Biochemical, electrophysiological and immunological characterization of the venom from Centruroides baergi, a new scorpion species of medical importance in Mexico

Gómez-Ramírez, Ilse V.; Riaño-Umbarila, Lidia; Olamendi-Portugal, Timoteo; Restano-Cassulini, Rita; Possani, Lourival D.; Becerril, Baltazar

doi:10.1016/j.toxicon.2020.05.021

Cited by 9 publications

(2 citation statements)

References 27 publications

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“…They were limited to Isometrus maculatus [ 88 ] and Liocheles australasiae [ 89 ] venom. For other scorpion species, the venom has not been studied, but for some of them, the venom of scorpions of the same genus have be studied ( Centruroides [ 90 , 91 , 92 , 93 , 94 , 95 , 96 ], Opisthacanthus [ 97 , 98 ], Didymocentrus [ 99 ], Hormurus [ 100 , 101 ]). In France, these species or genera do not present serious complications during human envenomation, so their venom will not be further developed in the present review.…”

Section: Scorpion Venommentioning

confidence: 99%

French Scorpionism (Mainland and Oversea Territories): Narrative Review of Scorpion Species, Scorpion Venom, and Envenoming Management

Vaucel

Larréché

Paradis

et al. 2022

Toxins

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Sixty-seven scorpion species have been described in France and its territories, where they have been found to be heterogeneously distributed. Indeed, only one species can be found on Réunion Island, while 38 species exist in French Guiana. The number of stings is also heterogenous, with up to 90 stings per 100,000 inhabitants occurring annually. Scorpion species can frequently be determined through simple visual factors, including species of medical importance (i.e., Buthus, Centruroides and Tityus). Scorpion venom is composed of local enzymes and peptides with a cysteine-stabilized α/β motif (NaTxs, Ktxs, Calcines), which allow for venom diffusion and the prey’s incapacitation, respectively. Harmful scorpion species are limited to Centruroides pococki in the French West Indies, which can induce severe envenoming, and the Tityus obscurus and Tityus silvestris in French Guiana, which can cause fatalities in children and can induce severe envenoming, respectively. Envenomation by one of these scorpions requires hospital monitoring as long as systemic symptoms persist. Typical management includes the use of a lidocaine patch, pain killers, and local antiseptic. In the case of heart failure, the use of dobutamine can improve survival, and pregnant women must consult an obstetrician because of the elevated risk of preterm birth or stillbirth. France does not have scorpion antivenom, as scorpion stings are generally not fatal.

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Section: Scorpion Venommentioning

confidence: 99%

French Scorpionism (Mainland and Oversea Territories): Narrative Review of Scorpion Species, Scorpion Venom, and Envenoming Management

Vaucel

Larréché

Paradis

et al. 2022

Toxins

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“…A recent characterization of this venom showed the presence of two main toxic components named CsEM1a and CsEd with abundances of 8% and 1.6%, respectively [ 5 ]. Like other toxins from Mexican scorpions, these two also modify the activity of mammalian sodium channels [ 6 , 7 , 8 ]. Although there is an antivenom of equine origin [ 9 ], the alternative of producing an antivenom based on antibody fragments of human origin is novel, with the advantage of eliminating arduous collections and sacrifice of thousands of scorpions as well as the use of horses.…”

Section: Introductionmentioning

confidence: 99%

Full Neutralization of Centruroides sculpturatus Scorpion Venom by Combining Two Human Antibody Fragments

Riaño-Umbarila

Romero-Moreno

Ledezma-Candanoza

et al. 2021

Toxins

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A fundamental issue of the characterization of single-chain variable fragments (scFvs), capable of neutralizing scorpion toxins, is their cross-neutralizing ability. This aspect is very important in Mexico because all scorpions dangerous to humans belong to the Centruroides genus, where toxin sequences show high identity. Among toxin-neutralizing antibodies that were generated in a previous study, scFv 10FG2 showed a broad cross-reactivity against several Centruroides toxins, while the one of scFv LR is more limited. Both neutralizing scFvs recognize independent epitopes of the toxins. In the present work, the neutralization capacity of these two scFvs against two medically important toxins of the venom of Centruroides sculpturatus Ewing was evaluated. The results showed that these toxins are recognized by both scFvs with affinities between 1.8 × 10−9 and 6.1 × 10−11 M. For this reason, their ability to neutralize the venom was evaluated in mice, where scFv 10FG2 showed a better protective capacity. A combination of both scFvs at a molar ratio of 1:5:5 (toxins: scFv 10FG2: scFv LR) neutralized the venom without the appearance of any signs of intoxication. These results indicate a complementary activity of these two scFvs during venom neutralization.

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The venom of the scorpion Centruroides limpidus, which causes the highest number of stings in Mexico, is neutralized by two recombinant antibody fragments

Fernández-Taboada¹,

Riaño-Umbarila

Corzo³

et al. 2021

Molecular Immunology

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Biochemical, electrophysiological and immunological characterization of the venom from Centruroides baergi, a new scorpion species of medical importance in Mexico

Cited by 9 publications

References 27 publications

French Scorpionism (Mainland and Oversea Territories): Narrative Review of Scorpion Species, Scorpion Venom, and Envenoming Management

French Scorpionism (Mainland and Oversea Territories): Narrative Review of Scorpion Species, Scorpion Venom, and Envenoming Management

Full Neutralization of Centruroides sculpturatus Scorpion Venom by Combining Two Human Antibody Fragments

The venom of the scorpion Centruroides limpidus, which causes the highest number of stings in Mexico, is neutralized by two recombinant antibody fragments

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