Biochemical, molecular characterization and growth promoting effects of phosphate solubilizing Pseudomonas sp. isolated from weeds grown in salt range of Pakistan

Naz, Irum; Bano, Asghari

doi:10.1007/s11104-010-0372-8

Cited by 32 publications

(16 citation statements)

References 30 publications

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“…The phosphate solubilization phenotype is widespread among the members of Pseudomonas species including P. stutzeri (Peix et al, 2003;Naz and Bano, 2010;Sashidar and Podile, 2010) and Azospirillum species (Seshardi et al, 2000;Rodriguez et al, 2004;El-Komy, 2005;Venieraki et al, 2011). Our data revealed that all the P. stutzeri strains exhibited the capacity for solubilization of insoluble mineral phosphate while three (Gr60, Gr61, and Gr66) of eight Azospirillum isolates showed no such property.…”

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confidence: 56%

Characterization of nitrogen-fixing bacteria isolated from field-grown barley, oat, and wheat

et al. 2011

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Diazotrophic bacteria were isolated from the rhizosphere of field-grown Triticum aestivum, Hordeum vulgare, and Avena sativa grown in various regions of Greece. One isolate, with the highest nitrogen-fixation ability from each of the eleven rhizospheres, was selected for further characterisation. Diazotrophic strains were assessed for plant-growth-promoting traits such as indoleacetic acid production and phosphate solubilisation. The phylogenies of 16S rRNA gene of the selected isolates were compared with those based on dnaK and nifH genes. The constructed trees indicated that the isolates were members of the species Azospirillum brasilense, Azospirillum zeae, and Pseudomonas stutzeri. Furthermore, the ipdC gene was detected in all A. brasilence and one A. zeae isolates. The work presented here provides the first molecular genetic evidence for the presence of culturable nitrogen-fixing P. stutzeri and A. zeae associated with field-grown A. sativa and H. vulgare in Greece.

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confidence: 56%

Characterization of nitrogen-fixing bacteria isolated from field-grown barley, oat, and wheat

et al. 2011

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“…A BLAST comparison of the corresponding sequences against the GreenGenes database has shown that these OTUs are most closely related to Pseudomonas putida and Pseudomonas wynnii / graminis , respectively. P. putida strains have been reported to enhance plant growth by: 1) producing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, siderophores and phytohormones, 2) mobilising phosphorus, and 3) fixing nitrogen [34], [35], [36], [37]. Pseudomonas wynnii and Pseudomonas graminis are also known to fix nitrogen and Paenibacillus spp.…”

Section: Resultsmentioning

confidence: 99%

Activation of the Jasmonic Acid Plant Defence Pathway Alters the Composition of Rhizosphere Bacterial Communities

et al. 2013

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Jasmonic acid (JA) signalling plays a central role in plant defences against necrotrophic pathogens and herbivorous insects, which afflict both roots and shoots. This pathway is also activated following the interaction with beneficial microbes that may lead to induced systemic resistance. Activation of the JA signalling pathway via application of methyl jasmonate (MeJA) alters the composition of carbon containing compounds released by roots, which are implicated as key determinants of rhizosphere microbial community structure. In this study, we investigated the influence of the JA defence signalling pathway activation in Arabidopsis thaliana on the structure of associated rhizosphere bacterial communities using 16S rRNA gene amplicon pyrosequencing. Application of MeJA did not directly influence bulk soil microbial communities but significant changes in rhizosphere community composition were observed upon activation of the jasmonate signalling pathway. Our results suggest that JA signalling may mediate plant-bacteria interactions in the soil upon necrotrophic pathogen and herbivorous insect attacks.

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“…16S rRNA gene sequence analysis is an authentic technique used to study bacterial isolate at species level and allows their identification as well as prediction of phylogenetic relationship (Naz and Bano 2010). Sequence analysis of potential phosphate solubilizing Pseudomonas isolate An-15-Mg revealed that it showed 99% similarity with Pseudomonas aeruginosa (CP017353).…”

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confidence: 99%

Isolation of phosphate solubilizing Pseudomonas strains from apple rhizosphere in the Trans Himalayan region of Himachal Pradesh, India

Sharma

Pal

Kaur

2017

Preprint

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Total fifteen phosphate solubilizing bacteria were isolated from rhizosphere soil of apple tree on Kings' B medium belonging to genus Pseudomonas spp. They were characterized on the basis of morphological and biochemical characteristics. Preliminary selection as phosphate solubilizing bacteria were done on the basis of formation of transparent zone around the colony on Pikovskaya's agar medium containing 0.5% triclcium phosphate (TCP).Maximum in vitro phosphate solubilization on Pikovskaya's agar plates after 72h incubation at 28 0 C was shown by An-15-Mg (46 mm), whereas in case of broth, again this strain showed maximum tricalcium phosphate (TCP) solubilization (76 µg/ml). The pH of each inoculated broth was recorded daily and dropped significantly (pH 7.0-3.99). All the Pseudomonas isolates were further evaluated for overall plant growth promoting traits. Greatest siderophore activity was exhibited by An-14-Mg (71.23 %SU) followed by An-15-Mg (70.12 %SU) which were statiscally at par with each other. Whereas, maximum IAA production was observed again in An-15-Mg (95 µg/ml).This isolate also showed the maximum production of HCN and ammonia. 16S rDNA and phylogenetic analysis showed that strain An-15-Mg exhibits 99% level of similarity with Pseudomonas aeruginosa. Therefore, it was designated as Pseudomonas aeruginosa strain An-15-Mg. HPLC analysis showed that the Pseudomonas aeruginosa strain An-15-Mg produced maximum concentration of succinic acid, malonic, citric and malic acid with small amounts of schimic, quinic, tartaric, fumaric and lactic acids. The strain An-15-Mg possessed phosphate solubilization as major PGP trait along with different PGP traits. The potential phosphate solubilizing strain of Pseudomonas aeruginosa was reported first time as PGPR which lives in close association with apple tree without harming the plant, therefore could be used as a promising phosphate solubilizer and biofertilizer in apple crop grown in high hills of Himachal Pradesh.

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Biochemical, molecular characterization and growth promoting effects of phosphate solubilizing Pseudomonas sp. isolated from weeds grown in salt range of Pakistan

Cited by 32 publications

References 30 publications

Characterization of nitrogen-fixing bacteria isolated from field-grown barley, oat, and wheat

Characterization of nitrogen-fixing bacteria isolated from field-grown barley, oat, and wheat

Activation of the Jasmonic Acid Plant Defence Pathway Alters the Composition of Rhizosphere Bacterial Communities

Isolation of phosphate solubilizing Pseudomonas strains from apple rhizosphere in the Trans Himalayan region of Himachal Pradesh, India

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