Biochemical traits and proteomic changes in postharvest flowers of medicinal chrysanthemum exposed to enhanced UV-B radiation

Yao, Xiaogang; Chu, Jianzhou; Ma, Chunhui; Si, Chao; Li, Jigang; Shi, Xue; Liu, Chaonan

doi:10.1016/j.jphotobiol.2015.06.017

Cited by 13 publications

(2 citation statements)

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“…Most of the detected proteins were linked to carbohydrates, photosynthesis, energy metabolism, and cell structure, and the auxin-induced proteins PCNT115 and CmACO were tightly correlated with adventitious root formation, as supported by western blot experiments. A comparative proteomic analysis of postharvest medicinal chrysanthemum flowers under normal or UV-B radiation conditions allowed the detection of 43 differentially accumulated protein spots, some of which were identified as participating in photosynthesis, respiration, and defense 149 . The primary drawbacks of 2-DE are its low repeatability and resolution.…”

Section: Omics Technologiesmentioning

confidence: 99%

Current achievements and future prospects in the genetic breeding of chrysanthemum: a review

et al. 2019

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Chrysanthemum (Chrysanthemum morifolium Ramat.) is a leading flower with applied value worldwide. Developing new chrysanthemum cultivars with novel characteristics such as new flower colors and shapes, plant architectures, flowering times, postharvest quality, and biotic and abiotic stress tolerance in a time- and cost-efficient manner is the ultimate goal for breeders. Various breeding strategies have been employed to improve the aforementioned traits, ranging from conventional techniques, including crossbreeding and mutation breeding, to a series of molecular breeding methods, including transgenic technology, genome editing, and marker-assisted selection (MAS). In addition, the recent extensive advances in high-throughput technologies, especially genomics, transcriptomics, proteomics, metabolomics, and microbiomics, which are collectively referred to as omics platforms, have led to the collection of substantial amounts of data. Integration of these omics data with phenotypic information will enable the identification of genes/pathways responsible for important traits. Several attempts have been made to use emerging molecular and omics methods with the aim of accelerating the breeding of chrysanthemum. However, applying the findings of such studies to practical chrysanthemum breeding remains a considerable challenge, primarily due to the high heterozygosity and polyploidy of the species. This review summarizes the recent achievements in conventional and modern molecular breeding methods and emerging omics technologies and discusses their future applications for improving the agronomic and horticultural characteristics of chrysanthemum.

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Section: Omics Technologiesmentioning

confidence: 99%

Current achievements and future prospects in the genetic breeding of chrysanthemum: a review

et al. 2019

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“…Initially, morphological [ 14 , 15 ], cellular [ 16 ], and biochemical markers [ 12 , 17 ] were often used as the major detection methods to distinguish different chrysanthemum cultivars. All these methods are difficult to operate, and are susceptible to environmental and developmental factors, and to plant sample adulteration.…”

Section: Introductionmentioning

confidence: 99%

Development and Application of a Cultivar-Specific Sequence-Characterized Amplified Region (SCAR) Marker for the Detection of Chrysanthemum morifolium Ramat. ‘Daboju’

Cai

Gao

Zhang

et al. 2022

Plants

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Chrysanthemummorifolium Ramat. ‘Daboju’ is a C. morifolium cultivar with important ornamental and medicinal values, and is often used in the treatment of colds, blurred vision, dizziness, and itchy skin. As the morphological characteristics of C. morifolium ‘Daboju’ are very similar to those of other C. morifolium cultivars, they are often confused in practice. However, the medicinal value and practical use of C. morifolium depends on using the correct rapid and accurate identification of C. morifolium ‘Daboju’ and its differentiation from other, morphologically similar C. × morifolium cultivars. Twenty-one polymorphic start codon-targeted (SCoT) primers were amplified in 21 distinct C. morifolium cultivars. One cultivar-specific DNA marker was developed with the aim of the rapid and accurate identification of C. morifolium ‘Daboju’ and its differentiation from other, similar C. morifolium cultivars. Twenty-one polymorphic start codon-targeted (SCoT) primers were amplified in 21 distinct C. morifolium cultivars. One cultivar-specific 385-bp amplicon (named SCoT36-385), amplified only in C. morifolium ‘Daboju’ (and in all samples of this cultivar), was identified, cloned, and sequenced. Subsequently, a sequence-characterized amplified region (SCAR) marker (named DBJF/DBJR), generating a 360-bp amplicon, was developed from SCoT36-385 and tested for amplification in all 21 C. morifolium cultivars, ten C. morifolium ‘Daboju’ populations, and different simulated adulterations of ‘Daboju’ with other cultivars. The primers amplified the specific 360-bp-long DNA fragment in all the tested C. morifolium ‘Daboju’ samples but failed in the absence of ‘Daboju’. The detection limit of the SCAR primer pair (DBJF/DBJR) was 100 pg of DNA extracted from C. morifolium ‘Daboju’. Hence, this SCAR marker has a very high detection sensitivity, and can be used for accurate and rapid identification of C. morifolium ‘Daboju’. It can play an important role in ensuring the quality of medicinal preparations and protecting C. morifolium ‘Daboju’ germplasm resources in breeding programs and in identifying lines generated from this cultivar.

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Medicinal Plants Proteomics in Response to Abiotic Stresses

Alugoju¹,

Tencomnao²

2023

Medicinal Plants

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Biochemical traits and proteomic changes in postharvest flowers of medicinal chrysanthemum exposed to enhanced UV-B radiation

Cited by 13 publications

References 55 publications

Current achievements and future prospects in the genetic breeding of chrysanthemum: a review

Current achievements and future prospects in the genetic breeding of chrysanthemum: a review

Development and Application of a Cultivar-Specific Sequence-Characterized Amplified Region (SCAR) Marker for the Detection of Chrysanthemum morifolium Ramat. ‘Daboju’

Medicinal Plants Proteomics in Response to Abiotic Stresses

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