Biochemical Parameters of Guinea Pig Perilymph Sampled According to Scala and Following Sound Presentation

Abstract

JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. The National Institute of Environmental Health Sciences (NIEHS) andBrogan & Partners are collaborating with JSTOR to digitize, preserve and extend access to Environmental Health Perspectives.Guinea pigs were exposed to sound varying from 2 to 8 kHz in frequency and 80-100 dB (SPL) in intensity for periods of 1 hr. The biochemical parameters, glucose, sodium, total protein, and the glycolytic enzymes, aldolase, phosphohexose isomerase, and total LDH as well as isozymes of the latter were ascertained for blood serum, perilymph, and, in some instances, cerebrospinal fluid. The three enzymes occurred at lower levels in perilymph as compared to blood serum. Except for a small difference in serum total protein, sound presentation incurred no significant effect on any of the above parameters. Definite differences in several metabolites were discerned for perilymph sampled according to scala and which were independent of the respective acoustical treatments. Thus, as compared to the scale tympani, the scala vestibule perilymph displayed a higher glucose content and a diminished total LDH level and of the latter isozymes, LDHM ranged lower and LDH2, higher. As further evidence pointing to cerebrospinal fluid as the possible origin of perilymph, similarities in glucose contents and LDH isozyme patterns were noted for both fluids.

Proteome Analysis of Human Perilymph Using an Intraoperative Sampling Method

“…Some groups studied the composition of perilymph or other parts of the inner ear like the sensory epithelium in humans and animals. 10,11 For human perilymph analysis, post-mortem samples were used in some studies. 12 Thalmann and Arrer were pioneers in the investigation of perilymph composition performed by gel electrophoresis, resulting in first preliminary perilymph protein profiles for humans.…”