Characterization of Newborn Leukocyte Alkaline Phosphatase

Abstract

Leukocyte alkaline phosphatase (LAP) from cord blood was characterized by means of acrylamide gel disc electrophoresis, eneuraminidase sensitivity, heat inactivation, L-phenylalanine and L-homoargÍnine in hibition and EDTA inactivation. No differences were found between the LAP of cord blood and that of children in acrylamide gel disc electrophoresis, neuraminidase sensitivity, heat inactivation and L-homoarginine inhibition. Acrylamide gel disc electrophoresis of the agarose purified enzyme and EDTA inhibition studies suggest that the increase of LAP in cord blood depends on an increase of the catalytic efficiency of the enzyme.