Biochemistry of filamentous fungi. V. Endogenous respiration during concurrent metabolism of exogenous substrates

Blumenthal, Harold J.; Koffler, Henry; Heath, Edward C.

doi:10.1002/jcp.1030500310

Cited by 17 publications

(7 citation statements)

References 39 publications

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“…It was found that the data fitted this assumption only when corrected for a continuing endogenous metabolism. In the case of ethanol, this criterion did not1permit a decision as to whether or not the endogenous metabolism continued, Blumenthal, Koffler & Heath (1957) showed that the manometric method described above and measurements based on release of radioactive carbon dioxide from l*C-labelled cells may lead to apparently contradictory conclusions concerning the effect of exogenous substrates on endogenous metabolism. It seemed desirable, therefore, to compare results obtained by the tracer method with our earlier respirometric studies.…”

Section: Resultsmentioning

confidence: 99%

The Endogenous Metabolism of Euglena gracilis

Danforth¹,

Wilson²

1961

Journal of General Microbiology

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SUMMARYThe endogenous respiratory rate of non-photosynthetic Euglena gracilis var. bacillaris is remarkably constant under varied conditions of growth and incubation. After 00-90 min. of slightly more rapid respiration, the endogenous oxygen uptake averages about 6.5 pl. O,/million cells/ hr., and this rate can be maintained for at least 26 hr. The respiratory quotient of the endogenous metabolism is 1.0.Fluoroacetate inhibits endogenous oxygen consumption about 70 %. A variety of evidence suggests that the polysaccharide paramylum is the major endogenous substrate.Tracer experiments indicate that the endogenous metabolism continues during the oxidation of exogenous substrates, but that the assimilated substrate mixes with, and dilutes the endogenous reserves. The time-course of this dilution process indicates that the most recently assimilated reserves are the first to be oxidized.

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Section: Resultsmentioning

confidence: 99%

The Endogenous Metabolism of Euglena gracilis

Danforth¹,

Wilson²

1961

Journal of General Microbiology

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“…It follows, therefore, that the status of the endogenous respiration may differ with each exogenous substrate and so no one calculation can be used for correcting the values obtained when resting cells act on a variety of substrates in an individual Warburg run. This problem was recognized by Blumenthal et al (1957) and discussed by them at some length. The present work was undertaken in anl effort to determine the cellular constituents which serve as reserve materials and which are utilized when resting cells of P. aeruginosa respire endogenously.…”

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confidence: 99%

ENDOGENOUS RESPIRATION OF PSEUDOMONAS AERUGINOSA

1960

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“…Assimilation of added glucose by M. gypseum was found to be 47%. It was of interest to note that this was considerably lower than the 83 and 74% reported by others for P. chrysogenium (3) and Nocardia corralina (13). Of the glucose assimilated, 50% was soluble in hot trichloroacetic acid and hot NaOH.…”

Section: Discussionmentioning

confidence: 61%

“…Similar enzymes have been demonstrated in M. canis (7). Blumenthal (2,3) investigated the problem of endogenous reserves in Penicillium chrysogenum and Neurospora crassa, and found the response of endogenous metabolism to the concomitant metabolism of an exogenous substrate to be a complex phenomenon influenced by age of cells, various environmental conditions, and substrate. Neither glucose nor acetate suppressed the endogenous respiration of P. chrysogenum grown in glucose medium.…”

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confidence: 99%

Oxidative Metabolism of Dermatophytes

McBride¹,

Stock²

1966

Applied Microbiology

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A method for preparing young, actively respiring dermatophyte myceha was obtained through the use of concentrated spore inocula and short growth periods in static culture. These hyphal elements were uniform in appearance, and vacuoles were absent. Concentrated mycelial suspensions were obtained which could be transferred easily and accurately. Glucose stimulated oxygen uptake in young mycelia which had been grown in a medium with low carbohydrate content. The level of endogenous respiration was affected by exogenous glucose only when this substrate stimulated oxygen uptake by less than 14%. Low nicotinamide adenine dinucleotide phosphate (NADP) dehydrogenase activity was noted in microconidia which have a low endogenous Qo2 value, whereas the activity of this enzyme was greater in macroconidia and mycelia which possess higher endogenous Qo2 values. Microsporum gypseum oxidizes 50% of exogenous glucose and assimilates the remainder. A large percentage of this substrate was assimilated into nitrogenous substances.

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Biochemistry of filamentous fungi. V. Endogenous respiration during concurrent metabolism of exogenous substrates

Abstract: 471 % SUBSTRATE ACT' P Y 0 2 COe TIME 06' PM 002 % SUBSTRATE (CPM/PM 0) CPM % ACT. RELEASED ACCOUNTED FOR 2 CONSUMED ACCOUNTED FOR REOOV.

Cited by 17 publications

References 39 publications

The Endogenous Metabolism of Euglena gracilis

The Endogenous Metabolism of Euglena gracilis

ENDOGENOUS RESPIRATION OF PSEUDOMONAS AERUGINOSA

Oxidative Metabolism of Dermatophytes

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