Biocidal Efficacy of Silver-Impregnated Contact Lens Storage Cases In Vitro

Dantam, Jaya; Zhu, Hua; Stapleton, Fiona

doi:10.1167/iovs.09-4809

Cited by 50 publications

(25 citation statements)

References 40 publications

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“…62 Fungi can be resistant to the activity of several contact lens multipurpose disinfecting solutions. 63 Furthermore, a recent study investigating in vitro antimicrobial activity of three commercially available silver impregnated contact lens cases revealed high activity against bacteria, but all the lens cases were essentially ineffective against C. albicans after 6, 10, and 24 hours, 64 and only one lens case showed limited activity (0.5 log) against Fusarium solanii. 43 In this study we have demonstrated that the melimine-coated lenses produced at least one log inhibition against both Candida and Fusarium strains, indicating the possibility of controlling colonization of lens surfaces by fungi as well as bacteria.…”

Section: Discussionmentioning

confidence: 99%

Broad Spectrum Antimicrobial Activity of Melimine Covalently Bound to Contact Lenses

Dutta

Cole

Kumar

et al. 2013

Invest. Ophthalmol. Vis. Sci.

113

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PURPOSE. To develop a stable antimicrobial contact lens, which is effective against the International Organization for Standardization (ISO) panel microorganisms, Acanthamoeba castellanii and drug resistant strains of Pseudomonas aeruginosa and Staphylococcus aureus. METHODS.Melimine was covalently incorporated into etafilcon A lenses. The amount of peptide present on the lens surface was quantified using amino acid analysis. After coating, the heat stability (1218C), lens surface hydrophobicity (by captive bubble), and in vitro cytotoxicity to mouse L929 cells of the lenses were investigated. Antimicrobial activity against the micro-organisms was evaluated by viable plate count and fluorescence microscopy, measuring the proportion of cell death compared with control lenses with no melimine. RESULTS.The most effective concentration was determined to be 152 6 44 lg lens À1 melimine on the lens surface. After coating, lenses were relatively hydrophilic and were nontoxic to mammalian cells. The activity remained high after autoclaving (e.g., 3.1, 3.9, 1.2, and 1.0 log inhibition against P. aeruginosa, S. aureus, A. castellanii, and Fusarium solani, respectively). Fluorescence microscopy confirmed significantly reduced (P < 0.001) adhesion of viable bacteria to melimine contact lenses. Viable count confirmed that lenses were active against all the bacteria and fungi from the ISO panel, Acanthamoeba and gave at least 2 log inhibition against all the multidrug resistant S. aureus and P. aeruginosa strains.CONCLUSIONS. Melimine may offer excellent potential for development as a broad spectrum antimicrobial coating for contact lenses, showing activity against all the bacterial and fungal ISO panel microorganisms, Acanthamoeba, and antibiotic resistant strains of P. aeruginosa and S. aureus. (Invest Ophthalmol Vis Sci.

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Section: Discussionmentioning

confidence: 99%

Broad Spectrum Antimicrobial Activity of Melimine Covalently Bound to Contact Lenses

Dutta

Cole

Kumar

et al. 2013

Invest. Ophthalmol. Vis. Sci.

113

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“…For the three cases examined, Microblock, i-clean, and Nano-case, the antimicrobial activities were different (P Յ 0.001), with the Nanocase demonstrating the greatest activity, decreasing cell counts by 0.2 Ϯ 0.3 logs. Only the Microblock case showed silver release over 28 days (70).…”

Section: Resistance To Metalsmentioning

confidence: 99%

“…The biocidal efficacies of three silver-impregnated contact lens storage cases (Microblock, i-clean, and Nano-case) against S. maltophilia were tested (70). Case wells contained cell counts (10 3 to 10 6 CFU/ml) that were incubated for 6 to 24 h at 25°C.…”

Section: Resistance To Metalsmentioning

confidence: 99%

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

2012

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SUMMARY Stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. The increasing incidence of nosocomial and community-acquired S. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. S. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. Infections of S. maltophilia can occur in a range of organs and tissues; the organism is commonly found in respiratory tract infections. This review summarizes the current literature and presents S. maltophilia as an organism with various molecular mechanisms used for colonization and infection. S. maltophilia can be recovered from polymicrobial infections, most notably from the respiratory tract of cystic fibrosis patients, as a cocolonizer with Pseudomonas aeruginosa . Recent evidence of cell-cell communication between these pathogens has implications for the development of novel pharmacological therapies. Animal models of S. maltophilia infection have provided useful information about the type of host immune response induced by this opportunistic pathogen. Current and emerging treatments for patients infected with S. maltophilia are discussed.

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“…A comparison of organoselenium (1.0%) polypropylene contact lens case coupon versus the results of the best of the silver impregnated lens cases tested by 6 is the following (it should be noted that the silver data are for bacterial concentration in solution; we found the same concentration for our solution measurements as we did for the bacteria on the polypropylene, i.e., if there was no bacteria on the organoselenium polypropylene, there was none in solution): (1) MicroBlock (CIBA Vision) showed a reduction in P. aeruginosa of 2.4 logs, whereas organoselenium polypropylene contact lens case coupon was 7.3 logs (100%); S. marcescens reduction of 3.3 logs, whereas organoselenium polypropylene contact lens case coupon showed the Several stacks were acquired from each surface of the polymer, and the images were analyzed using the COMSTAT program.…”

Section: Discussionmentioning

confidence: 99%

“…Commercially available silver cases have been recently studied for their ability to inhibit bacterial growth in the solution stored in the case. 6 The authors found that the best reduction in bacterial binding from the different case types was the following: Staphylococcus aureus, 5.4 logs; Pseudomonas aeruginosa, 2.4 logs; Serratia marcescens, 3.3 logs; Delftia acidovorans, 2.8 logs; Fusarium solani, 0.5 logs; and Stenotrophomonas maltophilia only 0.2 logs. In an in vivo study where the cases used by patients were monitored, it was found that 70% of the cases were contaminated with biofilm irrespective of whether or not the case was impregnated with silver.…”

mentioning

confidence: 99%