Biochemical properties of the fibrinogen component of a fibrin glue before and after severe dry heat treatment

Abstract

Functional biochemical properties of 5 batches of the fibrinogen component of a fibrin glue produced by the ZLB Central Laboratory, Bern, each consisting of 4 different in-process samples (taken after the first and second precipitation step, lyophilization, and dry-heat treatment) were studied in vitro. We focused our attention on the effect of the anti-viral treatment of the lyophilized product by dry heat for 1 h at 100 degrees C. A slight reduction in maximal turbidity of all heat-treated samples was observed during the clotting assay compared to nontreated samples. Treatment with dry heat did not result in generation of fibrinogen fragments that might accelerate tissue-plasminogen-activator (t-PA)-enhanced plasminogen to plasmin conversion. The time course of fibrin cross-linking by factor XIII showed no differences between heated and unheated samples. This result indicates that exposure of the fibrinogen component to severe heat neither reduced activity of factor XIIIa nor affected the correct alignment of cross-linking sites in polymerized fibrin. Incubation of fibrinogen with thrombin, plasminogen, and t-PA resulted in a slightly enhanced degradation of fibrin derived from the heat-treated samples. The amount of residual moisture, determined to be within the range of 0.6-2.1% before heat treatment, did not influence clotting, cross-linking, and fibrinolysis parameters. In conclusion, the virus inactivation treatment by dry heat for 1 h at 100 degrees C induces no significant alterations of the in vitro biochemical properties of the fibrinogen component of this fibrin glue.

Tratamento de lesões condrais no joelho com condrócitos autólogos embebidos em arcabouço de fibrina. Avaliação clínica e funcional

“…6 Fibrin scaffolds are formed when two components derived from blood plasma come together (thrombin and fibrinogen) to build a specific matrix that offers a good environment for chondrocytes to proliferate. 8,16,17 Injuries to the medial femoral condyle generally obtained better results, after a 3-year follow up, than those in the lateral condyle, but not than those in the patella. 18 In our study, we had results similar to those in the literature.…”

“…6 Os arcabouços de fibrina são formados por dois componentes derivados do plasma sanguíneo (trombina e fibrinogênio) que, juntos, constroem uma matriz específica que oferece bom ambiente para a proliferação de condrócitos. 8,16,17 As lesões no côndilo femoral medial tenderam a apresentar resultados melhores após 3 anos de acompanhamento do que as do côndilo lateral, mas não as da patela. 18 Nosso estudo obteve resultados semelhantes aos da literatura.…”

Experimental Study on the Viscosity and Adhesive Performance of Exogenous Liquid Fibrin Glue

“…The two FG types have different preservation requirements when freeze-dried, and also for preservation period and temperature. Although only few reports describe the relationship between these requirements and fibrinogen activity, 10 – 12) as to what extent they derive from the methods by which FG is manufactured remains unclear. Since BP contains greater amount of thrombin, the viscosity of BP might be superior to that of BH, however, the viscosity test of solution A in this report was not related to thrombin.…”