The liver transcriptome of suckermouth armoured catfish (Pterygoplichthys anisitsi, Loricariidae): Identification of expansions in defensome gene families

Parente, Thiago Estevam; Moreira, Daniel A.; Magalhães, Maithê Gaspar Pontes; Andrade, Paula; Furtado, Carolina; Haas, Brian J.; Stegeman, John J.; Hahn, Mark E.

doi:10.1016/j.marpolbul.2016.12.012

Cited by 14 publications

(15 citation statements)

References 60 publications

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“…Therefore, there may be no zebrafish homologs of the higher vertebrate and Xenopus SULT1 subfamilies. Similar phylogenetic relationships were obtained in a catfish (Pterygoplichthys anisitsi) SULT1 family (Parente et al, 2017), where 10 SULT1 members clustered into two clades. One is more closely related to the human SULT1A subfamily, and the other is distant from any mammalian SULT1 subfamilies.…”

Section: Discussionsupporting

confidence: 66%

A prototype of the mammalian sulfotransferase 1 (SULT1) family in Xenopus laevis: molecular and enzymatic properties of XlSULT1B.S

2019

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The cytosolic sulfotransferase 1 (SULT1) proteins are a family of highly divergent proteins that show variable expansion in different species during vertebrate evolution. To clarify the evolutionary origin of the mammalian lineage of the SULT1 family, we compiled Xenopus laevis and X. tropicalis SULT1 (XSULT1) sequences from public databases. The XSULT1 family was found to comprise at least six subfamilies, which corresponded in part to five mammalian SULT1 subfamilies but only poorly to zebrafish SULT1 subfamilies. SULT1C was most highly expanded, and could be divided into at least five subfamilies. A cDNA for X. laevis SULT1B (XlSULT1B.S), a homolog of mammalian SULT1B1, was cloned and its recombinant protein was expressed in a bacterial system. XlSULT1B.S, unlike mammalian SULT1B1, was found to be a monomeric protein of ~34 kDa, and displayed sulfonating activity toward 2-naphthol and p-nitrophenol (pNP). However, we could not detect such sulfonating activity toward any endogenous compounds including thyroid hormones, steroid hormones and dopamine, despite the fact that X. laevis and Rana catesbeiana liver cytosols contained sulfonating activity toward most of these endogenous compounds. At optimum pH (6.4), the Michaelis-Menten constant (K m ) for pNP was two orders of magnitude greater in XlSULT1B.S (1.04 mM) than in the cytosol preparations (8-15 μM). Our results indicate that Xenopus possesses a prototype of the mammalian SULT1 family, and that XlSULT1B.S showed overall similarities in primary sequence to, and significant differences in molecular and enzymatic properties from, mammalian SULT1B1.

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Section: Discussionsupporting

confidence: 66%

A prototype of the mammalian sulfotransferase 1 (SULT1) family in Xenopus laevis: molecular and enzymatic properties of XlSULT1B.S

2019

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“…Here, the mitochondrial genome of P. disjunctivus is used to confirm our findings, as it was obtained using a combination of long and short PCR from DNA and sequenced using the Sanger method [ 6 ]. Additionally, this deletion is supported by the recently released mitochondrial genomes of Hypancistrus zebra ( Peckoltia -clade; KX611143.1) [ 11 ] and Pterygoplichthys anisitsi (Hypostomini; KT239003, KT239004, KT239005) [ 12 ].…”

Section: Discussionmentioning

confidence: 98%

“…Additionally, the sequences of the 13 protein-coding genes and the two ribosomal RNAs were used to test the currently accepted phylogenetic relationships among Loricariidae subfamilies. These resources complement our recent efforts [ 8 – 12 ], augmenting from four to 36 the number of nearly complete mitochondrial genomes available for Loricarioidei fishes.…”

Section: Introductionmentioning

confidence: 96%

Reducing the information gap on Loricarioidei (Siluriformes) mitochondrial genomics

et al. 2017

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BackgroundThe genetic diversity of Neotropical fish fauna is underrepresented in public databases. This distortion is evident for the order Siluriformes, in which the suborders Siluroidei and Loricarioidei share equivalent proportion of species, although far less is known about the genetics of the latter clade, endemic to the Neotropical Region. Recently, this information gap was evident in a study about the structural diversity of fish mitochondrial genomes, and hampered a precise chronological resolution of Siluriformes. It has also prevented molecular ecology investigations about these catfishes, their interactions with the environment, responses to anthropogenic changes and potential uses.ResultsUsing high-throughput sequencing, we provide the nearly complete mitochondrial genomes for 26 Loricariidae and one Callichthyidae species. Structural features were highly conserved. A notable exception was identified in the monophyletic clade comprising species of the Hemiancistrus, Hypostomini and Peckoltia-clades, a ~60 nucleotide-long deletion encompassing the seven nucleotides at the 3′ end of the Conserved Sequence Block (CSB) D of the control region. The expression of mitochondrial genes followed the usual punctuation pattern. Heteroplasmic sites were identified in most species. The retrieved phylogeny strongly corroborates the currently accepted tree, although bringing to debate the relationship between Schizolecis guntheri and Pareiorhaphis garbei, and highlighting the low genetic variability within the Peckoltia-clade, an eco-morphologically diverse and taxonomically problematic group.ConclusionsHerein we have launched the use of high-throughput mitochondrial genomics in the studies of the Loricarioidei species. The new genomic resources reduce the information gap on the molecular diversity of Neotropical fish fauna, impacting the capacity to investigate a variety of aspects of the molecular ecology and evolution of these fishes. Additionally, the species showing the partial CSB-D are candidate models to study the replication and transcription of vertebrate mitochondrial genome.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3709-3) contains supplementary material, which is available to authorized users.

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“… 87 However, ABC transporters excrete toxic metabolites 87 – 89 and protect against external pollutants. 90 , 91 In addition ABC10 protects against oxidative stress. 92 Radiation is known to cause oxidative stress, 93 and reactive oxygen species (ROS) production is thought to induce the BE (eg, in the study by Riganti 94 ).…”

Section: Discussionmentioning

confidence: 99%

Biological Entanglement–Like Effect After Communication of Fish Prior to X-Ray Exposure

et al. 2018

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The phenomenon by which irradiated organisms including cells in vitro communicate with unirradiated neighbors is well established in biology as the radiation-induced bystander effect (RIBE). Generally, the purpose of this communication is thought to be protective and adaptive, reflecting a highly conserved evolutionary mechanism enabling rapid adjustment to stressors in the environment. Stressors known to induce the effect were recently shown to include chemicals and even pathological agents. The mechanism is unknown but our group has evidence that physical signals such as biophotons acting on cellular photoreceptors may be implicated. This raises the question of whether quantum biological processes may occur as have been demonstrated in plant photosynthesis. To test this hypothesis, we decided to see whether any form of entanglement was operational in the system. Fish from 2 completely separate locations were allowed to meet for 2 hours either before or after which fish from 1 location only (group A fish) were irradiated. The results confirm RIBE signal production in both skin and gill of fish, meeting both before and after irradiation of group A fish. The proteomic analysis revealed that direct irradiation resulted in pro-tumorigenic proteomic responses in rainbow trout. However, communication from these irradiated fish, both before and after they had been exposed to a 0.5 Gy X-ray dose, resulted in largely beneficial proteomic responses in completely nonirradiated trout. The results suggest that some form of anticipation of a stressor may occur leading to a preconditioning effect or temporally displaced awareness after the fish become entangled.

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The liver transcriptome of suckermouth armoured catfish (Pterygoplichthys anisitsi, Loricariidae): Identification of expansions in defensome gene families

Cited by 14 publications

References 60 publications

A prototype of the mammalian sulfotransferase 1 (SULT1) family in <i>Xenopus laevis</i>: molecular and enzymatic properties of <i>Xl</i>SULT1B.S

A prototype of the mammalian sulfotransferase 1 (SULT1) family in <i>Xenopus laevis</i>: molecular and enzymatic properties of <i>Xl</i>SULT1B.S

Reducing the information gap on Loricarioidei (Siluriformes) mitochondrial genomics

Biological Entanglement–Like Effect After Communication of Fish Prior to X-Ray Exposure

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