Biochemistry of Blood Plasma and Some Parameters of Antioxidant Status in Rats with Polyhypovitaminosis of Varying Severity

Abstract

In rats with profound vitamin deficiency, blood plasma level of triglycerides significantly decreased by 1.6 times, potassium ions by 5%, uric acid by 23%, ALT and AST by 1.4 times, while the levels of glucose increased by 32%, iron by 31%, urea by 58%, and alkaline phosphatase by 19%. Plasma level of phosphorus tended to decrease and ionized calcium concentration tended to increase. Severe deficiency of all vitamins is accompanied by pronounced accumulation of MDA in the plasma and liver together with simultaneous increase in the level of coenzyme Q10 by 4.6 times and decrease in vitamin C content by 21.4% in the rat liver compared to the control. It was found that severe combined deficiency of vitamins for 4 weeks produced considerable multidirectional alterations in diagnostically important metabolic parameters in rat plasma.

Subacute Oral Administration of Clinacanthus nutans Ethanolic Leaf Extract Induced Liver and Kidney Toxicities in ICR Mice

“…The blood samples collected in EDTA bottles were centrifuged for 15 min at 3000 rpm to obtain plasma for biochemical analyses [ 82 , 84 , 85 , 87 , 88 , 89 , 90 ]. The plasma was further analysed for urea, creatinine, creatinine kinase (CK), total protein (TP), albumin (ALB), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) using a fully automated clinical chemistry analyser (BioLis 24i Chemistry Analyzer, Tokyo, Japan) [ 7 , 84 , 86 ].…”

Toxicity Effects of Oral Administration of Clinacanthus nutans Ethanolic Leaf Extract on Blood, Liver and Kidneys of Mice

“…Plasma sample was obtained by centrifuging the anti-coagulated blood samples collected at 3000 rpm for 15 min for blood biochemical analyses [24][25][26][29][30][31][32] . The plasma was subsequently analysed using fully automated clinical chemistry analyser (BioLis 24i Chemistry Analyzer, Japan) for the concentration of urea, creatinine, Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Creatinine Kinase (CK), Total Protein (TP), Albumin (ALB) [10,24] .…”