Biochemical, medical, and environmental applications of field-ionization and field-desorption mass spectrometry

Schulten, H.‐R.

doi:10.1016/0020-7381(79)80087-x

Cited by 242 publications

(56 citation statements)

References 236 publications

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“…, and NO 3 Ϫ adducts of oligosaccharides provide no structural information, i.e., they yield the respective anions as the main product ions. However, determination of linkage types is achieved by analysis of structurally-informative diagnostic peaks offered by negative ion PSD spectra of chloride adducts of oligosaccharides, whereas the relative peak intensities of pairs of diagnostic fragment ions allow differentiation of anomeric configurations of glycosidic bonds.…”

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confidence: 99%

MALDI linear-field reflectron TOF post-source decay analysis of underivatized oligosaccharides: Determination of glycosidic linkages and anomeric configurations using anion attachment

Guan

Cole

2008

J. Am. Soc. Mass Spectrom.

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Six different anionic species (fluoride, chloride, bromide, iodide, nitrate, and acetate) are tested for their abilities to form anionic adducts with neutral oligosaccharides that are detectable by MALDI-TOF mass spectrometry. Fluoride and acetate cannot form anionic adducts with the oligosaccharides in significant yields. However, bromide, iodide, and nitrate anionic adducts consistently appear in higher abundances relative to [M Ϫ H] Ϫ , just like the highly stable chloride adducts. Post-source decay (PSD) decompositions of Br Ϫ , IϪ , and NO 3 Ϫ adducts of oligosaccharides provide no structural information, i.e., they yield the respective anions as the main product ions. However, determination of linkage types is achieved by analysis of structurally-informative diagnostic peaks offered by negative ion PSD spectra of chloride adducts of oligosaccharides, whereas the relative peak intensities of pairs of diagnostic fragment ions allow differentiation of anomeric configurations of glycosidic bonds. Thus, simultaneous identification of the linkage types and anomeric configurations of glycosidic bonds is achieved. Our data indicate that negative ion PSD fragmentation patterns of chloride adducts of oligosaccharides are mainly determined by the linkage types. Correlation may exist between the linkage positions and fragmentation mechanisms and/or steric requirements for both cross-ring and glycosidic bond fragmentations. PSD of the chloride adducts of saccharides containing a terminal Glc␣1-2Fru linkage also yields chlorine-containing fragment ions which appear to be specifically diagnostic for a fructose linked at the 2-position on the reducing end. This also allows differentiation from saccharides with a 1-1 linked pyranose on the same position. ollowing the advances in proteomics, there is a growing interest in the importance of glycomics, i.e., the study of the breadth of sugar forms (structure and function of glycans) in biological organisms. Carbohydrates (or saccharides) are the most abundant biological compounds found on earth. They are well known as energy reservoirs and structural materials in cell walls. However, it has become clear that oligosaccharides and glycoconjugates (e.g., glycoproteins and glycolipids) serve as crucial mediators for a wide variety of complex cellular events [1]. Carbohydrates also play an important role in specific molecular recognition, protein folding, stability, and pharmacokinetics due to their great structural diversity. Moreover, glycosylation is a ubiquitous form of post-translational modification to both proteins and lipids.Gaining a clear understanding of the crucial biological roles of oligosaccharides requires complete structural characterization of carbohydrates or glycoconjugates, which includes determinations of the numbers and types of monosaccharide units, ring substituents, sequences, branching, linkage positions, and anomeric configurations between adjacent monosaccharide units. In most cases, merely knowing the monosaccharide sequence is inadequate; thus, unam...

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confidence: 99%

MALDI linear-field reflectron TOF post-source decay analysis of underivatized oligosaccharides: Determination of glycosidic linkages and anomeric configurations using anion attachment

Guan

Cole

2008

J. Am. Soc. Mass Spectrom.

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“…All spectra were produced using direct heating of the emitter by a heating current. The sample was transferred to the emitter by the modified syringe technique [13] and in general 1 to 3 jug of sample material were deposited on the center of the front side of the emitter. The applied potentials were -f 8 kV for the field anode and -4 kV for the opposing cathode plate.…”

Section: Methodsmentioning

confidence: 99%

“…lb). This cationization [13] of the molecule is the favoured process in the case of compound 2 (m/z 899), 3 (m\z 923) and 4 (m/z 905). It is noteworthy that in all experiments the impurities of alkali salts present in the authentic samples were sufficient for cationization.…”

Section: Introductionmentioning

confidence: 98%

“…In general, it appears that FD MS is a powerful method for the detection, identification and quantification of highly polar compounds in biochemical, medical and environmental analysis [13].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, for the spectrum in Fig. 1 a the section of the total desorption process at the best anode temperature [13] (Fig. lb).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Protected Deoxyribonucleotides by Field Desorption Mass Spectrometry in Fractions from High Performance Liquid Chromatography

Schiebel¹,

Schulten²

1981

Zeitschrift Für Naturforschung B

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Abstract Protected deoxyribonucleotides were identified by field desorption mass spectrometry in eluate fractions from high performance liquid chromatography. The production of abundant cationized molecules and the formation of structural significant fragments by thermally/field induced processes allowed a direct and unambiguous identification of the synthesized products. In addition, indications can be obtained on both organic and inorganic impurities.

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Multiple cationization of polyethylene glycols in field desorption mass spectrometry: a new approach to extend the mass scale on sector mass spectrometers

Guo

Fokkens

Peeters

et al. 1999

Rapid Commun. Mass Spectrom.

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Biochemical, medical, and environmental applications of field-ionization and field-desorption mass spectrometry

Cited by 242 publications

References 236 publications

MALDI linear-field reflectron TOF post-source decay analysis of underivatized oligosaccharides: Determination of glycosidic linkages and anomeric configurations using anion attachment

MALDI linear-field reflectron TOF post-source decay analysis of underivatized oligosaccharides: Determination of glycosidic linkages and anomeric configurations using anion attachment

Identification of Protected Deoxyribonucleotides by Field Desorption Mass Spectrometry in Fractions from High Performance Liquid Chromatography

Multiple cationization of polyethylene glycols in field desorption mass spectrometry: a new approach to extend the mass scale on sector mass spectrometers

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