Oxidative Metabolism of Dermatophytes

Abstract

A method for preparing young, actively respiring dermatophyte myceha was obtained through the use of concentrated spore inocula and short growth periods in static culture. These hyphal elements were uniform in appearance, and vacuoles were absent. Concentrated mycelial suspensions were obtained which could be transferred easily and accurately. Glucose stimulated oxygen uptake in young mycelia which had been grown in a medium with low carbohydrate content. The level of endogenous respiration was affected by exogenous glucose only when this substrate stimulated oxygen uptake by less than 14%. Low nicotinamide adenine dinucleotide phosphate (NADP) dehydrogenase activity was noted in microconidia which have a low endogenous Qo2 value, whereas the activity of this enzyme was greater in macroconidia and mycelia which possess higher endogenous Qo2 values. Microsporum gypseum oxidizes 50% of exogenous glucose and assimilates the remainder. A large percentage of this substrate was assimilated into nitrogenous substances.

ANALISIS FENOTIPICO DEL COMPLEX Trichophyton rubrum EN LOS MEDIOS DE SABOURAUD, LACTRIMEL, Y LA TECNICA DE FISCHER & KANE: UNA EXPERIENCIA REGIONAL

“…La producción de estos metabolitos secundarios observados como compuestos coloreados en los cultivos, cumplirían una acción preventiva limitando la existencia de competidores bacterianos presentes en la piel y en las uñas (Summerbell, 2000). La glucosa, estimula la incorporación de oxígeno, actividad asociada a la enzima deshidrogenasa adenina dinucleótido fosfato (NADP), que condiciona la actividad respiratoria endógena de los microconidios que se incrementan al disminuir la enzima, por el contrario cuando ésta aumenta su actividad repercute en la formación de macroconidios (Mc Bride & Stock, 1966).…”