Biochemistry of Suberization

Agrawal, Vishwanath Prasad; Kolattukudy, P. E.

doi:10.1104/pp.59.4.667

Cited by 44 publications

(6 citation statements)

References 18 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Supportively, the content of a-,v-dicarboxylic fatty acids has been found to decrease and that of v-hydroxy fatty acids to increase in the ace/ hth mutant, resulting in defects in cuticle development (Krolikowski et al, 2003;Kurdyukov et al, 2006). Meanwhile, the biochemical activity of v-hydroxyacid dehydrogenase and v-oxoacid dehydrogenase has been proven in epidermis of young Vicia faba leaves and potato (Solanum tuberosum) discs (Kolattukudy et al, 1975;Agrawal and Kolattukudy, 1977). Consistently, an abnormal anther cuticle and the significant reduction of C16:0 and C18:2 diacids were observed in the ipe1 mutant.…”

Section: Ipe1 Mediates the Lipid Metabolic Pathway In The Tapetummentioning

confidence: 98%

IRREGULAR POLLEN EXINE1 Is a Novel Factor in Anther Cuticle and Pollen Exine Formation

et al. 2016

View full text Add to dashboard Cite

ORCID IDs: 0000-0002-4656-3189 (H.S.); 0000-0003-0518-5924 (C.Z.); 0000-0001-9320-9628 (W.J.).Anther cuticle and pollen exine are protective barriers for pollen development and fertilization. Despite that several regulators have been identified for anther cuticle and pollen exine development in rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana), few genes have been characterized in maize (Zea mays) and the underlying regulatory mechanism remains elusive. Here, we report a novel male-sterile mutant in maize, irregular pollen exine1 (ipe1), which exhibited a glossy outer anther surface, abnormal Ubisch bodies, and defective pollen exine. Using map-based cloning, the IPE1 gene was isolated as a putative glucose-methanolcholine oxidoreductase targeted to the endoplasmic reticulum. Transcripts of IPE1 were preferentially accumulated in the tapetum during the tetrad and early uninucleate microspore stage. A biochemical assay indicated that ipe1 anthers had altered constituents of wax and a significant reduction of cutin monomers and fatty acids. RNA sequencing data revealed that genes implicated in wax and flavonoid metabolism, fatty acid synthesis, and elongation were differentially expressed in ipe1 mutant anthers. In addition, the analysis of transfer DNA insertional lines of the orthologous gene in Arabidopsis suggested that IPE1 and their orthologs have a partially conserved function in male organ development. Our results showed that IPE1 participates in the putative oxidative pathway of C16/C18 v-hydroxy fatty acids and controls anther cuticle and pollen exine development together with MALE STERILITY26 and MALE STERILITY45 in maize.Male sterility is a common biological phenomenon in plants and widely used in the production of hybrid seeds, which can reduce costs and enhance seed purity (Tester and Langridge, 2010). According to inheritance or origin, male sterility includes three types: genic male sterility, cytoplasmic male sterility, and cytoplasmicgenic male sterility (Rhee et al., 2015). The generation of mature pollen grains relies on anther development. The start of anther formation occurs in differentiated flower tissues (floral meristem), which consist of three histogenic layers: L1, L2, and L3. After continuous cell division and differentiation, L1 forms the epidermis and the L3 layer develops into the stomium and vascular bundles. L2 is the most important layer; it undergoes a series of periclinal and anticlinal divisions and eventually grows into the endothecium, the middle layer, the tapetum, and the pollen mother cells. When anther morphogenesis is completed, the anther has centrally localized pollen mother cells enclosed by four somatic layers, which are, from the surface to the interior, the epidermis, endothecium, middle layer, and tapetum. Then, the pollen mother cells undergo meiosis and mitosis, resulting in trinucleate pollen grains, and the endothecium, middle layer, and tapetum are gradually degraded (Goldberg et al., 1993(Goldberg et al., , 1995Ma, 2005).The anther cuticle and poll...

show abstract

Section: Ipe1 Mediates the Lipid Metabolic Pathway In The Tapetummentioning

confidence: 98%

IRREGULAR POLLEN EXINE1 Is a Novel Factor in Anther Cuticle and Pollen Exine Formation

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Such extracts catalyzed a stepwise oxidation of co-hydroxypalmitic acid first to the co-oxo acid and subsequently to the dicarboxylic acid (Agrawal and Kolattukudy, 1977). Such extracts catalyzed a stepwise oxidation of co-hydroxypalmitic acid first to the co-oxo acid and subsequently to the dicarboxylic acid (Agrawal and Kolattukudy, 1977).…”

Section: Enzymes Involved In the Biosynthesis Of Cutin And Suberinmentioning

confidence: 99%

Cutin, Suberin, and Waxes

Kolattukudy¹

1980

Lipids: Structure and Function

160

130

View full text Add to dashboard Cite

“…The most obvious general result of CYP86A33 silencing was the reduction by 60% of total aliphatic suberin affecting all monomers, but first and foremost C18:1 v-hydroxyacid (70% decrease) and a,v-diacid (90% decrease), which are the main monomers in aliphatic potato suberin. This fact, together with a marked drop in C20:0 v-hydroxyacid and C18:0 and C20:0 a,v-diacid, emphasizes the importance of CYP86A33 for v-hydroxylation in periderm, taking into account that v-hydroxyacids are precursors for the synthesis of a,v-diacids (Agrawal and Kolattukudy, 1977;Dean and Kolattukudy, 1977). The very minor increases in C22 and C24 v-hydroxyacid and a,v-diacid, observed only in 21-d-stored tubers, were not sufficient to compensate for the drop in v-functionalized fatty acids.…”

Section: Significance Of V-functional Fatty Acids In Suberin Biosynthmentioning

confidence: 99%

CYP86A33-Targeted Gene Silencing in Potato Tuber Alters Suberin Composition, Distorts Suberin Lamellae, and Impairs the Periderm's Water Barrier Function

et al. 2008

View full text Add to dashboard Cite

Suberin is a cell wall lipid polyester found in the cork cells of the periderm offering protection against dehydration and pathogens. Its biosynthesis and assembly, as well as its contribution to the sealing properties of the periderm, are still poorly understood. Here, we report on the isolation of the coding sequence CYP86A33 and the molecular and physiological function of this gene in potato (Solanum tuberosum) tuber periderm. CYP86A33 was down-regulated in potato plants by RNA interference-mediated silencing. Periderm from CYP86A33-silenced plants revealed a 60% decrease in its aliphatic suberin load and greatly reduced levels of C18:1 v-hydroxyacid (approximately 70%) and a,v-diacid (approximately 90%) monomers in comparison with wild type. Moreover, the glycerol esterified to suberin was reduced by 60% in the silenced plants. The typical regular ultrastructure of suberin, consisting of dark and light lamellae, disappeared and the thickness of the suberin layer was clearly reduced. In addition, the water permeability of the periderm isolated from CYP86A33-silenced lines was 3.5 times higher than that of the wild type. Thus, our data provide convincing evidence for the involvement of v-functional fatty acids in establishing suberin structure and function.

show abstract

Biochemistry of Suberization

Abstract: This c-hydroxyacid dehydrogenase activity, located largely in the 100,000g supernatant fraction, has a pH optimum of 9.5. It (11,12,14). As

Cited by 44 publications

References 18 publications

IRREGULAR POLLEN EXINE1 Is a Novel Factor in Anther Cuticle and Pollen Exine Formation

IRREGULAR POLLEN EXINE1 Is a Novel Factor in Anther Cuticle and Pollen Exine Formation

Cutin, Suberin, and Waxes

CYP86A33-Targeted Gene Silencing in Potato Tuber Alters Suberin Composition, Distorts Suberin Lamellae, and Impairs the Periderm's Water Barrier Function

Contact Info

Product

Resources

About