Biochemical responses, morphometric changes, genotoxic effects and CYP1A expression in the armored catfish Pterygoplichthys anisitsi after 15 days of exposure to mineral diesel and biodiesel

Felício, Andréia Arantes; Parente, Thiago Estevam; Maschio, Lucilene Regina; Nogueira, Lílian; Venâncio, Larissa Paola Rodrigues; Rebelo, Mauro de Freitas; Schlenk, Daniel; Almeida, Eduardo Alves de

doi:10.1016/j.ecoenv.2015.01.034

Cited by 18 publications

(6 citation statements)

References 36 publications

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“…Fish handling was carried out in accordance with relevant guidelines and approved by the Ethics Committee, as described elsewhere (Felício et al, 2015). RNA was extracted using either Trizol (Invitrogen) or TRI Reagent (Life technologies) following manufacturer instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Tilapia, Oreochromis niloticus ) to biodiesel, showing no mortality upon exposure to 6.0 mL.L -1 during 15 days (Felício et al, 2015; Nogueira et al, 2011b). The molecular mechanisms underlying P. anisitsi resistance to organic xenobiotics have not been established.…”

Section: Introductionmentioning

confidence: 99%

“…However, the cytochrome P450 1A (CYP1A) from Pterigoplichthys spp. and some species of the closely related genus Hypostomus has been shown to possess amino acid substitutions that alter their substrate specificities, resulting in undetectable or extremely low ethoxyresorufin- O -deethylase (EROD) activity in the liver of these fishes (Felício et al, 2015; Nogueira et al, 2011a; Parente et al, 2015, 2014, 2011, 2009). Among Vertebrates, this is an aberrant phenotype of a crucial detoxification enzyme known to take part, for example, in the activation of pre-mutagenic toxins that could potentially be involved in the elevated resistance of P. anisitsi to biodiesel.…”

Section: Introductionmentioning

confidence: 99%

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The liver transcriptome of suckermouth armoured catfish (Pterygoplichthys anisitsi, Loricariidae): Identification of expansions in defensome gene families

Parente

Moreira

Magalhães

et al. 2017

Marine Pollution Bulletin

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Pterygoplichthys is a genus of related suckermouth armoured catfish native to South America that has invaded tropical and subtropical regions worldwide. Physiological features, including an augmented resistance to organic xenobiotics, may have aided their settlement in foreign habitats. The liver transcriptome of Pterygoplichthys anisitsi was sequenced and used to characterize the diversity of mRNAs potentially involved in the responses to natural and anthropogenic chemicals. In total, 66,642 transcripts were assembled. Among the identified defensome genes, cytochromes P450 (CYP) were the most abundant, followed by nuclear receptors (NR), sulfotransferases (SULT) and ATP binding cassette transporters (ABC). A novel expansion in the CYP2Y subfamily was identified, as well as an independent expansion of the CYP2AAs. Two expansions were also observed among SULT1. Thirty-two transcripts were classified into twelve subfamilies of NR, while 21 encoded ABC transporters. The diversity of defensome transcripts sequenced herein could contribute to this species resistance to organic xenobiotics.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The liver transcriptome of suckermouth armoured catfish (Pterygoplichthys anisitsi, Loricariidae): Identification of expansions in defensome gene families

Parente

Moreira

Magalhães

et al. 2017

Marine Pollution Bulletin

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“…The increase in EROD activity is possibly related to the transcriptional modulation of the CYP1A gene by the AhR receptor, suggesting a cellular biotransformation capacity of PAHs in fish tissues exposed to WSFDO (Timme-Laragy et al, 2007). However, a species of catfish exposed to diesel oil and also biodiesel increased EROD activities but CYP1AmRNA expression was unaltered after exposition (Felício et al, 2015). This lacking agreement was explained by the large periods of Fig.…”

Section: Laboratory Assaymentioning

confidence: 51%

“…Generally, antioxidant enzymes are less sensible to pollutants than Phase I and II biotransformation enzymes, however, the fact that CAT was not induced does not exclude reactive oxygen species (ROS) formation, which in turn may be metabolized by glutathione peroxidase (GPx) (Van Der Oost et al, 2003). Compared to the present study and species, the catfish species Pterygoplichtys anisitsi had similar behavior of EROD, CAT and GST when exposed not only to diesel oil, but also to biodiesel which has been produced or mixed to diesel oil in Brazil but has also harmful effects (Felício et al, 2015).…”

Section: Laboratory Assaymentioning

confidence: 71%

Effects of diesel oil and environmental quality on the enzymatic activities of a tropical estuarine catfish and implications for contamination assessment

Berenger¹,

Moraes²,

Oliveira³

et al. 2024

Ecotoxicol. Environ. Contam.

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The contamination of coastal areas by petroleum and its derivatives is a relevant issue based on large-scale production and consumption. This study presents field and laboratory surveys on the detoxification enzymes on the estuarine/coastal catfish Genidens genidens to understand the enzymatic responses to oil derivatives and environmental conditions. Field catalase (CAT) and glutathione S-tranferase (GST) measurements suggest oxidative stress and acting repair mechanisms in fishes, but were not induced by diesel oil as indicated by laboratory studies. On the other hand, higher ethoxyresorufin-O-deethylase (EROD) activities following higher polycyclic aromatic hydrocarbon (PAH) concentrations in the estuarine waters suggested fish responsiveness to oil contamination, mainly due to the fact that such EROD values were similar to the magnitude measured in fish during a 24-h exposure to diesel oil in the laboratory. However, other environmental estuarine conditions may also act on field EROD activities since PAHs concentrations in the water-soluble fraction of diesel oil (WSFDO) were higher than those measured in estuarine waters.

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Biomarkers of Exposure and Effect in Nile Tilapia (Oreochromis niloticus) Environmentally Exposed to Multiple Stressors in Egypt

Salem

Hagras

Elbaghdady

et al. 2021

Bull Environ Contam Toxicol

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Chemical pollution poses adverse effects on aquatic organisms, including altered gene expression. This study, therefore, investigated the hepatic expression of biomarkers of exposure and effect in Oreochromis niloticus inhabiting the drainage canal of a sugarbeet factory (an industrially polluted habitat) and the Nile River (a reference habitat). Compared to the Nile River, the drainage canal contained significantly elevated levels of heavy metals (Cd, Fe, Mn, Co, and Pb), nitrate, ammonia, nitrite, chemical oxygen demand, and biological oxygen demand. The liver of O. niloticus from the drainage canal accumulated significantly higher concentrations of the heavy metals. O. niloticus from the drainage canal had significantly higher hepatic expressions of genes related to oxidative stress (GSTa, GPx, and NRF2), metal toxicity (MT), endocrine disruption (Vtg), and hypoxia (HIF1a) and a significantly lower hepatic expression of the apoptosis-associated CAS gene. CYP1A (a detoxification biomarker) expression did not exhibit significant differences. These results provide insights into complex adaptive responses of O. niloticus to multiple chemicals and support the utilization of molecular biomarkers as warning signals for water pollution.

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Biochemical responses, morphometric changes, genotoxic effects and CYP1A expression in the armored catfish Pterygoplichthys anisitsi after 15 days of exposure to mineral diesel and biodiesel

Cited by 18 publications

References 36 publications

The liver transcriptome of suckermouth armoured catfish (Pterygoplichthys anisitsi, Loricariidae): Identification of expansions in defensome gene families

The liver transcriptome of suckermouth armoured catfish (Pterygoplichthys anisitsi, Loricariidae): Identification of expansions in defensome gene families

Effects of diesel oil and environmental quality on the enzymatic activities of a tropical estuarine catfish and implications for contamination assessment

Biomarkers of Exposure and Effect in Nile Tilapia (Oreochromis niloticus) Environmentally Exposed to Multiple Stressors in Egypt

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