Biochemical Responses of the Bivalve Mollusk Unio tumidus Inhabiting a Small Power Plant Reservoir on the Dniester River Basin, Ukraine

Khoma, Vira; Gnatyshyna, Lesya; Martinyuk, Viktoria; Rarok, Yulya; Mudra, A. Ye.; Stoliar, Оksana

doi:10.1007/s00128-020-02873-2

Cited by 4 publications

(3 citation statements)

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“…The chromatographic properties of MTs were also non-disturbed in the exposure to 80 nM of Rn alone and in combinations [10]. Only in the acute ex vivo exposure, the similar to utilized here about 40 nM concentration of glyphosate (Rn) caused the decrease of MTSH concentration (by ~ two times) in coordination with the depletion of total antioxidant activity [10,11,12]. However, the level of TBARS was not changed compared to control detecting the early stage of the injury.…”

Section: Resultsmentioning

confidence: 50%

“…1 and [10, 11]). When we related the effects induced by the concentration of Rn alone with the two times lower Rn concentration used in combined exposures, the decreased SOD but increased levels of GSH, MTSH and LPO were indicated [10][11][12]. Consequently, despite different responses of SOD, in the exposures to Rn, the same main role of MTSH was shown.…”

Section: Resultsmentioning

confidence: 93%

“…However, these unique features make them particularly sensitive to environmental perturbations resulting from global climate change and lead to their global decline [18]. In our previous studies, we reported high sensitivity of freshwater mussels to the local environmental peculiarities (even depending on the location of the power plant dam) [8,11]. In the subchronic exposure in vivo to Roundup alone (80 nM of glyphosate) and in combinations with pharmaceuticals and heating, particular toxicity of Roundup to the mussels has been shown [10,12].…”

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confidence: 94%

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Комбінована Дія Низької Концентрації Раундапу На Двостулкового Молюска Активує Тіолові Сполуки У Травній Залозі

Khoma

Gnatyshyna

Martinyuk

et al. 2020

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Glyphosate is one of most popular weed killers in the word. Its toxicity to aquatic organisms was investigated mostly in the acute high experimental exposures. The aim of this study was to evaluate the effect of low, 0.5 of Predicted No Effect Concentration (PNEC), of glyphosate in the combinations to freshwater bivalve mollusks.We treated the mussels Unio tumidus with glyphosatebased herbicide RoundupMAX (Rn, correspondent to 16.9 µg L-1 or 40 nM of glyphosate) in the combination with heating 25o C (RnT) or chlorpromazine (RnCpz, 18.0 µg L-1 or 56 nM of Cpz) and Cpz alone during 14 days. The responses of oxidative stress were evaluated in the digestive gland. The enzyme activities were changed only by the exposures to Rn (increase of superoxide dismutase) and Cpz (decrease of catalase), whereas the elevation of total glutathione (GSH) level was indicated in all exposures except Rn, and metallothonein-associated thiols (MTSH) – in all exposures except Cpz. Lipid peroxidation was increased in all exposures by 16.6% maximally. Total balance of antioxidants versus prooxidative changes was increased in all exposures contained Rn (by ~3 times in RnT-group) and decreased in the exposure to Cpz alone. Metallothionein chromatographyc features did not indicate substantial oxidative changes in all exposed groups. Hence, combine exposures distort prominently the oxidative stress responses to xenobiotics in the freshwater mussels even in low, nanomolar concentrations. The ability of Rn to induce MTSH seems to be the decisive input in the antioxidant defence at combine exposures.

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