Biochemical investigations after burning injury: Complement system, protease-antiprotease balance and acute-phase reactants

Faymonville, Marie; Micheels, Jean; Bodson, Lucien; Jacquemin, Dominique; Lamy, Maurice; Adam, Albert; Duchateau, Jean

doi:10.1016/0305-4179(87)90252-x

Cited by 19 publications

(5 citation statements)

References 18 publications

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“…Since our investigation addresses solely wound dressing primarily used in burn surgery, we will subsequently focus on the corresponding studies. Some of previous studies on burn patients showed that complement level was reduced, whereas others observed the activated complement system . Van de Goot et al .…”

Section: Discussionmentioning

confidence: 95%

Hemocompatibility of different burn wound dressings

Denzinger

Held

Scheffler

et al. 2019

Wound Repair Regeneration

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A variety of wound dressing are available for burns. Furthermore, although their impacts on wound healing have been studied sufficiently, their effects on blood remain unclear. Meanwhile, this aspect is extremely important, since blood interacts with the wound dressing, especially in extensive burn injuries. Therefore, the aim of this study is to evaluate the hemocompatibility and immunogenicity of different burn wound dressings. Accordingly, human whole blood (n = 5) was anticoagulated with heparin, treated with different wound dressings and incubated at 37 C for 30 minutes. Different parameters for coagulation and hemocompatibility were evaluated before and after incubation. Consequently, Jelonet, Xenoderm, and Matriderm showed higher TAT-III concentrations, Jelonet, Xenoderm, EZ Derm, and Matriderm were higher β-thromboglobulin; EZ Derm and Burntec showed higher SC5b-9 concentrations after incubation with whole blood. Our ex vivo study provided initial insights into the hemocompatibility and immunogenicity of different burn wound dressings. Moreover, Xenografts (Xenoderm and EZ Derm), Jelonet and Matriderm showed a hemostyptic effect, while EZ Derm and Burntec activated the complement system. Therefore, further studies must be conducted to analyze the possible effects in vivo. Hemocompatibility of different burn wound dressingsDenzinger et al.

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Section: Discussionmentioning

confidence: 95%

Hemocompatibility of different burn wound dressings

Denzinger

Held

Scheffler

et al. 2019

Wound Repair Regeneration

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“…The involvement of serine proteinases (elastase) in burn wounds and fluid was originally noted in 1987 and was later confirmed by the detection of proteinase inhibitors in burn fluid (20,21). SLPI is a potent inhibitor of serine proteases such as neutrophil elastase, cathepsin G, and mast cell chymase and is one of many innate immunity-associated proteins (22,23).…”

Section: Proteins Associated With the Inflammatory Responsementioning

confidence: 98%

Proteomic Investigation of Human Burn Wounds by 2D-Difference Gel Electrophoresis and Mass Spectrometry

Pollins¹,

Friedman²,

Nanney³

2007

Journal of Surgical Research

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Background-In humans, thermal cutaneous injury represents a serious traumatic event that induces a host of dynamic alterations. Unfortunately the molecular mechanisms that underlie these serious perturbations remain poorly understood. We applied a global analysis method to identify dynamically changing proteins within the burn environment, which could eventually become biomarkers or targets for treatment.

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“…Immediately after the burn trauma plasma levels of complement decrease, probably due to mechanical leakage, but 2 days after the injury a strong increase was detected. 1 Both complement and C-reactive protein (CRP) are upregulated systemically in response to burn injury [2][3][4][5] and are elevated for months after the trauma. 6 Systemic complement levels have been shown to be related to the severity of burn trauma and to the clinical outcome.…”

mentioning

confidence: 99%

Acute Inflammation is Persistent Locally in Burn Wounds: A Pivotal Role for Complement and C-Reactive Protein

Goot

Krijnen

Begieneman

et al. 2009

Journal of Burn Care & Research

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Severe burns can cause major complications, such as infection and deforming scar formation. Burn wounds induce an excessive inflammatory response. Serum levels of complement and the acute phase reactant C-reactive protein (CRP) are upregulated in response to burn injury and have been shown to be related to the severity of burn trauma and to the clinical outcome. However, complement and CRP have not been investigated on a tissue level locally at the site of the burn trauma. Protein levels and localization of complement activation product C3d and CRP were determined semi-quantitatively in burn eschar between 2 and 46 days after injury, using immunohistochemistry. CD68 and myeloperoxidase (MPO), markers for macrophages and neutrophilic granulocytes, respectively, were also analyzed on these biopsies. Skin biopsies of very recent surgical wounds (seconds old) served as controls. C3d and CRP are present at high levels in the burn wound. Protein levels of both mediators are significantly elevated up to at least 46 days after injury in comparison with control wounds. In line with this, neutrophils and macrophages infiltrate the burn wound in high numbers up to at least 46 days after injury. The excessive presence of the inflammatory mediators, complement and CRP, and the increased infiltration of neutrophils and macrophages in burn woundsup to 46 days after injury implicate a persistent ongoing acute inflammation locally in the burn wound up to weeks after the initial trauma. (J Burn Care Res 2009;30:274 -280)

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Biochemical investigations after burning injury: Complement system, protease-antiprotease balance and acute-phase reactants

Cited by 19 publications

References 18 publications

Hemocompatibility of different burn wound dressings

Hemocompatibility of different burn wound dressings

Proteomic Investigation of Human Burn Wounds by 2D-Difference Gel Electrophoresis and Mass Spectrometry

Acute Inflammation is Persistent Locally in Burn Wounds: A Pivotal Role for Complement and C-Reactive Protein

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