Characterization of nitrogen-fixing bacteria isolated from field-grown barley, oat, and wheat

Venieraki, Anastasia; Dimou, Maria; Vezyri, Eleni; Kefalogianni, Io; Argyris, N.; Liara, Georgia; Pergalis, Panagiotis; Chatzipavlidis, Iordanis; Katinakis, Panagiotis

doi:10.1007/s12275-011-0457-y

Cited by 30 publications

(18 citation statements)

References 68 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The in vitro acetylene reduction capacity is indirectly considered to be at par with atmospheric N2-fixing ability of the isolates. Thus, acetylene reduction trait is only exhibited by isolate KR-29 and the amount of acetylene reduction (462.95 ηMol mg -1 protein h -1 ) was found similar to that already reported in diazotrophic bacteria isolated from field-grown barley wheat and oat (Venieraki et al, 2011). On the other hand, the inorganic tricalcium phosphate solubilized in vitro by isolates KP-21 and KR-29 (34.48 to 37.15 µg mL -1 , respectively) was in agreement with the outcomes of Oliveira et al (2009) who found the soluble phosphate range of 4-200 µg mL -1 in phosphate solubilizing isolates purified from maize rhizosphere.…”

Section: Discussionsupporting

confidence: 86%

First Report of Providencia vermicola Strains Characterized for Enhanced Rapeseed Growth Attributing Parameters

Hussain

Hameed

Shahid

et al. 2015

IJAB

View full text Add to dashboard Cite

Ever-increasing prices of synthetic fertilizers in developing countries put a huge burden on farmer community in terms of per unit cost of production. Supplementing chemical fertilizers with bacterial inoculants offers an alternative strategy to sustain yield by reducing cost of produce. Thus, in current study, two Providencia vermicola strains KP-21 and KR-29 were identified through 16S rRNA gene sequence analysis. The KP-21 and KR-29 strains were originally isolated from rhizosphere of rapeseed grown in Peshawar and Rawalakot, respectively. Morphologically, both strains were similar with round creamy colonies, short rod and Gram-negative cells under light microscope. P. vermicola KP-21 was found promising in phosphate soubilization and auxin biosynthesis. Likewise, strain P. vermicola KR-29 was found to be a potential phosphate solubilizer, auxin producer in addition to its considerable acetylene reduction capacity. High performance liquid chromatography (HPLC) analysis revealed that strain KP-21 produced gluconic and lactic acids, while strain KR-29 synthesized gluconic, malic and lactic acids in considerable amounts. Both strains densely colonized rapeseed rhizosphere area as depicted in transmission electron micrographs. Moreover, the strains significantly (P≤0.05) augmented rapeseed root and shoot length, dry weight, and phosphorus contents, while a significant increase in root and shoot nitrogen contents was only recorded in plants inoculated with strain KR-29 as compared to non-inoculated plants. We concluded that P. vermicola strains are being reported for the first time as rapeseed growth promoting agents. In addition, strain KP-21 and KR-29 were prospective PGPR rhizotypes which were recommended to be evaluated further under field conditions comprising of different agro-climatic locations before using them as commercial bio-inoculants.

show abstract

Section: Discussionsupporting

confidence: 86%

First Report of Providencia vermicola Strains Characterized for Enhanced Rapeseed Growth Attributing Parameters

Hussain

Hameed

Shahid

et al. 2015

IJAB

View full text Add to dashboard Cite

show abstract

“…The symbiotic Rhizobia-legume system is the major contributor of biologically fixed nitrogen as compared with non-symbiotic nitrogen-fixing bacteria [157]. The system is estimated to contribute 1.44 × 10 8 metric tons of nitrogen per year globally [158].…”

Section: Evaluation Of the Nitrogen Fixing Capability Of Isolated Strmentioning

confidence: 99%

Isolation and Characterization of Nitrogen Fixing Rhizobia from Cultivated and Uncultivated Soils of Northern Tanzania

Simon¹,

Mtei²,

Gessesse³

et al. 2014

AJPS

View full text Add to dashboard Cite

Soil bacteria 1 called rhizobia are gram-negative capable to colonize the soil immediately surrounding roots under the influence of the plant "rhizosphere" and reduce atmospheric nitrogen into the form available to plants through nitrogen fixation process. Nitrogen is the most limiting and supplied nutrient to most plants, and the determinant of plant growth. Legumes differ with most plants because they have access to nitrogen from both mineral and symbiotic sources. Small-scale farmers who are the major legume producers in Africa rarely apply fertilizers during legume production. Hence, the crop is largely dependent on fixed nitrogen from native nitrogen fixers. Isolation of rhizobia for legume production has been given a little attention in Africa due to inadequate research or negligence of researchers and unawareness of its potential in legume production as well as lack of an intention from skilled personnel to popularize the technology. Evaluation of effectiveness of isolated rhizobia is essential for inoculants preparation, host specificity recommendation and symbiotic effectiveness. The isolation, determination of their population in the soil and assessing factors affecting their population and testing the effectiveness of native nitrogen fixers with respect to right trap host crop are given a special attention in this review.

show abstract

“…Pair-wise comparison of the 16S rRNA revealed that most of the strains (Gr16, Gr17, Gr18, Gr19, Gr20, Gr21, Gr45, Gr46 and Gr50) shared homology to P. stutzeri strains exceeding 99.5% while both isolates Gr57 and Gr65 exhibited sequence similarities ranging from 97% to 97.2%, respectively when compared to type strains P. stutzeri and diazotrophic reference P. stutzeri A1501 and DSM4166 strains. Dissimilarity of the 16S rRNA genes more than 1.3% is a strong indication that the isolates under consideration may belong to two groups of different Pseudomonas species [11]. To confirm these indices, a 16S rRNA gene phylogenetic tree was constructed (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

“…Recently, this belief has been revised, since several nitrogen-fixing strains belonging to P. stutzeri have been isolated and characterized from the rhizosphere of gramineous plants like sorghum [7], [8], rice [9], wheat [10] and barley [11]. A diazotrophic Pseudomonas , P. azotifigens , has also been isolated from a compost pile [12].…”

Section: Introductionmentioning

confidence: 99%

The Nitrogen-Fixation Island Insertion Site Is Conserved in Diazotrophic Pseudomonas stutzeri and Pseudomonas sp. Isolated from Distal and Close Geographical Regions

et al. 2014

Self Cite

View full text Add to dashboard Cite

The presence of nitrogen fixers within the genus Pseudomonas has been established and so far most isolated strains are phylogenetically affiliated to Pseudomonas stutzeri. A gene ortholog neighborhood analysis of the nitrogen fixation island (NFI) in four diazotrophic P. stutzeri strains and Pseudomonas azotifigens revealed that all are flanked by genes coding for cobalamin synthase (cobS) and glutathione peroxidise (gshP). The putative NFIs lack all the features characterizing a mobilizable genomic island. Nevertheless, bioinformatic analysis P. stutzeri DSM 4166 NFI demonstrated the presence of short inverted and/or direct repeats within both flanking regions. The other P. stutzeri strains carry only one set of repeats. The genetic diversity of eleven diazotrophic Pseudomonas isolates was also investigated. Multilocus sequence typing grouped nine isolates along with P. stutzeri and two isolates are grouped in a separate clade. A Rep-PCR fingerprinting analysis grouped the eleven isolates into four distinct genotypes. We also provided evidence that the putative NFI in our diazotrophic Pseudomonas isolates is flanked by cobS and gshP genes. Furthermore, we demonstrated that the putative NFI of Pseudomonas sp. Gr65 is flanked by inverted repeats identical to those found in P. stutzeri DSM 4166 and while the other P. stutzeri isolates harbor the repeats located in the intergenic region between cobS and glutaredoxin genes as in the case of P. stutzeri A1501. Taken together these data suggest that all putative NFIs of diazotrophic Pseudomonas isolates are anchored in an intergenic region between cobS and gshP genes and their flanking regions are designated by distinct repeats patterns. Moreover, the presence of almost identical NFIs in diazotrophic Pseudomonas strains isolated from distal geographical locations around the world suggested that this horizontal gene transfer event may have taken place early in the evolution.

show abstract

Characterization of nitrogen-fixing bacteria isolated from field-grown barley, oat, and wheat

Cited by 30 publications

References 68 publications

First Report of Providencia vermicola Strains Characterized for Enhanced Rapeseed Growth Attributing Parameters

First Report of Providencia vermicola Strains Characterized for Enhanced Rapeseed Growth Attributing Parameters

Isolation and Characterization of Nitrogen Fixing Rhizobia from Cultivated and Uncultivated Soils of Northern Tanzania

The Nitrogen-Fixation Island Insertion Site Is Conserved in Diazotrophic Pseudomonas stutzeri and Pseudomonas sp. Isolated from Distal and Close Geographical Regions

Contact Info

Product

Resources

About