Biochemical Identification of
 Citrobacter
 Species Defined by DNA Hybridization and Description of
 Citrobacter gillenii
 sp. nov. (Formerly
 Citrobacter
 Genomospecies 10) and
 Citrobacter murliniae
 sp. nov. (Formerly
 Citrobacter
 Genomospecies 11)

Brenner, Don J.; O'Hara, C M; Grimont, Patrick A. D.; Janda, J. Michael; Falsen, Enevold; Aldová, E; Ageron, Elisabeth; Schindler, Jiří; Abbott, Sharon L.; Steigerwalt, A G

doi:10.1128/jcm.37.8.2619-2624.1999

Cited by 82 publications

(40 citation statements)

References 13 publications

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“…Based on DNA relatedness and biochemical studies, the genus Citrobacter has been divided into 11 genomospecies [15]. In C. freundii the resistance to L-lactams is mediated by production of a chromosomally encoded class C L-lactamase [8].…”

Section: Introductionmentioning

confidence: 99%

Cloning and sequencing of the β-lactamase gene and surrounding DNA sequences of Citrobacter braakii, Citrobacter murliniae, Citrobacter werkmanii, Escherichia fergusonii and Enterobacter cancerogenus

Naas¹

2002

FEMS Microbiology Letters

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To further identify the origins of plasmid-mediated cephalosporinases that are currently spreading worldwide, the chromosomal L-lactamase genes of Citrobacter braakii, Citrobacter murliniae, Citrobacter werkmanii reference strains and of Escherichia fergusonii and Enterobacter cancerogenus clinical isolates were cloned and expressed into Escherichia coli and sequenced. These L-lactamases had all a single pI value s 8 and conferred a typical AmpC-type resistance pattern in E. coli recombinant strains. The cloned inserts obtained from genomic DNAs of each strain encoded Ambler class C L-lactamases. The AmpC-type enzymes of C. murliniae, C. braakii and C. werkmanii shared 99%, 96% and 95% amino acid sequence identity, respectively, with chromosomal AmpC L-lactamases from Citrobacter freundii. The AmpC-type enzyme of E. cancerogenus shared 85% amino acid sequence identity with the chromosomal AmpC L-lactamase of Enterobacter cloacae OUDhyp and the AmpC-type enzyme of E. fergusonii shared 96% amino acid sequence identity with that of E. coli K12. The ampC genes, except for E. fergusonii, were associated with genes homologous to regulatory ampR genes of other chromosomal class C L-lactamases that explain inducibility of L-lactamase expression in these strains. This work provides further evidence of the molecular heterogeneity of class C L-lactamases.

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Section: Introductionmentioning

confidence: 99%

Cloning and sequencing of the β-lactamase gene and surrounding DNA sequences of Citrobacter braakii, Citrobacter murliniae, Citrobacter werkmanii, Escherichia fergusonii and Enterobacter cancerogenus

Naas¹

2002

FEMS Microbiology Letters

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“…Perchlorate was completely dismutated to chloride and oxygen. The strain JB101 closely resembled with other C. amalonaticus strains in utilization of sucrose, SIMMON's citrate, dulcitol, α-methyl-glucoside, and production of ornithine decarboxylase (ODC) and indole (O'HARA et al 1995, BRENNER et al 1999). However, a few important differences were noticed when compared to the other reported strains of C. amalonaticus.…”

Section: Metabolic Characterizationmentioning

confidence: 71%

“…Carbon source utilization-based testing protocols are reliable for phenotypic and biochemical characterization of Citrobacter spp. (BRENNER et al 1999). After testing positively for Gram-negative reaction on BIOLOG medium, both the Citrobacter strains were examined for their metabolic abilities.…”

Section: Metabolic Characterizationmentioning

confidence: 99%

Role of Citrobacter amalonaticus and Citrobacter farmeri in dissimilatory perchlorate reduction

Bardiya

Bae

2004

J. Basic Microbiol.

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The article deals with the novel physiological function of dissimilatory perchlorate reduction by strains JB101 and JB109 isolated from a laboratory-enriched mixed consortium originating from a sewage treatment facility. The biochemical and physiological data of the strains showed good correspondence with members of the family Enterobacteriaceae. The partial 16S rDNA sequence of the strains JB101 and JB109 had similarity of 99.8% to Citrobacter amalonaticus and 98% to Citrobacter farmeri, respectively. The results inferred the possibility of Citrobacter spp. to form an important group of dissimilatory perchlorate reducers among the gamma subclass of Proteobacteria, since the majority of the perchlorate reducers belong to two monophyletic groups, Dechloromonas and Dechlorosoma in beta subclass. The perchlorate-grown Citrobacter strains preferred perchlorate to nitrate as an electron acceptor unlike most of the reported dissimilatory perchlorate reducers.

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“…Paludibacter propionicigenes [24], Thermanaerovibrio acidaminovorans [25], Enterobacter cancerogenus [26], Citrobacter braakii [27] and Propionispora hippie [28]. Magnetospirillum sp.…”

Section: Molecular Determination Of Microbial Communitymentioning

confidence: 99%

Inocula selection in microbial fuel cells based on anodic biofilm abundance of Geobacter sulfurreducens

Sun

Rodrigues

Thygesen

et al. 2016

Chinese Journal of Chemical Engineering

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Microbial fuel cells (MFCs) rely on microbial conversion of organic substrates to electricity. The optimal performance depends on the establishment of a microbial community rich in electrogenic bacteria. Usually this microbial community is established from inoculation of the MFC anode chamber with naturally occurring mixed inocula. In this study, the electrochemical performance of MFCs and microbial community evolution were evaluated for three inocula including domestic wastewater (DW), lake sediment (LS) and biogas sludge (BS) with varying substrate loading (Lsub) and external resistance (Rext) on the MFC. The electrogenic bacterium Geobacter sulfurreducens was identified in all inocula and its abundance during MFC operation was positively linked to the MFC performance. The LS inoculated MFCs showed highest abundance (18 ± 1%) of G. sulfurreducens, maximum current density (Imax= 690 ± 30 mA•m-2) and coulombic efficiency (CE = 29 ± 1%) with acetate as the substrate. Imax and CE increased to 1780 ± 30 mA•m-2 and 58 ± 1%, respectively, after decreasing the Rext from 1000 Ω to 200 Ω, which also correlated to a higher abundance of G. sulfurreducens (21 ± 0.7%) on the MFC anodic biofilm. The data obtained contribute to understanding the microbial community response to Lsub and Rext for optimizing electricity generation in MFCs.

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Biochemical Identification of Citrobacter Species Defined by DNA Hybridization and Description of Citrobacter gillenii sp. nov. (Formerly Citrobacter Genomospecies 10) and Citrobacter murliniae sp. nov. (Formerly Citrobacter Genomospecies 11)

Cited by 82 publications

References 13 publications

Cloning and sequencing of the β-lactamase gene and surrounding DNA sequences of Citrobacter braakii, Citrobacter murliniae, Citrobacter werkmanii, Escherichia fergusonii and Enterobacter cancerogenus

Cloning and sequencing of the β-lactamase gene and surrounding DNA sequences of Citrobacter braakii, Citrobacter murliniae, Citrobacter werkmanii, Escherichia fergusonii and Enterobacter cancerogenus

Role of Citrobacter amalonaticus and Citrobacter farmeri in dissimilatory perchlorate reduction

Inocula selection in microbial fuel cells based on anodic biofilm abundance of Geobacter sulfurreducens

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