Development and Application of a Cultivar-Specific Sequence-Characterized Amplified Region (SCAR) Marker for the Detection of Chrysanthemum morifolium Ramat. ‘Daboju’

Abstract

Chrysanthemummorifolium Ramat. ‘Daboju’ is a C. morifolium cultivar with important ornamental and medicinal values, and is often used in the treatment of colds, blurred vision, dizziness, and itchy skin. As the morphological characteristics of C. morifolium ‘Daboju’ are very similar to those of other C. morifolium cultivars, they are often confused in practice. However, the medicinal value and practical use of C. morifolium depends on using the correct rapid and accurate identification of C. morifolium ‘Daboju’ and its differentiation from other, morphologically similar C. × morifolium cultivars. Twenty-one polymorphic start codon-targeted (SCoT) primers were amplified in 21 distinct C. morifolium cultivars. One cultivar-specific DNA marker was developed with the aim of the rapid and accurate identification of C. morifolium ‘Daboju’ and its differentiation from other, similar C. morifolium cultivars. Twenty-one polymorphic start codon-targeted (SCoT) primers were amplified in 21 distinct C. morifolium cultivars. One cultivar-specific 385-bp amplicon (named SCoT36-385), amplified only in C. morifolium ‘Daboju’ (and in all samples of this cultivar), was identified, cloned, and sequenced. Subsequently, a sequence-characterized amplified region (SCAR) marker (named DBJF/DBJR), generating a 360-bp amplicon, was developed from SCoT36-385 and tested for amplification in all 21 C. morifolium cultivars, ten C. morifolium ‘Daboju’ populations, and different simulated adulterations of ‘Daboju’ with other cultivars. The primers amplified the specific 360-bp-long DNA fragment in all the tested C. morifolium ‘Daboju’ samples but failed in the absence of ‘Daboju’. The detection limit of the SCAR primer pair (DBJF/DBJR) was 100 pg of DNA extracted from C. morifolium ‘Daboju’. Hence, this SCAR marker has a very high detection sensitivity, and can be used for accurate and rapid identification of C. morifolium ‘Daboju’. It can play an important role in ensuring the quality of medicinal preparations and protecting C. morifolium ‘Daboju’ germplasm resources in breeding programs and in identifying lines generated from this cultivar.

The genus Chrysanthemum: Phylogeny, biodiversity, phytometabolites, and chemodiversity

“…In a ISSR analysis ( Lyu et al, 2008 ), both Da Boju and Xiao Boju were closer to different cultivars of Hangju. A cultivar-specific sequence-characterized amplified region (SCAR) marker was developed to detect Da Boju ( Cai et al, 2022 ), which is morphologically similar to ornamental Xiaoju, and could be artificially evolved from Xiaoju.…”

Development of highly discriminatory SCoT- and CBDP-based SCAR fingerprint for authentication of Indian senna (Senna alexandrina Mill.) formerly Cassia angustifolia Vahl.)

“…de Wild) and its adulterant Polyalthia longifolia Benth ( Kumar, 2016 ), Artemisia , Phyllanthus , Panax ( Kiran et al., 2010 ), and Dimocarpus longan varieties ( Yang et al., 2013 ). Germplasm and cultivar-specific SCoT-based SCAR was identified in Dendrobium officinale ( Zheng et al., 2021 ) and Chrysanthemum morifolium Ramat ( Cai et al., 2022 ). Deng (2021) developed SCoT-based SCAR to detect smut resistance in sugarcane.…”