Biochemical study of DNA markers for Bacterial infection in bovine mastitis

Abstract

Mastitis is a multifactorial disease and very difficult to control. It results from injury, chemical irritation and infection caused by different bacterial species. Mastitis remains one of the most common economic problems of dairy industry worldwide as it is the most expensive disease of dairy animals resulting in the reduction of milk production and quality. These expenses in terms of reduction of production, discarding milk, drug therapy, veterinarian charges, culling of incurable animals and extrause of labor. Analysis of bacteriological examination of milk samples was made to identify the main etiological agents involved in the disease. The organisms were identified on the basis of their cultural, staining characteristics, biochemical reactions .and molecular detection. Milk sample of 23 cows, which were positive for California mastitis test, cultured for microbiological examination in the study period. Two bacterial species were isolated, Staphylococcus aureus (Staph. aureus) and E. coli bacterial isolates. The predominant isolated bacteria were Staph. aureus with isolation rate of 37.77% however E. coli was isolated with isolation rate of 13.33%). Serum alkaline phosphatase (ALP) enzyme and calcium levels were highly significant decreased while C-Reactive protein (CRP) titre and phosphorus levels were highly significant increased. Lactate dehydrogenase enzyme(LDH), Aspartate aminotransferase enzyme(AST), Gamma-glutamyl transferase enzyme(GGT), Albumin, sodium, potassium and chloride levels were nonsignificantly changed in serum of mastitic cows compared to healthy ones. While LDH, ALP and phosphorus levels were highly significant increase in milk of mastitic cows compared to that of healthy ones. However, the calcium level was high significantly decreased in mastitic cows compared to healthy ones. Molecular detection of Staph. aureus and E. coli isolates by PCR. The expected sizes of PCR products of Staph. aureus was (984bp), while that for E. coli were (662bp).

The Efficacy of Alcoholic Extracts of Morus Macroura (Mulberries), Lepidium Sativum (Garden Cress Seeds) and Diclazuril Against E. stiedae in Experimentally Infected Rabbits

“…Following treatment, the recovery included healing of damaged liver tissues and bile ducts (the primary site of infection) as well as normalization of many biochemical parameter levels, including liver enzymes and blood serum proteins following treatment. These results are similar to the findings of Abdel Maged et al (2013) and Çam et al (2008). In addition, Faris et al (2011) who documented that coccidiosis in kids caused a substantial increase in serum AST, ALT, urea, and creatinine while the current findings demonstrated that after treatment with herbal extracts, all biochemical were restored to normal levels, confirming their anti-coccidial efficacy and hepatoprotective actions.…”

Ameliorative effect of alcoholic extract of Glycyrrhiza glabra and diclazuril against experimental Eimeria stiedae infection in rabbits

“…While the improvement of blood serum enzymes in (G1, G2) could be attributed to the recovery of hepatic cells and the lining epithelium of bile ducts (the primary site of infection), inhibition of the parasite sexual stages development and normalization of many biochemical parameters' levels, including liver enzymes following pretreatment. These results are similar to that of Abdel Maged et al (2013) and Çam et al (2008). The current observations revealed that the recorded values of tested biochemical parameters were nearly similar to those of control non infected group after pretreatment by licorice extract which approve its anti-coccidial efficacy and hepato-protective effects.…”