add to dashboard Cite

show abstract

“…Adhesion of microbial cells is linked with cell phenotype variations, which are usually manifested by new metabolic pathways, enhanced virulence and, as in the basis of this work, increased resistance to antimicrobial compounds (Nikolaev and Plakunov 2007). Biofilm resistance to various types of antimicrobial agents including biocides, antibiotics, and antiseptics is well reported (Cordeiro et al 2015;Donlan and Costerton 2002;Gilbert et al 2002;Leung et al 2012;Liao et al 2015). Although this phenomenon was firstly described for bacterial biofilms, yeast infections caused by biofilms, including Candida and Trichosporon, were also reported to exhibit high drug resistance (Al-Fattani and Douglas 2006;d'Enfert 2006;Di Bonaventura et al 2006).…”

Section: Introductionmentioning

confidence: 85%

Synergistic action of amphotericin B and rhamnolipid in combination on Candida parapsilosis and Trichosporon cutaneum

et al. 2017

View full text Add to dashboard Cite

Emerging resistance of microbial cells towards antibiotics or disinfectants leads to an increase of nosocomial diseases, often biofilm related, and gives rise to the need for research for new antimicrobial substances. These studies are often focused on substances that are able to support the efficacy of currently used antimicrobials, reduce their required dosage and reduce the probability of resistance development. This work brings new insights into the in vitro interaction of rhamnolipid biosurfactant and amphotericin B against Candida sp. or Trichosporon sp.. The minimum inhibitory concentrations and fractional inhibitory concentration indexes for both planktonic and biofilm cells were determined by the chequerboard microdilution method. Combination effect of rhamnolipid and amphotericin B was observed for both yeasts. Synergy, defined as a fractional inhibitory concentration (FICi) index of 0.50, was observed in both planktonic and biofilm cells of Trichosporon cutaneum. For C. parapsilosis, synergistic effect (FICi 0.5) was observed for planktonic cells, and additive effect (FICi 0.8) for biofilm cells. The influence of concentrations established as FICi on biofilm formation and susceptibility was studied by light microscopy. The highest inhibition (90% colonized area reduction) of initial adhesion of C. parapsilosis was observed when biofilm FIC was applied.

show abstract

“…With the rise of multidrug resistant bacterial pathogens, and their clonal expansion within biofilm, their control became a challenge for clinicians and associated healthcare providers. Most of the currently available biocidal agents are either highly cytotoxic or not efficient in complete inactivation of pathogen embedded in biofilm or their prevention biofilm [5]- [7]. Many antimicrobial agents in their sublethal as well as optimal doses selected resistant mutants, and thus failed to efficient eradication or inhibition of such pathogen, leaving a clear need for antibiofilm agents that can efficiently inhibit biofilm, and inactivate embedded pathogens from formed biofilms.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Biofilms by Non-Thermal Plasma Treated Novel Solutions

Ercan¹,

Joshi²,

Yost³

et al. 2014

AiM

View full text Add to dashboard Cite

Biofilms act as a reservoir of infection, and periodically release cells in vicinity that are capable of developing new biofilm colonies and disseminate infection. Many chronic bacterial infections are serious that are associated with biofilms and have high morbidity and mortality, partly due to their higher resistance to antimicrobial agents, and partly due to lack of strong biocides which can efficiently treat and inhibit biofilm formation. We recently demonstrated that nonequilibrium non-thermal dielectric-barrier discharge plasma (Plasma) can also be applied to control pathogens via applying treated-liquids, and these liquids acquire broad-spectrum antimicrobial properties. In present studies we demonstrated a range of plasma-activated simple chemical solutions which significantly inhibited biofilm formation by multidrug-resistant bacterial pathogens. Plasma-activated methionine solution exhibited strong inhibitory activity against the biofilms of carbapenem-resistant Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus, metallo-β-lactamase (NDM1)-positive Klebsiella pneumoniae, and Enterococcus faecalis, and prevented the formation of biofilms by about 70% as compared to untreated controls in single exposure. In addition to inhibition of biofilm formation, a complete inactivation of biofilm-embedded bacterial cells was observed in less than 30 minute's exposure to candidate plasma-activated methionine solution. These findings suggest that plasma-activated solutions have a potential to prevent biofilm formation, and as biofilm inhibitor.

show abstract

Biocide resistance of Candida and Escherichia coli biofilms is associated with higher antioxidative capacities

Cited by 37 publications

References 23 publications

Surface-attached cells, biofilms and biocide susceptibility: implications for hospital cleaning and disinfection

Surface-attached cells, biofilms and biocide susceptibility: implications for hospital cleaning and disinfection

Synergistic action of amphotericin B and rhamnolipid in combination on Candida parapsilosis and Trichosporon cutaneum

Inhibition of Biofilms by Non-Thermal Plasma Treated Novel Solutions

Contact Info

Product

Resources

About