Biochemistry of the White Blood Cell

Transport of Neutral and Dibasic Amino Acids by Human Leukocytes: Absence of Defect in Cystinuria*

“…VC -V, [1] where C, is the labeled amino acid concentration per milliliter of intracellular water, Rt the net radioactivity (counts per minute) of the packed cell pellet, Cm the labeled amino acid concentration per milliliter incubation medium, V. the trapped extracellular fluid volume, and Vt the total cell pellet fluid volume. V. was estimated with inulin-C" and may be expressed either in milliliters or in per cent of the packed cell volume.…”

“…Although this subject is of great interest to investigators of human physiology and disease, the lack of suitable and accessible human cell preparations for in vitro study has made progress in this field difficult. During the past decade methods have been devised for the isolation of human leukocytes from peripheral blood, and such cells have been used to study a wide range of physiologic and biochemical mechanisms (1). To date, however, leukocytes have been used sparingly in studies of ion or nonelectrolyte transport.…”

Leukocyte Alkaline Phosphatase: a Quantitative and Qualitative Study in Normal and Leukemic Cells

“…Both histochemical and biochemical methods have been employed in these investigations: recent reviews on this subject have been published by Perry [12] and Cline [4], The biochemical studies have, however, been carried out on heterogeneous preparations of leukocytes.…”

Characterization of Newborn Leukocyte Alkaline Phosphatase

“…It has been determined that leukocyte alkaline phosphatase (LAP; o-phosphoric monoester phosphohydrolase, EC 3.1.3.1) is first observed at the myelocyte stage and increases with cell maturation [11,23], Decreased activity of LAP has been reported in patients with chronic myelogenous leukemia [28]. Striking elevation of LAP has been observed in a variety of conditions which include polycythemia vera [16], Hodgkin's disease [11], Down's syndrome [1], bacterial infections [31], reactive granulo cytosis [28], stress conditions [29], pregnancy [25] and after the administra tion of various steroids including cortisone acetate and progesterone [15].…”